pLV3-U6-PRC1-shRNA5-CopGFP-Puro plasmid

PVT105479

pLV3-U6-PRC1-shRNA5-CopGFP-Puro plasmid Information

Plasmid name: pLV3-U6-PRC1(human)-shRNA5-CopGFP-Puro
Alias: gctgagattgtgcggttaa
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F(Hairpin structure)
Reverse primer:
Copy number: High
Induction:

pLV3-U6-PRC1-shRNA5-CopGFP-Puro plasmid Description

pLV3-U6-PRC1-shRNA5-CopGFP-Puro It is a plasmid suitable for interference silencing in mammalian cells and also suitable for lentivirus packaging.

pLV3-U6-PRC1-shRNA5-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct 241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 421 ccgaacaggg acttgaaagc gaaagggaaa ccagagGAGc tctctcgacg caggactcgg 481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt 541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg 601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa 661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg 721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca 781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa 841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa 901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag 961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt 1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg 1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac 1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct 1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct 1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg 1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa 1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa 1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa 1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac 1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag 1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc 1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga 1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtATCG 1801 GTtaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga 1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat 1921 tttatcgata ctagtGAGGG CCTATTTCCC ATGATTCCTT CATATTTGCA TATACGATAC 1981 AAGGCTGTTA GAGAGATAAT TAGAATTAAT TTGACTGTAA ACACAAAGAT ATTAGTACAA 2041 AATACGTGAC GTAGAAAGTA ATAATTTCTT GGGTAGTTTG CAGTTTTAAA ATTATGTTTT 2101 AAAATGGACT ATCATATGCT TACCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT 2161 ATCTTGTGGA AAGGACTCAT Cgctagcgaa ttcggctgag attgtgcggt taattcaaga 2221 gattaaccgc acaatctcag cttttttgga tccgcggccg cGaaggatct gcgatcgctc 2281 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg 2341 ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt 2401 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt 2461 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg 2521 ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt 2581 gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt 2641 aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag 2701 actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc 2761 gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac 2821 catggagagc gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac 2881 cctgaacggc gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg 2941 catgaccaac aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag 3001 ccacgtgatg ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc 3061 cttcctgcac gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga 3121 cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga 3181 cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat 3241 ccgcagcaac gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag 3301 cttcgcccgc accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca 3361 catgcacttc aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc 3421 cttccgccgc gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca 3481 cgccttcaag acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt 3541 ggacggcacc gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac 3601 atgcggtgac gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct 3661 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta 3721 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca 3781 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg 3841 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga 3901 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga 3961 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt 4021 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc 4081 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt 4141 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac 4201 ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga 4261 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt 4321 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa 4381 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt 4441 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct 4501 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg 4561 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc 4621 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg 4681 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct 4741 gctgccggct ctgcggcctc ttccgcgtct ccgccttcgc cctcagacga gtcggatctc 4801 cctttgggcc gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag 4861 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa 4921 gataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg 4981 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc 5041 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct 5101 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt 5161 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata 5221 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 5281 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat 5341 cccgccccta actccgCCCA TCCCGCCCCT AACTCCGccc agttccgccc attctccgcc 5401 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 5461 attccagaag tagtgaggag gcttttttgg aggcctagac ttttgcagag accaaattcg 5521 taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 5581 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 5641 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 5701 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 5761 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 5821 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 5881 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 5941 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 6001 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 6061 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 6121 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 6181 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 6241 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 6301 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 6361 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 6421 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 6481 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 6541 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 6601 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 6661 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 6721 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 6781 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 6841 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 6901 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 6961 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 7021 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 7081 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 7141 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 7201 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 7261 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 7321 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 7381 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 7441 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 7501 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 7561 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 7621 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 7681 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 7741 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg 7801 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt 7861 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac 7921 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca 7981 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct 8041 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa 8101 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???