pLV3-U6-EFR3B-sgRNA2-Cas9-Puro plasmid

PVT89869

pLV3-U6-EFR3B-sgRNA2-Cas9-Puro plasmid Information

Plasmid name: pLV3-U6-EFR3B(human)-sgRNA2-Cas9-Puro
Alias: GAAGTTTGCCAACATCGAGG
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Gene editing
Fragment type: CRISPR
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-EFR3B-sgRNA2-Cas9-Puro plasmid Description

pLV3-U6-EFR3B-sgRNA2-Cas9-Puro This is a plasmid suitable for gene editing in mammalian cells, and it can also be used for lentivirus packaging.

pLV3-U6-EFR3B-sgRNA2-Cas9-Puro plasmid Sequence

1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta aggtaccgag ggcctatttc ccatgattcc ttcatatttg 2641 catatacgat acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag 2701 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta 2761 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc 2821 ttggctttat atatcttGTG GAAAGGACGA AACACCgGAA GTTTGCCAAC ATCGAGGgtt 2881 ttagagctaG AAAtagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc 2941 accgagtcgg tgcTTTTTTg aattcgctag ctaggtcttg aaaggagtgg gaattggctc 3001 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg 3061 ggtcggcaat tgatccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt 3121 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt 3181 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggacc ggttctagag 3241 cgctgccacc ATGGACAAGA AGTACAGCAT CGGCCTGGAC ATCGGCACCA ACTCTGTGGG 3301 CTGGGCCGTG ATCACCGACG AGTACAAGGT GCCCAGCAAG AAATTCAAGG TGCTGGGCAA 3361 CACCGACCGG CACAGCATCA AGAAGAACCT GATCGGAGCC CTGCTGTTCG ACAGCGGCGA 3421 AACAGCCGAG GCCACCCGGC TGAAGAGAAC CGCCAGAAGA AGATACACCA GACGGAAGAA 3481 CCGGATCTGC TATCTGCAAG AGATCTTCAG CAACGAGATG GCCAAGGTGG ACGACAGCTT 3541 CTTCCACAGA CTGGAAGAGT CCTTCCTGGT GGAAGAGGAT AAGAAGCACG AGCGGCACCC 3601 CATCTTCGGC AACATCGTGG ACGAGGTGGC CTACCACGAG AAGTACCCCA CCATCTACCA 3661 CCTGAGAAAG AAACTGGTGG ACAGCACCGA CAAGGCCGAC CTGCGGCTGA TCTATCTGGC 3721 CCTGGCCCAC ATGATCAAGT TCCGGGGCCA CTTCCTGATC GAGGGCGACC TGAACCCCGA 3781 CAACAGCGAC GTGGACAAGC TGTTCATCCA GCTGGTGCAG ACCTACAACC AGCTGTTCGA 3841 GGAAAACCCC ATCAACGCCA GCGGCGTGGA CGCCAAGGCC ATCCTGTCTG CCAGACTGAG 3901 CAAGAGCAGA CGGCTGGAAA ATCTGATCGC CCAGCTGCCC GGCGAGAAGA AGAATGGCCT 3961 GTTCGGAAAC CTGATTGCCC TGAGCCTGGG CCTGACCCCC AACTTCAAGA GCAACTTCGA 4021 CCTGGCCGAG GATGCCAAAC TGCAGCTGAG CAAGGACACC TACGACGACG ACCTGGACAA 4081 CCTGCTGGCC CAGATCGGCG ACCAGTACGC CGACCTGTTT CTGGCCGCCA AGAACCTGTC 4141 CGACGCCATC CTGCTGAGCG ACATCCTGAG AGTGAACACC GAGATCACCA AGGCCCCCCT 4201 GAGCGCCTCT ATGATCAAGA GATACGACGA GCACCACCAG GACCTGACCC TGCTGAAAGC 4261 TCTCGTGCGG CAGCAGCTGC CTGAGAAGTA CAAAGAGATT TTCTTCGACC AGAGCAAGAA 4321 CGGCTACGCC GGCTACATTG ACGGCGGAGC CAGCCAGGAA GAGTTCTACA AGTTCATCAA 4381 GCCCATCCTG GAAAAGATGG ACGGCACCGA GGAACTGCTC GTGAAGCTGA ACAGAGAGGA 4441 CCTGCTGCGG AAGCAGCGGA CCTTCGACAA CGGCAGCATC CCCCACCAGA TCCACCTGGG 4501 AGAGCTGCAC GCCATTCTGC GGCGGCAGGA AGATTTTTAC CCATTCCTGA AGGACAACCG 4561 GGAAAAGATC GAGAAGATCC TGACCTTCCG CATCCCCTAC TACGTGGGCC CTCTGGCCAG 4621 GGGAAACAGC AGATTCGCCT GGATGACCAG AAAGAGCGAG GAAACCATCA CCCCCTGGAA 4681 CTTCGAGGAA GTGGTGGACA AGGGCGCTTC CGCCCAGAGC TTCATCGAGC GGATGACCAA 4741 CTTCGATAAG AACCTGCCCA ACGAGAAGGT GCTGCCCAAG CACAGCCTGC TGTACGAGTA 4801 CTTCACCGTG TATAACGAGC TGACCAAAGT GAAATACGTG ACCGAGGGAA TGAGAAAGCC 4861 CGCCTTCCTG AGCGGCGAGC AGAAAAAGGC CATCGTGGAC CTGCTGTTCA AGACCAACCG 4921 GAAAGTGACC GTGAAGCAGC TGAAAGAGGA CTACTTCAAG AAAATCGAGT GCTTCGACTC 4981 CGTGGAAATC TCCGGCGTGG AAGATCGGTT CAACGCCTCC CTGGGCACAT ACCACGATCT 5041 GCTGAAAATT ATCAAGGACA AGGACTTCCT GGACAATGAG GAAAACGAGG ACATTCTGGA 5101 AGATATCGTG CTGACCCTGA CACTGTTTGA GGACAGAGAG ATGATCGAGG AACGGCTGAA 5161 AACCTATGCC CACCTGTTCG ACGACAAAGT GATGAAGCAG CTGAAGCGGC GGAGATACAC 5221 CGGCTGGGGC AGGCTGAGCC GGAAGCTGAT CAACGGCATC CGGGACAAGC AGTCCGGCAA 5281 GACAATCCTG GATTTCCTGA AGTCCGACGG CTTCGCCAAC AGAAACTTCA TGCAGCTGAT 5341 CCACGACGAC AGCCTGACCT TTAAAGAGGA CATCCAGAAA GCCCAGGTGT CCGGCCAGGG 5401 CGATAGCCTG CACGAGCACA TTGCCAATCT GGCCGGCAGC CCCGCCATTA AGAAGGGCAT 5461 CCTGCAGACA GTGAAGGTGG TGGACGAGCT CGTGAAAGTG ATGGGCCGGC ACAAGCCCGA 5521 GAACATCGTG ATCGAAATGG CCAGAGAGAA CCAGACCACC CAGAAGGGAC AGAAGAACAG 5581 CCGCGAGAGA ATGAAGCGGA TCGAAGAGGG CATCAAAGAG CTGGGCAGCC AGATCCTGAA 5641 AGAACACCCC GTGGAAAACA CCCAGCTGCA GAACGAGAAG CTGTACCTGT ACTACCTGCA 5701 GAATGGGCGG GATATGTACG TGGACCAGGA ACTGGACATC AACCGGCTGT CCGACTACGA 5761 TGTGGACCAT ATCGTGCCTC AGAGCTTTCT GAAGGACGAC TCCATCGACA ACAAGGTGCT 5821 GACCAGAAGC GACAAGAACC GGGGCAAGAG CGACAACGTG CCCTCCGAAG AGGTCGTGAA 5881 GAAGATGAAG AACTACTGGC GGCAGCTGCT GAACGCCAAG CTGATTACCC AGAGAAAGTT 5941 CGACAATCTG ACCAAGGCCG AGAGAGGCGG CCTGAGCGAA CTGGATAAGG CCGGCTTCAT 6001 CAAGAGACAG CTGGTGGAAA CCCGGCAGAT CACAAAGCAC GTGGCACAGA TCCTGGACTC 6061 CCGGATGAAC ACTAAGTACG ACGAGAATGA CAAGCTGATC CGGGAAGTGA AAGTGATCAC 6121 CCTGAAGTCC AAGCTGGTGT CCGATTTCCG GAAGGATTTC CAGTTTTACA AAGTGCGCGA 6181 GATCAACAAC TACCACCACG CCCACGACGC CTACCTGAAC GCCGTCGTGG GAACCGCCCT 6241 GATCAAAAAG TACCCTAAGC TGGAAAGCGA GTTCGTGTAC GGCGACTACA AGGTGTACGA 6301 CGTGCGGAAG ATGATCGCCA AGAGCGAGCA GGAAATCGGC AAGGCTACCG CCAAGTACTT 6361 CTTCTACAGC AACATCATGA ACTTTTTCAA GACCGAGATT ACCCTGGCCA ACGGCGAGAT 6421 CCGGAAGCGG CCTCTGATCG AGACAAACGG CGAAACCGGG GAGATCGTGT GGGATAAGGG 6481 CCGGGATTTT GCCACCGTGC GGAAAGTGCT GAGCATGCCC CAAGTGAATA TCGTGAAAAA 6541 GACCGAGGTG CAGACAGGCG GCTTCAGCAA AGAGTCTATC CTGCCCAAGA GGAACAGCGA 6601 TAAGCTGATC GCCAGAAAGA AGGACTGGGA CCCTAAGAAG TACGGCGGCT TCGACAGCCC 6661 CACCGTGGCC TATTCTGTGC TGGTGGTGGC CAAAGTGGAA AAGGGCAAGT CCAAGAAACT 6721 GAAGAGTGTG AAAGAGCTGC TGGGGATCAC CATCATGGAA AGAAGCAGCT TCGAGAAGAA 6781 TCCCATCGAC TTTCTGGAAG CCAAGGGCTA CAAAGAAGTG AAAAAGGACC TGATCATCAA 6841 GCTGCCTAAG TACTCCCTGT TCGAGCTGGA AAACGGCCGG AAGAGAATGC TGGCCTCTGC 6901 CGGCGAACTG CAGAAGGGAA ACGAACTGGC CCTGCCCTCC AAATATGTGA ACTTCCTGTA 6961 CCTGGCCAGC CACTATGAGA AGCTGAAGGG CTCCCCCGAG GATAATGAGC AGAAACAGCT 7021 GTTTGTGGAA CAGCACAAGC ACTACCTGGA CGAGATCATC GAGCAGATCA GCGAGTTCTC 7081 CAAGAGAGTG ATCCTGGCCG ACGCTAATCT GGACAAAGTG CTGTCCGCCT ACAACAAGCA 7141 CCGGGATAAG CCCATCAGAG AGCAGGCCGA GAATATCATC CACCTGTTTA CCCTGACCAA 7201 TCTGGGAGCC CCTGCCGCCT TCAAGTACTT TGACACCACC ATCGACCGGA AGAGGTACAC 7261 CAGCACCAAA GAGGTGCTGG ACGCCACCCT GATCCACCAG AGCATCACCG GCCTGTACGA 7321 GACACGGATC GACCTGTCTC AGCTGGGAGG CGACAAGCGA CCTGCCGCCA CAAAGAAGGC 7381 TGGACAGGCT AAGAAGAAGA AAGATTACAA AGACGATGAC GATAAGGGAT CCGGCGCAAC 7441 AAACTTCTCT CTGCTGAAAC AAGCCGGAGA TGTCGAAGAG AATCCTGGAC CGACCGAGTA 7501 CAAGCCCACG GTGCGCCTCG CCACCCGCGA CGACGTCCCC AGGGCCGTAC GCACCCTCGC 7561 CGCCGCGTTC GCCGACTACC CCGCCACGCG CCACACCGTC GATCCGGACC GCCACATCGA 7621 GCGGGTCACC GAGCTGCAAG AACTCTTCCT CACGCGCGTC GGGCTCGACA TCGGCAAGGT 7681 GTGGGTCGCG GACGACGGCG CCGCGGTGGC GGTCTGGACC ACGCCGGAGA GCGTCGAAGC 7741 GGGGGCGGTG TTCGCCGAGA TCGGCCCGCG CATGGCCGAG TTGAGCGGTT CCCGGCTGGC 7801 CGCGCAGCAA CAGATGGAAG GCCTCCTGGC GCCGCACCGG CCCAAGGAGC CCGCGTGGTT 7861 CCTGGCCACC GTCGGAGTCT CGCCCGACCA CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT 7921 GCTCCCCGGA GTGGAGGCGG CCGAGCGCGC CGGGGTGCCC GCCTTCCTGG AGACCTCCGC 7981 GCCCCGCAAC CTCCCCTTCT ACGAGCGGCT CGGCTTCACC GTCACCGCCG ACGTCGAGGT 8041 GCCCGAAGGA CCGCGCACCT GGTGCATGAC CCGCAAGCCC GGTGCCTGAA CGCGTTAAGT 8101 CGACAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT 8161 TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC 8221 CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA 8281 GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC 8341 CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT 8401 CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG 8461 GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAA TCATCGTCCT TTCCTTGGCT 8521 GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC 8581 CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG 8641 TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCGTCGACT 8701 TTAAGACCAA TGACTTACAA GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG 8761 GGACTGGAAG GGCTAATTCA CTCCCAACGA AGACAAGATC TGCTTTTTGC TTGTACTGGG 8821 TCTCTCTGGT TAGACCAGAT CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG 8881 CTTAAGCCTC AATAAAGCTT GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT 8941 GACTCTGGTA ACTAGAGATC CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGg 9001 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt 9061 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 9121 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg 9181 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc 9241 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca 9301 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 9361 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 9421 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag 9481 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 9541 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 9601 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 9661 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 9721 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 9781 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 9841 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 9901 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 9961 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag 10021 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg 10081 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat 10141 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca 10201 gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc 10261 ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg 10321 tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg 10381 tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact 10441 tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt 10501 tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac 10561 acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg 10621 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg 10681 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 10741 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 10801 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt 10861 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 10921 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 10981 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 11041 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 11101 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 11161 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 11221 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 11281 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 11341 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 11401 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 11461 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 11521 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 11581 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 11641 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 11701 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 11761 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 11821 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 11881 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 11941 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 12001 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 12061 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 12121 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 12181 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 12241 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 12301 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 12361 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 12421 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 12481 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 12541 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 12601 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 12661 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 12721 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 12781 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 12841 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 12901 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 12961 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gac

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.