pmut-170-156-Fluc plasmid

PVT89930

pmut-170-156-Fluc plasmid Information

Plasmid name: pmut-170-156-Fluc
Alias: -
Gene lenght: 364bp
Host: Mammalian cells
Use(s):
Promoter detection
Fragment type: Promoter
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc
Promoter: mut-170-156
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: Fluc
Reverse primer:
Copy number: High
Induction:

pmut-170-156-Fluc plasmid Description

pmut-170-156-Fluc is a plasmid suitable for promoter detection in mammalian cells.

pmut-170-156-Fluc plasmid Sequence

1 CTCGAGCACT TGCTTTGTCC AGAGACTCGT CACACTGAAA CTATTTTCCG TTTGAGACCT 61 TCCAGGAGGA AAGCCATCCT CACACGCATG CAGTACAAAC CAGTGTCCTC CAGAGTCCGC 121 TGTGCCTACG GCCAGAGCAG CGACAGAGCC TTCCTCAAAC CTGTAGTGAC TGCCACACTT 181 TGCAAGGACA CCGTAGATTT TTACGTGAAT ATCTCCAACT TCCTCCCGAC GCAGCCTCTG 241 ATTGGCTCCT GGGCTTATAA GAAACGCGTG AATGAGCAGC TGCCGCGGGC AGAAAGTTGC 301 CGGAGGTCTC CGGGTGGTAT CGCCCTTTCC TCTTTGCCAG CCCGCTGGCG AGCCGAGCCA 361 GGGCAAGATG aagcttggca ttccggtact gttggtaaag ccaccatgga agacgccaaa 421 aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc tggagagcaa 481 ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt tacagatgca 541 catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa 601 gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag tgaaaactct 661 cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg 721 aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca gcctaccgtg 781 gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa gctcccaatc 841 atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca gtcgatgtac 901 acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt gccagagtcc 961 ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac tggtctgcct 1021 aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc cagagatcct 1081 atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc attccatcac 1141 ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt cgtcttaatg 1201 tatagatttg aagaagagct gtttctgagg agccttcagg attacaagat tcaaagtgcg 1261 ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga caaatacgat 1321 ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga agtcggggaa 1381 gcggttgcca agaggttcca tctgccaggt atcaggcaag gatatgggct cactgagact 1441 acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt cggtaaagtt 1501 gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct gggcgttaat 1561 caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt aaacaatccg 1621 gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga catagcttac 1681 tgggacgaag acgaacactt cttcatcgtt gaccgcctga agtctctgat taagtacaaa 1741 ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc caacatcttc 1801 gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg aacttcccgc cgccgttgtt 1861 gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt cgccagtcaa 1921 gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt accgaaaggt 1981 cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc caagaagggc 2041 ggaaagatcg ccgtgtaatt ctagagtcgg ggcggccggc cgcttcgagc agacatgata 2101 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt 2161 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt 2221 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt 2281 taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtc gaccgatgcc 2341 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc 2401 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt 2461 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 2521 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 2581 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 2641 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 2701 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 2761 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 2821 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 2881 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 2941 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 3001 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 3061 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 3121 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 3181 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 3241 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 3301 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 3361 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 3421 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 3481 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 3541 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 3601 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 3661 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 3721 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 3781 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 3841 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 3901 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 3961 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 4021 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 4081 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 4141 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 4201 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 4261 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 4321 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 4381 tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca 4441 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct 4501 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt 4561 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac 4621 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct 4681 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt 4741 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac 4801 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttcccat tcgccattca 4861 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagccca 4921 agctaccatg ataagtaagt aatattaagg tacgggaggt acttggagcg gccgcaataa 4981 aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagtactaac 5041 atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag 5101 tgcaagtgca ggtgccagaa catttctcta tcgata

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.