pMSGV1-DUSP4-PGK-Puro plasmid

PVT110644

pMSGV1-DUSP4-PGK-Puro plasmid Information

Plasmid name: pMSGV1-DUSP4(human)-PGK-Puro
Alias: NM_001394.7;HVH2; MKP-2; MKP2; TYP
Gene lenght: 1185
Host: Mammalian cells,Retrovirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter:
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: PLXSN-F
Reverse primer:
Copy number: High
Induction:

pMSGV1-DUSP4-PGK-Puro plasmid Description

pMSGV1-DUSP4-PGK-Puro Plasmid suitable for gene expression in mammalian cells, also suitable for retrovirus packaging.

pMSGV1-DUSP4-PGK-Puro plasmid Sequence

1 NAATGAAAGA CCCCACCTGT AGGTTTGGCA AGCTAGCTTA AGTAACGCCA TTTTGCAAGG 61 CATGGAAAAT ACATAACTGA GAATAGAGAA GTTCAGATCA AGGTTAGGAA CAGAGAGACA 121 GCAGAATATG GGCCAAACAG GATATCTGTG GTAAGCAGTT CCTGCCCCGG CTCAGGGCCA 181 AGAACAGATG GTCCCCAGAT GCGGTCCCGC CCTCAGCAGT TTCTAGAGAA CCATCAGATG 241 TTTCCAGGGT GCCCCAAGGA CCTGAAATGA CCCTGTGCCT TATTTGAACT AACCAATCAG 301 TTCGCTTCTC GCTTCTGTTC GCGCGCTTCT GCTCCCCGAG CTCAATAAAA GAGCCCACAA 361 CCCCTCACTC GGCGCGCCAG TCCTCCGATA GACTGCGTCG CCCGGGTACC CGTATTCCCA 421 ATAAAGCCTC TTGCTGTTTG CATCCGAATC GTGGACTCGC TGATCCTTGG GAGGGTCTCC 481 TCAGATTGAT TGACTGCCCA CCTCGGGGGT CTTTCATTTG GAGGTTCCAC CGAGATTTGG 541 AGACCCCTGC CCAGGGACCA CCGACCCCCC CGCCGGGAGG TAAGCTGGCC AGCGGTCGTT 601 TCGTGTCTGT CTCTGTCTTT GTGCGTGTTT GTGCCGGCAT CTAATGTTTG CGCCTGCGTC 661 TGTACTAGTT AGCTAACTAG CTCTGTATCT GGCGGACCCG TGGTGGAACT GACGAGTTCG 721 GAACACCCGG CCGCAACCCT GGGAGACGTC CCAGGGACTT CGGGGGCCGT TTTTGTGGCC 781 CGACCTGAGT CCTAAAATCC CGATCGTTTA GGACTCTTTG GTGCACCCCC CTTAGAGGAG 841 GGATATGTGG TTCTGGTAGG AGACGAGAAC CTAAAACAGT TCCCGCCTCC GTCTGAATTT 901 TTGCTTTCGG TTTGGGACCG AAGCCGCGCC GCGCGTCTTG TCTGCTGCAG CATCGTTCTG 961 TGTTGTCTCT GTCTGACTGT GTTTCTGTAT TTGTCTGAAA ATATGGGCCC GGGCTAGCCT 1021 GTTACCACTC CCTTAAGTTT GACCTTAGGT CACTGGAAAG ATGTCGAGCG GATCGCTCAC 1081 AACCAGTCGG TAGATGTCAA GAAGAGACGT TGGGTTACCT TCTGCTCTGC AGAATGGCCA 1141 ACCTTTAACG TCGGATGGCC GCGAGACGGC ACCTTTAACC GAGACCTCAT CACCCAGGTT 1201 AAGATCAAGG TCTTTTCACC TGGCCCGCAT GGACACCCAG ACCAGGTCCC CTACATCGTG 1261 ACCTGGGAAG CCTTGGCTTT TGACCCCCCT CCCTGGGTCA AGCCCTTTGT ACACCCTAAG 1321 CCTCCGCCTC CTCTTCCTCC ATCCGCCCCG TCTCTCCCCC TTGAACCTCC TCGTTCGACC 1381 CCGCCTCGAT CCTCCCTTTA TCCAGCCCTC ACTCCTTCTC TAGGCGCCCC CATATGGCCA 1441 TATGAGATCT TATATGGGGC ACCCCCGCCC CTTGTAAACT TCCCTGACCC TGACATGACA 1501 AGAGTTACTA ACAGCCCCTC TCTCCAAGCT CACTTACAGG CTCTCTACTT AGTCCAGCAC 1561 GAAGTCTGGA GACCTCTGGC GGCAGCCTAC CAAGAACAAC TGGACCGACC GGTGGTACCT 1621 CACCCTTACC GAGTCGGCGA CACAGTGTGG GTCCGCCGAC ACCAGACTAA GAACCTAGAA 1681 CCTCGCTGGA AAGGACCTTA CACAGTCCTG CTGACCACCC CCACCGCCCT CAAAGTAGAC 1741 GGCATCGCAG CTTGGATACA CGCCGCCCAC GTGAAGGCTG CCGACCCCGG GGGTGGACCA 1801 TCCTCTAGCC CTCGAGAAGC TTGCCACCat ggtgacgatg gaggagctgc gggagatgga 1861 ctgcagtgtg ctcaaaaggc tgatgaaccg ggacgagaat ggcggcggcg cgggcggcag 1921 cggcagccac ggcaccctgg ggctgccgag cggcggcaag tgcctgctgc tggactgcag 1981 accgttcctg gcgcacagcg cgggctacat cctaggttcg gtcaacgtgc gctgtaacac 2041 catcgtgcgg cggcgggcta agggctccgt gagcctggag cagatcctgc ccgccgagga 2101 ggaggtacgc gcccgcttgc gctccggcct ctactcggcg gtcatcgtct acgacgagcg 2161 cagcccgcgc gccgagagcc tccgcgagga cagcaccgtg tcgctggtgg tgcaggcgct 2221 gcgccgcaac gccgagcgca ccgacatctg cctgctcaaa ggcggctatg agaggttttc 2281 ctccgagtac ccagaattct gttctaaaac caaggccctg gcagccatcc cacccccggt 2341 tccccccagt gccacagagc ccttggacct gggctgcagc tcctgtggga ccccactaca 2401 cgaccagggg ggtcctgtgg agatccttcc cttcctctac ctcggcagtg cctaccatgc 2461 tgcccggaga gacatgctgg acgccctggg catcacggct ctgttgaatg tctcctcgga 2521 ctgcccaaac cactttgaag gacactatca gtacaagtgc atcccagtgg aagataacca 2581 caaggccgac atcagctcct ggttcatgga agccatagag tacatcgatg ccgtgaagga 2641 ctgccgtggg cgcgtgctgg tgcactgcca ggcgggcatc tcgcggtcgg ccaccatctg 2701 cctggcctac ctgatgatga agaaacgggt gaggctggag gaggccttcg agttcgttaa 2761 gcagcgccgc agcatcatct cgcccaactt cagcttcatg gggcagctgc tgcagttcga 2821 gtcccaggtg ctggccacgt cctgtgctgc ggaggctgct agcccctcgg gacccctgcg 2881 ggagcggggc aagacccccg ccacccccac ctcgcagttc gtcttcagct ttccggtctc 2941 cgtgggcgtg cactcggccc ccagcagcct gccctacctg cacagcccca tcaccacctc 3001 tcccagctgt tagtctagat aattctaccg ggtaggggag gcgcttttcc caaggcagtc 3061 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc 3121 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt 3181 cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc 3241 gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac 3301 cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt 3361 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct 3421 caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa 3481 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc 3541 aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc 3601 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc 3661 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc 3721 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc 3781 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag 3841 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg 3901 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag 3961 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc 4021 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc 4081 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc 4141 ggtgcctgac cgcgtctggG AATTCTGCAG ATATCCAGCA CAGTGGCGGC CGCtagcGTC 4201 GACGGTACCG CGGGCCCGGG ATCCGATAAA ATAAAAGATT TTATTTAGTC TCCAGAAAAA 4261 GGGGGGAATG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA GCTTAAGTAA CGCCATTTTG 4321 CAAGGCATGG AAAATACATA ACTGAGAATA GAGAAGTTCA GATCAAGGTT AGGAACAGAG 4381 AGACAGCAGA ATATGGGCCA AACAGGATAT CTGTGGTAAG CAGTTCCTGC CCCGGCTCAG 4441 GGCCAAGAAC AGATGGTCCC CAGATGCGGT CCCGCCCTCA GCAGTTTCTA GAGAACCATC 4501 AGATGTTTCC AGGGTGCCCC AAGGACCTGA AATGACCCTG TGCCTTATTT GAACTAACCA 4561 ATCAGTTCGC TTCTCGCTTC TGTTCGCGCG CTTCTGCTCC CCGAGCTCAA TAAAAGAGCC 4621 CACAACCCCT CACTCGGCGC GCCAGTCCTC CGATAGACTG CGTCGCCCGG GTACCCGTGT 4681 ATCCAATAAA CCCTCTTGCA GTTGCATCCG ACTTGTGGTC TCGCTGTTCC TTGGGAGGGT 4741 CTCCTCTGAG TGATTGACTA CCCGTCAGCG GGGGTCTTTC ATGGGTAACA GTTTCTTGAA 4801 GTTGGAGAAC AACATTCTGA GGGTAGGAGT CGAATATTAA GTAATCCTGA CTCAATTAGC 4861 CACTGTTTTG AATCCACATA CTCCAATACT CCTGAAATCC ATCGATGGAG TTCATTATGG 4921 ACAGCGCAGA AAGAGCTGGG GAGAATTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA 4981 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC 5041 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA 5101 TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC 5161 CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC 5221 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 5281 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 5341 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 5401 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 5461 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 5521 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 5581 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 5641 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 5701 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 5761 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 5821 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 5881 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 5941 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 6001 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 6061 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT 6121 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC 6181 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG 6241 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG 6301 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT 6361 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT 6421 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT 6481 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT 6541 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT 6601 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT 6661 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC 6721 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA 6781 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA 6841 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA 6901 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT 6961 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA 7021 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC 7081 TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG 7141 GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC 7201 GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC 7261 GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT 7321 ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG 7381 CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC 7441 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT 7501 AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCGCAAGGAA TGGTGCATGC 7561 AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA 7621 CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA 7681 TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG 7741 AGGCGATTAG TCCAATTTGT TAAAGACAGG ATATCAGTGG TCCAGGCTCT AGTTTTGACT 7801 CAACAATATC ACCAGCTGAA GCCTATAGAG TACGAGCCAT AGATAAAATA AAAGATTTTA 7861 TTTAGTCTCC AGAAAAAGGG GGG

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.