pMSCV-Fbxo5-IRES-EGFP plasmid

PVT108635

pMSCV-Fbxo5-IRES-EGFP plasmid Information

Plasmid name: pMSCV-Fbxo5(mouse)-IRES-EGFP
Alias: NM_025995.3;2510044I10Rik; Emi1; Fbxo31
Gene lenght: 1266
Host: Mammalian cells,Retrovirus
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter:
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: PLXSN-F
Reverse primer:
Copy number: High
Induction:

pMSCV-Fbxo5-IRES-EGFP plasmid Description

pMSCV-Fbxo5-IRES-EGFP This is a plasmid suitable for protein expression in mammalian cells and can also be used for retrovirus packaging.

pMSCV-Fbxo5-IRES-EGFP plasmid Sequence

1 AATGAAAGAC CCCACCTGTA GGTTTGGCAA GCTAGCTTAA GTAACGCCAT TTTGCAAGGC 61 ATGGAAAATA CATAACTGAG AATAGAGAAG TTCAGATCAA GGTTAGGAAC AGAGAGACAG 121 CAGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC TCAGGGCCAA 181 GAACAGATGG TCCCCAGATG CGGTCCCGCC CTCAGCAGTT TCTAGAGAAC CATCAGATGT 241 TTCCAGGGTG CCCCAAGGAC CTGAAATGAC CCTGTGCCTT ATTTGAACTA ACCAATCAGT 301 TCGCTTCTCG CTTCTGTTCG CGCGCTTCTG CTCCCCGAGC TCAATAAAAG AGCCCACAAC 361 CCCTCACTCG GCGCGCCAGT CCTCCGATAG ACTGCGTCGC CCGGGTACCC GTATTCCCAA 421 TAAAGCCTCT TGCTGTTTGC ATCCGAATCG TGGACTCGCT GATCCTTGGG AGGGTCTCCT 481 CAGATTGATT GACTGCCCAC CTCGGGGGTC TTTCATTTGG AGGTTCCACC GAGATTTGGA 541 GACCCCTGCC CAGGGACCAC CGACCCCCCC GCCGGGAGGT AAGCTGGCCA GCGGTCGTTT 601 CGTGTCTGTC TCTGTCTTTG TGCGTGTTTG TGCCGGCATC TAATGTTTGC GCCTGCGTCT 661 GTACTAGTTA GCTAACTAGC TCTGTATCTG GCGGACCCGT GGTGGAACTG ACGAGTTCTG 721 AACACCCGGC CGCAACCCTG GGAGACGTCC CAGGGACTTT GGGGGCCGTT TTTGTGGCCC 781 GACCTGAGGA AGGGAGTCGA TGTGGAATCC GACCCCGTCA GGATATGTGG TTCTGGTAGG 841 AGACGAGAAC CTAAAACAGT TCCCGCCTCC GTCTGAATTT TTGCTTTCGG TTTGGAACCG 901 AAGCCGCGCG TCTTGTCTGC TGCAGCGCTG CAGCATCGTT CTGTGTTGTC TCTGTCTGAC 961 TGTGTTTCTG TATTTGTCTG AAAATTAGGG CCAGACTGTT ACCACTCCCT TAAGTTTGAC 1021 CTTAGGTCAC TGGAAAGATG TCGAGCGGAT CGCTCACAAC CAGTCGGTAG ATGTCAAGAA 1081 GAGACGTTGG GTTACCTTCT GCTCTGCAGA ATGGCCAACC TTTAACGTCG GATGGCCGCG 1141 AGACGGCACC TTTAACCGAG ACCTCATCAC CCAGGTTAAG ATCAAGGTCT TTTCACCTGG 1201 CCCGCATGGA CACCCAGACC AGGTCCCCTA CATCGTGACC TGGGAAGCCT TGGCTTTTGA 1261 CCCCCCTCCC TGGGTCAAGC CCTTTGTACA CCCTAAGCCT CCGCCTCCTC TTCCTCCATC 1321 CGCCCCGTCT CTCCCCCTTG AACCTCCTCG TTCGACCCCG CCTCGATCCT CCCTTTATCC 1381 AGCCCTCACT CCTTCTCTAG GCGCCGGAAT TCGCCACCat gagccggcgc acctgcagtg 1441 acctgcgccg gccgtcctcc tgtccctgcc gtcttggcgc gcggactacc gtggacggtt 1501 gtaaagaaga aagccctgtt ctttctgtta caatgaagtg ttttaattgc aaccctgatc 1561 tttccgagct tgaagtggtg aagcctgagg acagtgggat agaagcttcc tacagtcccg 1621 tgtgtttgga accttcctgt aatgactgtg ttagaaacca tgagaggttg tctttcatcg 1681 actcaccaat tgtgggacat gataacaagg aaaatcaacg cgtacaaaac acactagata 1741 gttcaaacga aacagaagag ctagaggcca gtagactgta tgaggacagt ggctactcat 1801 ctttcacaca aagtgaccgt gacgatggca tccttatcct ggagaatttc agaaacagtc 1861 cccaggcccg tctgctgcca tcacagagcc cggaccagca tcccaacaaa accttgctgc 1921 ctgtcctgca ttttgaaaga gtggtttgct caacactaaa aaagaatggc aagcgaaacc 1981 ctaaagtgga tcgagaaatg ctgaaggaag ttattgccag cgggaacttt agactgcaaa 2041 atataattgg caagaaaatg ggcctggagc acctagacat cctggctgag ctctcccgca 2101 ggggattcgt gcacctgttg gctaacattt tgactaagct cagcggcatg gacttagtaa 2161 atctgtctaa agtgagcaga atttggaaga agatactgga aaacaataag ggggcgttcc 2221 agctctacag caaaaccatg cagcgagtca ttgaaagcag taagttgtca ctacatgcta 2281 caacgagagg atatgttgtg ggcagagctg cactaacttg tgttcaaaag tcatcgacct 2341 gggcacctcc caaaaaagat gttcaaatca agtcctccag tcagcgtggt cagagagttt 2401 ctacctacag ccggcacaat gagttcgtgg aggtggcaaa gacattgaag aacaacgaaa 2461 gcctcaaagc ctgtgttcgc tgtaatttcc ctgcaaaata tgaccactat ttagagcgag 2521 cagtctgcaa acgggaaagc tgtcaatttg aatattgtac aaagtgtctg tgtgcttacc 2581 ataacaacaa agactgtttg aatggcaaga tcctaaaagc cagctgtaaa gtgggtcctt 2641 tgcctggaac taaaaagagt aaaaagaact tacaaagatt gtgaGGATCC CAATTGCTCG 2701 AGCGGGATCA ATTCCGCCCC CCCCCTAACG TTACTGGCCG AAGCCGCTTG GAATAAGGCC 2761 GGTGTGCGTT TGTCTATATG TTATTTTCCA CCATATTGCC GTCTTTTGGC AATGTGAGGG 2821 CCCGGAAACC TGGCCCTGTC TTCTTGACGA GCATTCCTAG GGGTCTTTCC CCTCTCGCCA 2881 AAGGAATGCA AGGTCTGTTG AATGTCGTGA AGGAAGCAGT TCCTCTGGAA GCTTCTTGAA 2941 GACAAACAAC GTCTGTAGCG ACCCTTTGCA GGCAGCGGAA CCCCCCACCT GGCGACAGGT 3001 GCCTCTGCGG CCAAAAGCCA CGTGTATAAG ATACACCTGC AAAGGCGGCA CAACCCCAGT 3061 GCCACGTTGT GAGTTGGATA GTTGTGGAAA GAGTCAAATG GCTCTCCTCA AGCGTATTCA 3121 ACAAGGGGCT GAAGGATGCC CAGAAGGTAC CCCATTGTAT GGGATCTGAT CTGGGGCCTC 3181 GGTGCACATG CTTTACATGT GTTTAGTCGA GGTTAAAAAA ACGTCTAGGC CCCCCGAACC 3241 ACGGGGACGT GGTTTTCCTT TGAAAAACAC GATAATACCA TGGTGAGCAA GGGCGAGGAG 3301 CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG 3361 TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC 3421 ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTAC 3481 GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC 3541 GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC 3601 AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG 3661 GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC 3721 AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT GAACTTCAAG 3781 ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC 3841 CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC 3901 CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC 3961 GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTAAA GCGGCCGCGA CTCTAGAGTC 4021 GACCTGCAGG CATGCAAGCT TCAGGTAGCC GGCTAACGTT AACAACCGGT ACCTCTAGAA 4081 CTATAGCTAG CATGCGCAAA TTTAAAGCGC TGATATCGAT AAAATAAAAG ATTTTATTTA 4141 GTCTCCAGAA AAAGGGGGGA ATGAAAGACC CCACCTGTAG GTTTGGCAAG CTAGCTTAAG 4201 TAACGCCATT TTGCAAGGCA TGGAAAATAC ATAACTGAGA ATAGAGAAGT TCAGATCAAG 4261 GTTAGGAACA GAGAGACAGC AGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC 4321 TGCCCCGGCT CAGGGCCAAG AACAGATGGT CCCCAGATGC GGTCCCGCCC TCAGCAGTTT 4381 CTAGAGAACC ATCAGATGTT TCCAGGGTGC CCCAAGGACC TGAAATGACC CTGTGCCTTA 4441 TTTGAACTAA CCAATCAGTT CGCTTCTCGC TTCTGTTCGC GCGCTTCTGC TCCCCGAGCT 4501 CAATAAAAGA GCCCACAACC CCTCACTCGG CGCGCCAGTC CTCCGATAGA CTGCGTCGCC 4561 CGGGTACCCG TGTATCCAAT AAACCCTCTT GCAGTTGCAT CCGACTTGTG GTCTCGCTGT 4621 TCCTTGGGAG GGTCTCCTCT GAGTGATTGA CTACCCGTCA GCGGGGGTCT TTCATGGGTA 4681 ACAGTTTCTT GAAGTTGGAG AACAACATTC TGAGGGTAGG AGTCGAATAT TAAGTAATCC 4741 TGACTCAATT AGCCACTGTT TTGAATCCAC ATACTCCAAT ACTCCTGAAA TAGTTCATTA 4801 TGGACAGCGC AGAAAGAGCT GGGGAGAATT GTGAAATTGT TATCCGCTCA CAATTCCACA 4861 CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT 4921 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT 4981 Gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctCTTCCGC 5041 TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA 5101 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 5161 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 5221 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 5281 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 5341 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 5401 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 5461 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 5521 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 5581 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 5641 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 5701 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 5761 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 5821 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 5881 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 5941 CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC 6001 TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT 6061 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC 6121 ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG 6181 AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG 6241 AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT 6301 GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG 6361 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT 6421 TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC 6481 TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC 6541 ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA 6601 TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG 6661 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC 6721 CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG 6781 GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT 6841 CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT 6901 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC 6961 ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC 7021 GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT 7081 CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG 7141 CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT 7201 TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA 7261 CCGCATCAGG CGCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG 7321 GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG 7381 GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCGCAAG GAATGGTGCA 7441 TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG 7501 AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG 7561 ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG 7621 TAGAGGCGAT TAGTCCAATT TGTTAAAGAC AGGATATCAG TGGTCCAGGC TCTAGTTTTG 7681 ACTCAACAAT ATCACCAGCT GAAGCCTATA GAGTACGAGC CATAGATAAA ATAAAAGATT 7741 TTATTTAGTC TCCAGAAAAA GGGGGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.