pLV3-U6-DTL-shRNA3-CopGFP-Puro plasmid

PVT101926

pLV3-U6-DTL-shRNA3-CopGFP-Puro plasmid Information

Plasmid name: pLV3-U6-DTL(human)-shRNA3-CopGFP-Puro
Alias: GCCTAGTAACAGTAACGAGTA
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-DTL-shRNA3-CopGFP-Puro plasmid Description

pLV3-U6-DTL-shRNA3-CopGFP-Puro This is a plasmid suitable for interference silencing in mammalian cells, and it can also be used for lentivirus packaging.

pLV3-U6-DTL-shRNA3-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct 241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 421 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt 481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg 541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta 661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta 721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga 781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg 841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt 901 aagaccaccg cacagcaagc ggccactgat cttcagacct ggaggaggag atatgaggga 961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc 1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc 1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct 1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag 1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca 1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg 1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa 1381 atctctggaa cagattggaa tcacacgacc tggatggagt gggacagaga aattaacaat 1441 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa 1501 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat 1561 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata 1621 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt 1681 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt 1741 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtATCGGTta 1801 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata 1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta 1921 tcgatactag tGAGGGCCTA TTTCCCATGA TTCCTTCATA TTTGCATATA CGATACAAGG 1981 CTGTTAGAGA GATAATTAGA ATTAATTTGA CTGTAAACAC AAAGATATTA GTACAAAATA 2041 CGTGACGTAG AAAGTAATAA TTTCTTGGGT AGTTTGCAGT TTTAAAATTA TGTTTTAAAA 2101 TGGACTATCA TATGCTTACC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT 2161 TGTGGAAAGG ACTCATCgct agcgaattcg GCCTAGTAAC AGTAACGAGT Attcaagaga 2221 TACTCGTTAC TGTTACTAGG Cttttttgga tccgcggccg cGaaggatct gcgatcgctc 2281 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg 2341 ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt 2401 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt 2461 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg 2521 ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt 2581 gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt 2641 aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag 2701 actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc 2761 gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac 2821 catggagagc gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac 2881 cctgaacggc gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg 2941 catgaccaac aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag 3001 ccacgtgatg ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc 3061 cttcctgcac gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga 3121 cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga 3181 cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat 3241 ccgcagcaac gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag 3301 cttcgcccgc accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca 3361 catgcacttc aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc 3421 cttccgccgc gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca 3481 cgccttcaag acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt 3541 ggacggcacc gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac 3601 atgcggtgac gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct 3661 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta 3721 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca 3781 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg 3841 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga 3901 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga 3961 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt 4021 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc 4081 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt 4141 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac 4201 ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga 4261 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt 4321 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa 4381 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt 4441 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct 4501 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg 4561 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc 4621 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg 4681 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct 4741 gctgccggct ctgcggcctc ttccgcgtct ccgccttcgc cctcagacga gtcggatctc 4801 cctttggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga 4861 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaa 4921 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 4981 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 5041 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 5101 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 5161 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa 5221 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 5281 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc 5341 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 5401 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 5461 ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg 5521 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 5581 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 5641 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 5701 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 5761 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 5821 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 5881 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 5941 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 6001 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 6061 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 6121 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 6181 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 6241 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 6301 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 6361 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 6421 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 6481 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 6541 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 6601 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 6661 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 6721 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 6781 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 6841 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 6901 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 6961 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 7021 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 7081 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 7141 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 7201 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 7261 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 7321 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 7381 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 7441 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 7501 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 7561 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 7621 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt 7681 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt 7741 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt 7801 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg 7861 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg 7921 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca 7981 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag 8041 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag 8101 tcacgacgtt gtaaaacgac ggccagtgcc aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???