pMEL13 plasmid

PVT103211

pMEL13 plasmid Information

Plasmid name: pMEL13
Alias:
Gene lenght:
Host: Yeast
Use(s): Gene editing
Fragment type: CRISPR
Species: Saccharomyces cerevisiae
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: SNR52
Replicon: 2μ
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: KanR-F
Reverse primer:
Copy number: High
Induction:

pMEL13 plasmid Description

pMEL13 is a plasmid suitable for gene editing in yeast.

pMEL13 plasmid Sequence

1 ACAGGCAACA CGCAGATATA GGTGCGACGT GAACAGTGAG CTGTATGTGC GCAGCTCGCG 61 TTGCATTTTC GGAAGCGCTC GTTTTCGGAA ACGCTTTGAA GTTCCTATTC CGAAGTTCCT 121 ATTCTCTAGA AAGTATAGGA ACTTCAGAGC GCTTTTGAAA ACCAAAAGCG CTCTGAAGAC 181 GCACTTTCAA AAAACCAAAA ACGCACCGGA CTGTAACGAG CTACTAAAAT ATTGCGAATA 241 CCGCTTCCAC AAACATTGCT CAAAAGTATC TCTTTGCTAT ATATCTCTGT GCTATATCCC 301 TATATAACCT ACCCATCCAC CTTTCGCTCC TTGAACTTGC ATCTAAACTC GACCTCTACA 361 TTTTTTATGT TTATCTCTAG TATTACTCTT TAGACAAAAA AATTGTAGTA AGAACTATTC 421 ATAGAGTGAA TCGAAAACAA TACGAAAATG TAAACATTTC CTATACGTAG TATATAGAGA 481 CAAAATAGAA GAAACCGTTC ATAATTTTCT GACCAATGAA GAATCATCAA CGCTATCACT 541 TTCTGTTCAC AAAGTATGCG CAATCCACAT CGGTATAGAA TATAATCGGG GATGCCTTTA 601 TCTTGAAAAA ATGCACCCGC AGCTTCGCTA GTAATCAGTA AACGCGGGAA GTGGAGTCAG 661 GCTTTTTTTA TGGAAGAGAA AATAGACACC AAAGTAGCCT TCTTCTAACC TTAACGGACC 721 TACAGTGCAA AAAGTTATCA AGAGACTGCA TTATAGAGCG CACAAAGGAG AAAAAAAGTA 781 ATCTAAGATG CTTTGTTAGA AAAATAGCGC TCTCGGGATG CATTTTTGTA GAACAAAAAA 841 GAAGTATAGA TTCTTTGTTG GTAAAATAGC GCTCTCGCGT TGCATTTCTG TTCTGTAAAA 901 ATGCAGCTCA GATTCTTTGT TTGAAAAATT AGCGCTCTCG CGTTGCATTT TTGTTTTACA 961 AAAATGAAGC ACAGATTCTT CGTTGGTAAA ATAGCGCTTT CGCGTTGCAT TTCTGTTCTG 1021 TAAAAATGCA GCTCAGATTC TTTGTTTGAA AAATTAGCGC TCTCGCGTTG CATTTTTGTT 1081 CTACAAAATG AAGCACAGAT GCTTCGTTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA 1141 ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA 1201 CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT 1261 GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG 1321 CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG 1381 GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG 1441 AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG 1501 CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA 1561 GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG 1621 AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC 1681 GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG 1741 AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG 1801 TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC 1861 TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG 1921 TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG 1981 GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT 2041 ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA 2101 CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT 2161 AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG 2221 TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT 2281 TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT 2341 TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG 2401 CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT 2461 GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC 2521 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG 2581 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA 2641 CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG 2701 GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG 2761 GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA 2821 TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT 2881 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT 2941 GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA 3001 ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG 3061 CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG 3121 AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG 3181 GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT 3241 CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCGCG CAATTAACCC TCACTAAAGG 3301 GAACAAAAGC TGGAGCTTCT TTGAAAAGAT AATGTATGAT TATGCTTTCA CTCATATTTA 3361 TACAGAAACT TGATGTTTTC TTTCGAGTAT ATACAAGGTG ATTACATGTA CGTTTGAAGT 3421 ACAACTCTAG ATTTTGTAGT GCCCTCTTGG GCTAGCGGTA AAGGTGCGCA TTTTTTCACA 3481 CCCTACAATG TTCTGTTCAA AAGATTTTGG TCAAACGCTG TAGAAGTGAA AGTTGGTGCG 3541 CATGTTTCGG CGTTCGAAAC TTCTCCGCAG TGAAAGATAA ATGATCGATA CGTTCTCTAT 3601 GGAGGAGTTT TAGAGCTAGA AATAGCAAGT TAAAATAAGG CTAGTCCGTT ATCAACTTGA 3661 AAAAGTGGCA CCGAGTCGGT GGTGCTTTTT TTGTTTTTTA TGTCTTCGAG TCATGTAATT 3721 AGTTATGTCA CGCTTACGTT CACGCCCTCC CCCCACATCC GCTCTAACCG AAAAGGAAGG 3781 AGTTAGACAA CCTGAAGTCT AGGTCCCTAT TTATTTTTTT ATAGTTATGT TAGTATTAAG 3841 AACGTTATTT ATATTTCAAA TTTTTCTTTT TTTTCTGTAC AGACGCGTGT ACGCATGTAA 3901 CATTATACTG AAAACCTTGC TTGAGAAGGT TTTGGGACGC TCGAAGGCTT TAATTTGCGG 3961 CCGGTACCCA ATTCGCCCTA TAGTGAGTCG TATTACGCGC GCTCACTGGC CGTCGTTTTA 4021 CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC 4081 CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG 4141 CGCAGCCTGA ATGGCGAATG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG 4201 TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT 4261 TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC 4321 TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG 4381 GTGATGGTTC ACGTAGTGGG CCGTTGAACA TTCTTAGGCT GGTCGAATCA TTTAGACACG 4441 GGCATCGTCC TCTCGAAAGG TGGCATAGGC CACTAGTGGA TCTGATATCA CCTAATAACT 4501 TCGTATAGCA TACATTATAC GAAGTTATAT TAAGGGTTCT CGAGAGCTCG TTTTCGACAC 4561 TGGATGGCGG CGTTAGTATC GAATCGACAG CAGTATAGCG ACCAGCATTC ACATACGATT 4621 GACGCATGAT ATTACTTTCT GCGCACTTAA CTTCGCATCT GGGCAGATGA TGTCGAGGCG 4681 AAAAAAAATA TAAATCACGC TAACATTTGA TTAAAATAGA ACAACTACAA TATAAAAAAA 4741 CTATACAAAT GACAAGTTCT TGAAAACAAG AATCTTTTTA TTGTCAGTAC TGATTAGAAA 4801 AACTCATCGA GCATCAAATG AAACTGCAAT TTATTCATAT CAGGATTATC AATACCATAT 4861 TTTTGAAAAA GCCGTTTCTG TAATGAAGGA GAAAACTCAC CGAGGCAGTT CCATAGGATG 4921 GCAAGATCCT GGTATCGGTC TGCGATTCCG ACTCGTCCAA CATCAATACA ACCTATTAAT 4981 TTCCCCTCGT CAAAAATAAG GTTATCAAGT GAGAAATCAC CATGAGTGAC GACTGAATCC 5041 GGTGAGAATG GCAAAAGCTT ATGCATTTCT TTCCAGACTT GTTCAACAGG CCAGCCATTA 5101 CGCTCGTCAT CAAAATCACT CGCATCAACC AAACCGTTAT TCATTCGTGA TTGCGCCTGA 5161 GCGAGACGAA ATACGCGATC GCTGTTAAAA GGACAATTAC AAACAGGAAT CGAATGCAAC 5221 CGGCGCAGGA ACACTGCCAG CGCATCAACA ATATTTTCAC CTGAATCAGG ATATTCTTCT 5281 AATACCTGGA ATGCTGTTTT GCCGGGGATC GCAGTGGTGA GTAACCATGC ATCATCAGGA 5341 GTACGGATAA AATGCTTGAT GGTCGGAAGA GGCATAAATT CCGTCAGCCA GTTTAGTCTG 5401 ACCATCTCAT CTGTAACATC ATTGGCAACG CTACCTTTGC CATGTTTCAG AAACAACTCT 5461 GGCGCATCGG GCTTCCCATA CAATCGATAG ATTGTCGCAC CTGATTGCCC GACATTATCG 5521 CGAGCCCATT TATACCCATA TAAATCAGCA TCCATGTTGG AATTTAATCG CGGCCTCGAA 5581 ACGTGAGTCT TTTCCTTACC CATGGTTGTT TATGTTCGGA TGTGATGTGA GAACTGTATC 5641 CTAGCAAGAT TTTAAAAGGA AGTATATGAA AGAAGAACCT CAGTGGCAAA TCCTAACCTT 5701 TTATATTTCT CTACAGGGGC GCGGCGTGGG GACAATTCAA CGCGTCTGTG AGGGGAGCGT 5761 TTCCCTGCTC GCAGGTCTGC AGCGAGGAGC CGTAATTTTT GCTTCGCGCC GTGCGGCCAT 5821 CAAAATGTAT GGATGCAAAT GATTATACAT GGGGATGTAT GGGCTAAATG TACGGGCGAC 5881 AGTCACATCA TGCCCCTGAG CTGCGCACGT CAAGACTGTC AAGGAGGGTA TTCTGGGCCT 5941 CCATGTCGCT GGCCGGGTGA CCCGGCGGGG ACGAGGCAAG CTAAACAGAT CTCTAGACCT 6001 AATAACTTCG TATAGCATAC ATTATACGAA GTTATATTAA GGGTTGTCGA CCTGCAGCGT 6061 ACGAAGGTGC CTATTGATGA TCTGGCGGAA TGTCTGCCGT GCCATAGCCA TGCCTTCACA 6121 TATAGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???