pLV3-U6-NFE2L2-shRNA2-Puro plasmid

PVT94186

pLV3-U6-NFE2L2-shRNA2-Puro plasmid Information

Plasmid name: pLV3-U6-NFE2L2(human)-shRNA2-Puro
Alias: GACAGAAGTTGACAATTAT
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-NFE2L2-shRNA2-Puro plasmid Description

pLV3-U6-NFE2L2-shRNA2-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-U6-NFE2L2-shRNA2-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tCGGGTTTAT TACAGGGACA GCAGAGATCC AGTTTATCGA TCTGGGCAGG AAGAGGGCCT 1981 ATTTCCCATG ATTCCTTCAT ATTTGCATAT ACGATACAAG GCTGTTAGAG AGATAATTAG 2041 AATTAATTTG ACTGTAAACA CAAAGATATT AGTACAAAAT ACGTGACGTA GAAAGTAATA 2101 ATTTCTTGGG TAGTTTGCAG TTTTAAAATT ATGTTTTAAA ATGGACTATC ATATGCTTAC 2161 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGGATC 2221 CGGACAGAAG TTGACAATTA Tttcaagaga ATAATTGTCA ACTTCTGTCT TTTTTGAATT 2281 Cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc 2341 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg 2401 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa 2461 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga 2521 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga 2581 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga 2641 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg 2701 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct 2761 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg 2821 gcgcctacgc tagatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt 2881 cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac 2941 cgtcgacccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg 3001 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg 3061 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc 3121 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca 3181 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg 3241 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt 3301 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt 3361 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa 3421 gcccggtgcc tgagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg 3481 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta 3541 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct 3601 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt 3661 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac 3721 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg 3781 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc 3841 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg 3901 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct 3961 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc 4021 ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact 4081 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaaa taagatctgc 4141 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct 4201 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt 4261 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt 4321 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa 4381 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat 4441 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 4501 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac 4561 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 4621 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 4681 cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg 4741 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 4801 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 4861 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 4921 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 4981 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 5041 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 5101 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 5161 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 5221 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 5281 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 5341 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 5401 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 5461 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 5521 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 5581 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 5641 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 5701 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 5761 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 5821 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 5881 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 5941 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 6001 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 6061 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 6121 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 6181 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 6241 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 6301 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 6361 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 6421 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 6481 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 6541 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 6601 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 6661 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 6721 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 6781 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 6841 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 6901 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga 6961 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga 7021 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg 7081 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat 7141 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg 7201 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 7261 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 7321 taaaacgacg gccagtgcca agctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???