pLVX-EF1alpha-SARS-CoV-2-NSP12-2xStrep-IRES-Puro

PVT33197       2ug

pLVX-EF1alpha-SARS-CoV-2-NSP12-2xStrep-IRES-Puro Description

Alias:L-141378
Promoter:EF1a
Replicator:pUC
Clone strain:Stbl3
Culture conditions:37centigrade
Host:mammalian cells,Lentivirus
Use:Protein expression
Species:virus
Gene type:ORF
Prokaryotic resistance:Amp
Eukaryotic resistance:Puro
Fluorescent protein:

pLVX-EF1alpha-SARS-CoV-2-NSP12-2xStrep-IRES-Puro Sequence

tggaagggctaattcactcccaaagaagacaagatatccttgatctgtggatctaccacacacaaggctacttccctgat
tagcagaactacacaccagggccaggggtcagatatccactgacctttggatggtgctacaagctagtaccagttgagcc
agataaggtagaagaggccaataaaggagagaacaccagcttgttacaccctgtgagcctgcatgggatggatgacccgg
agagagaagtgttagagtggaggtttgacagccgcctagcatttcatcacgtggcccgagagctgcatccggagtacttc
aagaactgctgatatcgagcttgctacaagggactttccgctggggactttccagggaggcgtggcctgggcgggactgg
ggagtggcgagccctcagatcctgcatataagcagctgctttttgcctgtactgggtctctctggttagaccagatctga
gcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtg
tgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcg
cccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttgctgaagcgcgcacgg
caagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgag
agcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataa
attaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcagaaggct
gtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagatcattatataatacagtagca
accctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaa
aagtaagaccaccgcacagcaagcggccggccgctgatcttcagacctggaggaggagatatgagggacaattggagaag
tgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcaga
gagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtca
atgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggc
gcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaagatacctaa
aggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttgg
agtaataaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagctt
aatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattattggaattagataaatgggcaa
gtttgtggaattggtttaacataacaaattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggt
ttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacct
cccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatccattcgat
tagtgaacggatctcgacggtatcgcctttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtaga
cataatagcaacagacatacaaactaaagaattacaaaaacaaattacaaaaattcaaaattttcgggtttattacaggg
acagcagagatccagtttatcgatgagtaattcatacaaaaggactcgcccctgccttggggaatcccagggaccgtcgt
taaactcccactaacgtagaacccagagatcgctgcgttcccgccccctcacccgcccgctctcgtcatcactgaggtgg
agaagagcatgcgtgaggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggag
gggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctt
tttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccag
aacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttgaattactt
ccacgcccctggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgc
ttaaggagccccttcgcctcgtgcttgagttgaggcctggcttgggcgctggggccgccgcgtgcgaatctggtggcacc
ttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgctttttttctgg
caagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccg
tgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctg
gccggcctgctctggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcacca
gttgcgtgagcggaaagatggccgcttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcg
ggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccggg
cgccgtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggggttttatgcgatg
gagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgccct
ttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttccatttcaggtgtcgtgag
gatctatttccggtgaattcgccgccaccatgtcagcagacgcacaaagttttctgaaccgggtatgcggagtcagtgcg
gcgaggcttacgccctgtggtacgggaacatcaacggacgttgtttaccgcgccttcgacatatacaatgataaagtcgc
cggcttcgcaaagttccttaaaacgaattgctgtaggttccaagaaaaggacgaggatgataatttgatagactcctact
tcgtggtcaaacgacacacgttttcaaattatcagcacgaggaaacgatatataatcttcttaaagactgccctgcagtg
gcaaaacacgacttctttaagttccgcattgacggggatatggtcccccatatctcccgacagagattgacgaaatatac
aatggcggatctggtgtacgccctgagacacttcgatgagggcaattgtgatacactcaaggagattctcgtgacctaca
attgttgcgatgatgactatttcaataagaaagactggtacgacttcgtcgaaaaccctgatatattgagggtgtacgca
aacctgggtgaacgagtaagacaggcacttctgaaaaccgtccaattctgcgacgctatgagaaacgcgggcattgttgg
tgtgcttacgctggataaccaagacctcaatggtaattggtacgattttggggacttcatccaaaccactcctggctcag
gcgttcccgtggtagacagctactattctttgttgatgccaattcttacactcacaagggccctgaccgctgagtcacac
gtcgatacggacttgaccaagccctacatcaagtgggatcttctgaagtacgactttaccgaggaacgattgaagctttt
tgatcgctactttaaatactgggatcagacgtaccacccaaactgcgtgaattgcctggatgaccgatgtatactccatt
gcgcgaactttaacgttttgttctctacagtatttcctccgacgagcttcggaccgcttgtgagaaagatattcgtcgac
ggcgtcccatttgttgtcagcactggttaccactttcgcgagttgggggtggtccacaatcaggatgtcaatttgcattc
ctccaggctttcttttaaagagttgcttgtttatgccgctgaccccgcgatgcatgcagcctctgggaatcttttgctgg
acaaaaggacgacgtgctttagcgttgctgcccttacaaataacgtagcatttcaaaccgtaaaacccggtaattttaat
aaagatttttatgattttgcagtctctaagggttttttcaaggaggggagtagtgtagagcttaaacatttttttttcgc
tcaggatggtaacgctgcgatcagcgactacgactactatcgatacaatctcccgacgatgtgtgatatcaggcagttgt
tgttcgttgtagaagtggttgataagtattttgactgttatgacggcggctgtatcaatgccaaccaggtcattgtgaat
aacctcgataaatctgccgggttcccctttaacaagtgggggaaggcgcgactgtactacgacagcatgtcttacgaaga
tcaggacgcactctttgcatacacaaagaggaatgtgataccgactataactcaaatgaaccttaagtatgcaatcagcg
cgaaaaatcgggctaggactgttgccggagttagcatatgtagtaccatgacaaaccgacagttccaccagaagttgctg
aaatcaattgctgcgacccggggtgcgactgtagtaatcgggacatcaaagttctacggcgggtggcataacatgctcaa
gacggtctactcagacgttgagaatccgcatcttatgggttgggattatccaaagtgtgacagggcgatgcccaacatgt
tgcggataatggcctccttggtacttgcacggaaacatacaacctgctgtagtttgtcacaccgattttaccgactggca
aacgaatgtgctcaggttctcagtgaaatggtaatgtgtggcggctccctctacgttaagcccggtggcacaagctctgg
agacgcaactactgcctatgcaaacagtgtattcaacatctgccaggctgtcacggccaatgttaacgcattgctttcca
ctgacggaaataagattgcagacaaatacgtgagaaatttgcaacatagactctacgaatgcctctatcgcaatcgcgat
gttgatacggacttcgttaatgagttctatgcttacttgcgaaagcacttctccatgatgattctgtcagacgacgctgt
agtatgctttaattcaacgtatgcctcccagggtctggtcgcgtccataaaaaattttaaaagcgtcctgtattaccaaa
ataacgttttcatgagcgaagcgaaatgttggaccgagactgatcttactaagggccctcatgagttctgctcacaacac
actatgttggtcaagcagggcgacgattacgtgtatcttccttatcccgatcctagtaggattcttggagcaggttgctt
cgtggatgatatcgtcaaaacggacggcaccctcatgatagagaggttcgtctctttggctatagacgcgtatccgctta
ctaaacatcctaaccaagaatacgcggatgtattccatctttatctgcagtatatcagaaagttgcatgatgagctgacc
ggtcacatgttggacatgtatagcgtgatgcttaccaatgacaatacttcacggtactgggaacctgaattttacgaggc
gatgtatacgcctcacaccgtcctgcagctcgaaggcggcgggggatggagccatccacaattcgagaaaggcggtggtt
caggaggaggtagcgggggtggatcatggtcacatccgcagtttgaaaagtaaggatcccgcccctctccctcccccccc
cctaacgttactggccgaagccgcttggaataaggccggtgtgcgtttgtctatatgttattttccaccatattgccgtc
ttttggcaatgtgagggcccggaaacctggccctgtcttcttgacgagcattcctaggggtctttcccctctcgccaaag
gaatgcaaggtctgttgaatgtcgtgaaggaagcagttcctctggaagcttcttgaagacaaacaacgtctgtagcgacc
ctttgcaggcagcggaaccccccacctggcgacaggtgcctctgcggccaaaagccacgtgtataagatacacctgcaaa
ggcggcacaaccccagtgccacgttgtgagttggatagttgtggaaagagtcaaatggctctcctcaagcgtattcaaca
aggggctgaaggatgcccagaaggtaccccattgtatgggatctgatctggggcctcggtgcacatgctttacatgtgtt
tagtcgaggttaaaaaaacgtctaggccccccgaaccacggggacgtggttttcctttgaaaaacacgatgataagcttg
ccacaacccacaaggagacgaccttccatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtcccccg
ggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgacccggaccgccacatcgagc
gggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgcc
gcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagtt
gagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcc
tggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggcc
gagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgt
caccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctagacgcgtctggaac
aatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggata
cgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgc
tgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccact
ggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcat
cgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaagctga
cgtcctttccatggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctc
aatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgag
tcggatctccctttgggccgcctccccgcctggaattaattctgcagtcgagacctagaaaaacatggagcaatcacaag
tagcaatacagcagctaccaatgctgattgtgcctggctagaagcacaagaggaggaggaggtgggttttccagtcacac
ctcaggtacctttaagaccaatgacttacaaggcagctgtagatcttagccactttttaaaagaaaagaggggactggaa
gggctaattcactcccaacgaagacaagatatccttgatctgtggatctaccacacacaaggctacttccctgattagca
gaactacacaccagggccaggggtcagatatccactgacctttggatggtgctacaagctagtaccagttgagccagata
aggtagaagaggccaataaaggagagaacaccagcttgttacaccctgtgagcctgcatgggatggatgacccggagaga
gaagtgttagagtggaggtttgacagccgcctagcatttcatcacgtggcccgagagctgcatccggagtacttcaagaa
ctgctgatatcgagcttgctacaagggactttccgctggggactttccagggaggcgtggcctgggcgggactggggagt
ggcgagccctcagatcctgcatataagcagctgctttttgcctgtactgggtctctctggttagaccagatctgagcctg
ggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgccc
gtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttca
tgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggccttgacattgctagcgttt
accgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattc
cacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttg
cgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcgg
tttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcag
ctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaa
aaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcg
acgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgct
ctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctca
cgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccg
ctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggta
acaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaga
acagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaac
caccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttga
tcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatc
ttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagtta
ccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgt
agataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctcca
gatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtc
tattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggca
tcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatccccc
atgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcat
ggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaacca
agtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagc
agaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccag
ttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacag
gaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattat
tgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttcc
gcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatcaacttgtttattgcagcttataatggttac
aaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcat
caatgtatcttatcatgtctggatcaactggataactcaagctaaccaaaatcatcccaaacttcccaccccatacccta
ttaccactgccaattacctgtggtttcatttactctaaacctgtgattcctctgaattattttcattttaaagaaattgt
atttgttaaatatgtactacaaacttagtagt
//

Caution:
1.  This product is FOR RESEARCH USE ONLY!
2.  The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20ul ddH2O in to the tube of plasmid.