pLV3-U6-Mapk8-shRNA1-U6-Mapk9-shRNA1-CopGFP-Puro plasmid

PVT103257

pLV3-U6-Mapk8-shRNA1-U6-Mapk9-shRNA1-CopGFP-Puro plasmid Information

Plasmid name: pLV3-U6-Mapk8(mouse)-shRNA1-U6-Mapk9(mouse)-shRNA1-CopGFP-Puro
Alias: Mapk8 GCAAATCTTTGCCAAGTGATT Mapk9CTCAACTTTCACTGTTCTAAA
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: EF1a-R
Copy number: High
Induction:

pLV3-U6-Mapk8-shRNA1-U6-Mapk9-shRNA1-CopGFP-Puro plasmid Description

pLV3-U6-Mapk8-shRNA1-U6-Mapk9-shRNA1-CopGFP-Puro This is a plasmid suitable for interference silencing in mammalian cells, and it can also be used for lentivirus packaging.

pLV3-U6-Mapk8-shRNA1-U6-Mapk9-shRNA1-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct 241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 421 ccgaacaggg acttgaaagc gaaagggaaa ccagagGAGc tctctcgacg caggactcgg 481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt 541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg 601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa 661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg 721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca 781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa 841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa 901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag 961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt 1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg 1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac 1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct 1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct 1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg 1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa 1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa 1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa 1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac 1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag 1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc 1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga 1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtATCG 1801 GTtaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga 1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat 1921 tttatcgata ctagtGAGGG CCTATTTCCC ATGATTCCTT CATATTTGCA TATACGATAC 1981 AAGGCTGTTA GAGAGATAAT TAGAATTAAT TTGACTGTAA ACACAAAGAT ATTAGTACAA 2041 AATACGTGAC GTAGAAAGTA ATAATTTCTT GGGTAGTTTG CAGTTTTAAA ATTATGTTTT 2101 AAAATGGACT ATCATATGCT TACCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT 2161 ATCTTGTGGA AAGGACTCAT Cgctagcgaa ttGGCAAATC TTTGCCAAGT GATTCTCGAG 2221 AATCACTTGG CAAAGATTTG CTTTTTTgag cggccgcccc cttcaccgag ggcctatttc 2281 ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata attggaatta 2341 atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc 2401 ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa 2461 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga aacACCGGCT 2521 CAACTTTCAC TGTTCTAAAT TCAAGAGATT TAGAACAGTG AAAGTTGAGT TTTTTggatc 2581 cgcggccgcG aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc 2641 ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt 2701 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg 2761 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg 2821 ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc 2881 ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg 2941 cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt 3001 gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc 3061 tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc 3121 tgtgaccggc gcctacgcta gacgccacca tggagagcga cgagagcggc ctgcccgcca 3181 tggagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag ctggtgggcg 3241 gcggagaggg cacccccaag cagggccgca tgaccaacaa gatgaagagc accaaaggcg 3301 ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc taccacttcg 3361 gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac ggcggctaca 3421 ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc 3481 gctacgaggc cggccgcgtg atcggcgact tcaaggtggt gggcaccggc ttccccgagg 3541 acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag cacctgcacc 3601 ccatgggcga taacgtgctg gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg 3661 gctactacag cttcgtggtg gacagccaca tgcacttcaa gagcgccatc caccccagca 3721 tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggagctg cacagcaaca 3781 ccgagctggg catcgtggag taccagcacg ccttcaagac ccccatcgcc ttcgccagat 3841 cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc cggacccggc tccaccggat 3901 ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta 3961 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac 4021 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc 4081 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca 4141 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga 4201 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt 4261 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc 4321 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca 4381 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg 4441 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg 4501 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa 4561 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 4621 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 4681 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 4741 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 4801 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 4861 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 4921 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg 4981 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 5041 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtctcc 5101 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta 5161 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 5221 ctggaagggc taattcactc ccaacgaaga taagatctgc tttttgcttg tactgggtct 5281 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 5341 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 5401 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 5461 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 5521 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 5581 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 5641 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgCCCATC CCGCCCCTAA 5701 CTCCGcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 5761 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 5821 gcctagactt ttgcagagac caaattcgta atcatgtcat agctgtttcc tgtgtgaaat 5881 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg 5941 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag 6001 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 6061 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 6121 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 6181 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 6241 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 6301 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 6361 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 6421 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 6481 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 6541 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 6601 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 6661 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct 6721 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 6781 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 6841 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 6901 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 6961 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 7021 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 7081 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 7141 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 7201 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 7261 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 7321 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 7381 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 7441 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 7501 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 7561 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 7621 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 7681 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 7741 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 7801 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 7861 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 7921 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 7981 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta 8041 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt 8101 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc 8161 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt 8221 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg 8281 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac 8341 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga 8401 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa 8461 acgacggcca gtgccaagct g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???