pLV3-U6-EEF2-shRNA2-CopGFP-Puro plasmid

PVT104241

pLV3-U6-EEF2-shRNA2-CopGFP-Puro plasmid Information

Plasmid name: pLV3-U6-EEF2(human)-shRNA2-CopGFP-Puro
Alias: GCAGTACCTCAACGAGATCAA
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F(Hairpin structure)
Reverse primer:
Copy number: High
Induction:

pLV3-U6-EEF2-shRNA2-CopGFP-Puro plasmid Description

pLV3-U6-EEF2-shRNA2-CopGFP-Puro This is a plasmid suitable for interference silencing in mammalian cells, and it can also be used for lentivirus packaging.

pLV3-U6-EEF2-shRNA2-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct 241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 421 ccgaacaggg acttgaaagc gaaagggaaa ccagagGAGc tctctcgacg caggactcgg 481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt 541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg 601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa 661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg 721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca 781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa 841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa 901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag 961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt 1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg 1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac 1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct 1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct 1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg 1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa 1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa 1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa 1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac 1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag 1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc 1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga 1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtATCG 1801 GTtaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga 1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat 1921 tttatcgata ctagtGAGGG CCTATTTCCC ATGATTCCTT CATATTTGCA TATACGATAC 1981 AAGGCTGTTA GAGAGATAAT TAGAATTAAT TTGACTGTAA ACACAAAGAT ATTAGTACAA 2041 AATACGTGAC GTAGAAAGTA ATAATTTCTT GGGTAGTTTG CAGTTTTAAA ATTATGTTTT 2101 AAAATGGACT ATCATATGCT TACCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT 2161 ATCTTGTGGA AAGGACTCAT Cgctagcgaa ttcgGCAGTA CCTCAACGAG ATCAATTCAA 2221 GAGATTGATC TCGTTGAGGT ACTGCTTTTT Tggatccgcg gccgcGaagg atctgcgatc 2281 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg 2341 gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg 2401 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag 2461 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc 2521 gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc 2581 ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct 2641 aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac 2701 ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg 2761 tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct acgctagacg 2821 ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg 2881 gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc cccaagcagg 2941 gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc ccctacctgc 3001 tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc ggctacgaga 3061 accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc gagaagtacg 3121 aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg 3181 gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc accgacaaga 3241 tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac gtgctggtgg 3301 gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc gtggtggaca 3361 gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg ggccccatgt 3421 tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc gtggagtacc 3481 agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg tccaattctg 3541 ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga ggaagtcttc 3601 taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag cccacggtgc 3661 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg 3721 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc 3781 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg 3841 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg 3901 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga 3961 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg 4021 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg 4081 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc 4141 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc 4201 gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat tacaaaattt 4261 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg 4321 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt 4381 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg 4441 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc 4501 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg 4561 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt 4621 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc 4681 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg 4741 gcctgctgcc ggctctgcgg cctcttccgc gtctccgcct tcgccctcag acgagtcgga 4801 tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct 4861 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa 4921 cgaagataag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc 4981 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 5041 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 5101 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag 5161 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct 5221 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 5281 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag 5341 ctatcccgcc cctaactccg CCCATCCCGC CCCTAACTCC Gcccagttcc gcccattctc 5401 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 5461 agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc agagaccaaa 5521 ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 5581 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 5641 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 5701 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 5761 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 5821 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 5881 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 5941 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 6001 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 6061 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 6121 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 6181 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 6241 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 6301 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 6361 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 6421 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 6481 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 6541 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 6601 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 6661 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 6721 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 6781 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 6841 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 6901 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 6961 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 7021 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 7081 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 7141 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 7201 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 7261 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 7321 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 7381 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 7441 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 7501 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 7561 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 7621 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 7681 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 7741 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc 7801 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc 7861 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt 7921 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac 7981 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg 8041 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg 8101 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???