pMESV-2×FLAG-DSC2-BSD plasmid

PVT98990

pMESV-2×FLAG-DSC2-BSD plasmid Information

Plasmid name: pMESV-2×FLAG-DSC2(human)-BSD
Alias: NM_001406506.1;ARVD11; CDHF2; DG2; DGII/III; DSC3
Gene lenght: 2277bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling:
Promoter: Non
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: PLXSN-F
Reverse primer:
Copy number: High
Induction:

pMESV-2×FLAG-DSC2-BSD plasmid Description

pMESV-2×FLAG-DSC2-BSD is a plasmid suitable for protein expression in mammalian cells.

pMESV-2×FLAG-DSC2-BSD plasmid Sequence

1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 361 tattagtcat cgctattacc atgggaggcg tggcctgggc gggactgggg agtggcgagc 421 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc 481 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 541 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 601 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga 661 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc 721 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg 781 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc 841 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat 901 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc 961 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga 1021 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat 1081 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa 1141 agcacagcaa gcagcaggat cttcagacct ggaaattccc tacaatcccc aaagtcaagg 1201 agtagtagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc 1261 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg 1321 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca 1381 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga 1441 cagcagaaat ccactttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt 1501 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag 1561 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta 1621 gaacatggaa aagtttagta aaacaccata aggaggagat atgagggaca attggagaag 1681 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1741 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1801 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1861 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1921 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1981 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 2041 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 2101 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 2161 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 2221 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 2281 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 2341 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 2401 cccaaccccg aggggaccga gctcaagctt cgaacgcgtt aacgggccca gcttcgataa 2461 aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt 2521 ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaatacat aactgagaat 2581 agagaagttc agatcaaggt taggaacaga gagacagcag aatatgggcc aaacaggata 2641 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg 2701 tcccgccctc agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg 2761 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc 2821 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct 2881 ccgatagact gcgtcgcccg ggtacccgta ttcccaataa agcctcttgc tgtttgcatc 2941 cgaatcgtgg actcgctgat ccttgggagg gtctcctcag attgattgac tgcccacctc 3001 gggggtcttt catttggagg ttccaccgag atttggagac ccctgcccag ggaccaccga 3061 cccccccgcc gggaggtaag ctggccagcg gtcgtttcgt gtctgtctct gtctttgtgc 3121 gtgtttgtgc cggcatctaa tgtttgcgcc tgcgtctgta ctagttagct aactagctct 3181 gtatctggcg gacccgtggt ggaactgacg agttctgaac acccggccgc aaccctggga 3241 gacgtcccag ggactttggg ggccgttttt gtggcccgac ctgaggaagg gagtcgatgt 3301 ggaatccgac cccgtcagga tatgtggttc tggtaggaga cgagaaccta aaacagttcc 3361 cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag ccgcgcgtct tgtctgctgc 3421 agcgctgcag catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa 3481 attagggcca gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg 3541 agcggatcgc tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct 3601 ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc 3661 tcatcaccca ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg 3721 tcccctacat cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct 3781 ttgtacaccc taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac 3841 ctcctcgttc gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg 3901 ccgggccacc atggactaca aagacgatga cgacaaggac tacaaagacg atgacgacaa 3961 ggaattcatg ctagaaaact ccttgggtcc ttttccactt ttccttcaac aggttcaatc 4021 tgacacggcc caaaactata ccatatacta ttccataaga ggtcctggag ttgaccaaga 4081 acctcggaat ttattttatg tggagagaga cactggaaac ttgtattgta ctcgtcctgt 4141 agatcgtgag cagtatgaat cttttgagat aattgccttt gcaacaactc cagatgggta 4201 tactccagaa cttccactgc ccctaataat caaaatagag gatgaaaatg ataactaccc 4261 aatttttaca gaagaaactt atacttttac aatttttgaa aattgcagag tgggcactac 4321 tgtgggacaa gtgtgtgcta ctgacaaaga tgagcctgac acgatgcaca cacgcctgaa 4381 gtactccatc attgggcagg tgccaccatc acccacccta ttttctatgc atccaactac 4441 aggcgtgatc accacaacat catctcagct agacagagag ttaattgaca agtaccagtt 4501 gaaaataaaa gtacaagaca tggatggtca gtattttggt ctacagacaa cttcaacttg 4561 tatcattaac attgatgatg taaatgacca cttgccaaca tttactcgta cttcttatgt 4621 gacatcagtg gaagaaaata cagttgatgt ggaaatctta cgagttactg ttgaggataa 4681 ggacttagtg aatactgcta actggagagc taattatacc attttaaagg gcaatgaaaa 4741 tggcaatttt aaaattgtaa cagatgccaa aaccaatgaa ggagttcttt gtgtagttaa 4801 gcctttgaat tatgaagaaa agcaacagat gatcttgcaa attggtgtag ttaatgaagc 4861 tccattttcc agagaggcta gtccaagatc agccatgagc acagcaacag ttactgttaa 4921 tgtagaagat caggatgagg gccctgagtg taaccctcca atacagactg ttcgcatgaa 4981 agaaaatgca gaagtgggaa caacaagcaa tggatataaa gcatatgacc cagaaacaag 5041 aagtagcagt ggcataaggt ataagaaatt aactgatcca acagggtggg tcaccattga 5101 tgaaaataca ggatcaatca aagttttcag aagcctggat agagaggcag agaccatcaa 5161 aaatggcata tataatatta cagtccttgc atcagaccaa ggagggagaa catgtacggg 5221 gacactgggc attatacttc aagacgtgaa tgataacagc ccattcatac ctaaaaagac 5281 agtgatcatc tgcaaaccca ccatgtcatc tgcggagatt gttgcggttg atcctgatga 5341 gcctatccat ggcccaccct ttgactttag tctggagagt tctacttcag aagtacagag 5401 aatgtggaga ctgaaagcaa ttaatgatac agcagcacgt ctttcctatc agaatgatcc 5461 tccatttggc tcatatgtag tacctataac agtgagagat agacttggca tgtctagtgt 5521 cacttcattg gatgttacac tgtgtgactg cattaccgaa aatgactgca cacatcgtgt 5581 agatccaagg attggcggtg gaggagtaca acttggaaag tgggccatcc ttgcaatatt 5641 gttgggcata gcattgctct tttgcatcct gtttacgctg gtctgtgggg cttctgggac 5701 gtctaaacaa ccaaaagtaa ttcctgatga tttagcccag cagaacctaa ttgtatcaaa 5761 cacagaagct cctggagatg acaaagtgta ttctgcgaat ggcttcacaa cccaaactgt 5821 gggcgcttct gctcagggag tttgtggcac cgtgggatca ggaatcaaaa acggaggtca 5881 ggagaccatc gaaatggtga aaggaggaca ccagacctcg gaatcctgcc ggggggctgg 5941 ccaccatcac accctggact cctgcagggg aggacacacg gaggtggaca actgcagata 6001 cacttactcg gagtggcaca gttttactca gccccgtctt ggtgaaaaag tgtatctgtg 6061 taatcaagat gaaaatcaca agcatgccca agactatgtc ctgacatata actatgaagg 6121 aagaggatcg gtggctgggt ctgtaggttg ttgcagtgaa cgacaagaag aagatgggct 6181 tgaatttttg gataatttgg agcccaaatt taggacacta gcagaagcat gcatgaagag 6241 atgacctagg gatccatgca gatctagacc tcgagcggga tcaattccgc ccccccccta 6301 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt 6361 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga 6421 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg 6481 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt 6541 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat 6601 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg 6661 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg 6721 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt 6781 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa 6841 cacgataata ccatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca 6901 acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc 6961 tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt 7021 gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc 7081 gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg 7141 cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg 7201 gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta aagcggccgc 7261 atcgatgccg tatacggtac ctttaagacc aatgacttac aaggcagctg tagatcttag 7321 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaaa gaagacaaga 7381 tctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc 7441 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttcagctgct cgagctagca 7501 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact 7561 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc 7621 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt 7681 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga 7741 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg 7801 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta 7861 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc 7921 atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct tggcgtaatc 7981 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 8041 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 8101 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat 8161 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg 8221 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 8281 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 8341 ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata 8401 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 8461 aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc 8521 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 8581 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 8641 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 8701 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 8761 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 8821 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 8881 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 8941 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 9001 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 9061 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 9121 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 9181 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 9241 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 9301 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 9361 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 9421 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 9481 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 9541 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 9601 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 9661 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 9721 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 9781 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 9841 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 9901 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 9961 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 10021 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 10081 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 10141 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 10201 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 10261 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 10321 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 10381 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 10441 aataggggtt ccgcgcacat ttccccgaaa agtgccacct g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???