pLV3-U6-RAMP1-sgRNA4-EF1a-EGFP-Puro plasmid

PVT97986

pLV3-U6-RAMP1-sgRNA4-EF1a-EGFP-Puro plasmid Information

Plasmid name: pLV3-U6-RAMP1(human)-sgRNA4-EF1a-EGFP-Puro
Alias: ACCGTAGTTAGCCTCCTGGC
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Gene editing
Fragment type: CRISPR
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-RAMP1-sgRNA4-EF1a-EGFP-Puro plasmid Description

pLV3-U6-RAMP1-sgRNA4-EF1a-EGFP-Puro This is a plasmid suitable for gene editing in mammalian cells, and it can also be used for lentivirus packaging.

pLV3-U6-RAMP1-sgRNA4-EF1a-EGFP-Puro plasmid Sequence

1 cccgggtgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct 61 tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat 121 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgatccggt gcctagagaa 181 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg 241 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt 301 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg 361 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc 421 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt 481 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt 541 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat 601 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc 661 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca 721 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc 781 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg 841 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc 901 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac 961 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt 1021 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag 1081 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag 1141 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat 1201 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt 1261 cgtgacgtac ggccaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg 1321 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc 1381 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca 1441 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gccgtggcgg 1501 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca 1561 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc 1621 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggagtctcg cccgaccacc 1681 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg 1741 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg 1801 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc 1861 gcaagcccgg tgccGGATCC GGCGCAACAA ACTTCTCTCT GCTGAAACAA GCCGGAGATG 1921 TCGAAGAGAA TCCTGGACCG ATGGTGTCTA AGGGCGAAGA GCTGTTCACC GGGGTGGTGC 1981 CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG 2041 GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC 2101 TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC 2161 GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG 2221 TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA 2281 AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG 2341 ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA 2401 TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA GATCCGCCAC AACATCGAGG 2461 ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG 2521 TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG 2581 AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA 2641 TGGACGAGCT GTACAAGtaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat 2701 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 2761 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 2821 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 2881 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 2941 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 3001 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 3061 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 3121 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 3181 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 3241 gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag 3301 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga 3361 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc 3421 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt 3481 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc 3541 agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt 3601 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa 3661 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 3721 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc 3781 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 3841 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 3901 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata 3961 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc 4021 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata 4081 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg 4141 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 4201 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 4261 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 4321 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 4381 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 4441 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 4501 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 4561 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt 4621 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 4681 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 4741 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 4801 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 4861 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 4921 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg 4981 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 5041 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 5101 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 5161 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 5221 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc 5281 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 5341 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 5401 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 5461 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 5521 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 5581 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 5641 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 5701 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 5761 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 5821 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 5881 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa 5941 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 6001 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 6061 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 6121 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 6181 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 6241 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 6301 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 6361 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 6421 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg 6481 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg 6541 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc 6601 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc 6661 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata 6721 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca 6781 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag 6841 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt 6901 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg 6961 ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag 7021 tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc 7081 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa 7141 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag 7201 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac 7261 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg 7321 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg 7381 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt 7441 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc 7501 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac 7561 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata 7621 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt 7681 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg 7741 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat 7801 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg 7861 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca 7921 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct 7981 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg 8041 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac 8101 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact 8161 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg 8221 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt 8281 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg 8341 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata 8401 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat 8461 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga 8521 cccagagagg gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt 8581 agagagataa ttagaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga 8641 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac 8701 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttGTGG 8761 AAAGGACGAA ACACCgACCG TAGTTAGCCT CCTGGCgttt tagagctaGA AAtagcaagt 8821 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcTTTTTTaa 8881 gcttggcgta actagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa 8941 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata 9001 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg 9061 gacagcagag atccactttg gcgccggctc gaggggg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???