pLV3-U6-Mettl14-shRNA1-CopGFP-Puro plasmid

PVT90081

pLV3-U6-Mettl14-shRNA1-CopGFP-Puro plasmid Information

Plasmid name: pLV3-U6-Mettl14(rat)-shRNA1-CopGFP-Puro
Alias: ATAGCATTGGTGCTGTGTTAA
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Rat
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-Mettl14-shRNA1-CopGFP-Puro plasmid Description

pLV3-U6-Mettl14-shRNA1-CopGFP-Puro This is a plasmid suitable for interference silencing in mammalian cells, and it can also be used for lentivirus packaging.

pLV3-U6-Mettl14-shRNA1-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtGAGGGCCT ATTTCCCATG ATTCCTTCAT ATTTGCATAT ACGATACAAG 1981 GCTGTTAGAG AGATAATTAG AATTAATTTG ACTGTAAACA CAAAGATATT AGTACAAAAT 2041 ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG TTTTAAAATT ATGTTTTAAA 2101 ATGGACTATC ATATGCTTAC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC 2161 TTGTGGAAAG GACTCATCgc tagcgaattc GATAGCATTG GTGCTGTGTT AAttcaagag 2221 aTTAACACAG CACCAATGCT ATTTTTTTgg atccgcggcc gcGaaggatc tgcgatcgct 2281 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 2341 gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 2401 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 2461 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag 2521 gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt 2581 tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg 2641 taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta 2701 gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt 2761 cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg ctagacgcca 2821 ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc atcaccggca 2881 ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc aagcagggcc 2941 gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc tacctgctga 3001 gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc tacgagaacc 3061 ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag aagtacgagg 3121 acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc gtgatcggcg 3181 acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc gacaagatca 3241 tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg ctggtgggca 3301 gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc 3361 acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc cccatgttcg 3421 ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg gagtaccagc 3481 acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg 3541 tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga agtcttctaa 3601 catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc acggtgcgcc 3661 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact 3721 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc 3781 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg 3841 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg 3901 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg 3961 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg 4021 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg 4081 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct 4141 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca 4201 cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac aaaatttgtg 4261 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt 4321 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata 4381 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 4441 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 4501 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct 4561 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt 4621 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg 4681 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 4741 tgctgccggc tctgcggcct cttccgcgtc tccgccttcg ccctcagacg agtcggatct 4801 ccctttggcc gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag 4861 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa 4921 aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg 4981 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc 5041 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct 5101 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt 5161 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata 5221 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 5281 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat 5341 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 5401 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg 5461 cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat ggtcatagct 5521 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 5581 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 5641 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 5701 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 5761 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 5821 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 5881 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 5941 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 6001 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 6061 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 6121 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 6181 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 6241 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 6301 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 6361 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 6421 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 6481 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 6541 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 6601 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 6661 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 6721 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 6781 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 6841 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 6901 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 6961 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 7021 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 7081 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 7141 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 7201 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 7261 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 7321 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 7381 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 7441 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 7501 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 7561 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 7621 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 7681 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg 7741 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 7801 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 7861 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 7921 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc 7981 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca 8041 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca 8101 gtcacgacgt tgtaaaacga cggccagtgc caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.