pLV3-U6-GTF2F1-shRNA2-CopGFP-Puro plasmid

PVT96953

pLV3-U6-GTF2F1-shRNA2-CopGFP-Puro plasmid Information

Plasmid name: pLV3-U6-GTF2F1(human)-shRNA2-CopGFP-Puro
Alias: CGAGTTCCTAAGAATACAA
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-GTF2F1-shRNA2-CopGFP-Puro plasmid Description

pLV3-U6-GTF2F1-shRNA2-CopGFP-Puro This is a plasmid suitable for interference silencing in mammalian cells, and it can also be used for lentivirus packaging.

pLV3-U6-GTF2F1-shRNA2-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtGAGGGCCT ATTTCCCATG ATTCCTTCAT ATTTGCATAT ACGATACAAG 1981 GCTGTTAGAG AGATAATTAG AATTAATTTG ACTGTAAACA CAAAGATATT AGTACAAAAT 2041 ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG TTTTAAAATT ATGTTTTAAA 2101 ATGGACTATC ATATGCTTAC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC 2161 TTGTGGAAAG GACTCATCgc tagcgaattc gCGAGTTCCT AAGAATACAA ttcaagagaT 2221 TGTATTCTTA GGAACTCGtt ttttggatcc gcggccgcGa aggatctgcg atcgctccgg 2281 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 2341 cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 2401 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 2461 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc 2521 tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag 2581 tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag 2641 tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact 2701 cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt 2761 ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat 2821 ggagagcgac gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct 2881 gaacggcgtg gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat 2941 gaccaacaag atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca 3001 cgtgatgggc tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt 3061 cctgcacgcc atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg 3121 cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt 3181 caaggtggtg ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg 3241 cagcaacgcc accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt 3301 cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat 3361 gcacttcaag agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt 3421 ccgccgcgtg gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc 3481 cttcaagacc cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga 3541 cggcaccgcc ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg 3601 cggtgacgtg gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc 3661 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc 3721 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga 3781 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc 3841 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat 3901 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg 3961 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc 4021 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc 4081 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta 4141 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg 4201 gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag 4261 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat 4321 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc 4381 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg 4441 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct 4501 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct 4561 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg 4621 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac 4681 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct 4741 gccggctctg cggcctcttc cgcgtctccg ccttcgccct cagacgagtc ggatctccct 4801 ttggccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct 4861 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaaata 4921 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct 4981 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 5041 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 5101 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 5161 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg 5221 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 5281 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg 5341 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 5401 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 5461 tttggaggcc tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt 5521 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 5581 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 5641 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 5701 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 5761 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 5821 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 5881 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 5941 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 6001 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 6061 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 6121 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 6181 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 6241 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 6301 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 6361 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 6421 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 6481 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 6541 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 6601 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 6661 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 6721 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 6781 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 6841 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 6901 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 6961 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 7021 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 7081 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 7141 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 7201 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 7261 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 7321 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 7381 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 7441 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 7501 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 7561 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 7621 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt 7681 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc 7741 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg 7801 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt 7861 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg 7921 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc 7981 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 8041 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 8101 cgacgttgta aaacgacggc cagtgccaag ctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???