pNF-KB-TA-LUC
PVT1323 2ug
pNF-KB-TA-LUC Information
Promoter: HSV-TK
Replicator: pUC ori, F1 ori
Terminator: SV40 poly (A) signal
Plasmid classification: mammalian cells, signal pathway reporting vectors
Plasmid size: 4.9kb
Prokaryotic resistance: Amp
Cloned strain: DH5 alpha
Culture conditions: 37 centigrade, aerobic LB
Expression host: mammalian cells
Induction mode: no induction, instantaneous expression
5'sequencing primers: Sv40-polyA-R:GAAATTTGTGATGCTATTGC
3'sequencing primers: primers designed according to sequence
pNF-KB-TA-LUC Description
pNF-KB-TA-LUC is a lactation report plasmid.
pNF-KB-TA-LUC Sequence
LOCUS Exported 4902 bp ds-DNA circular SYN 13-MAR-2018
DEFINITION synthetic circular DNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4902)
FEATURES Location/Qualifiers
source 1..4902
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 34..73
/label=NFkB
promoter 86..117
/label=minP
/note="minimal TATA-box promoter with low basal activity"
regulatory 173..182
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 179..1831
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
polyA_signal 1872..1993
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(2412..3000)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3171..4031)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4032..4136)
/gene="bla"
/label=AmpR promoter
rep_origin 4163..4618
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
polyA_signal 4749..4797
/note="synthetic polyadenylation signal"
misc_feature 4811..4902
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
BASE COUNT 1275 a 1159 c 1181 g 1286 t
ORIGIN
1 atcgataggt accgagctct tacgcgtgct agcgggaatt tccgggaatt tccgggaatt
61 tccgggaatt tccagatcta gactctagag ggtatataat ggaagctcga attccagctt
121 ggcattccgg tactgttggt aaaaagcttg gcattccggt actgttggta aagccaccat
181 ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac
241 cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc
301 ttttacagat gcacatatcg aggtggacat cacttacgct gagtacttcg aaatgtccgt
361 tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg
421 cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc
481 agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta tgggcatttc
541 gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa
601 aaagctccca atcatccaaa aaattattat catggattct aaaacggatt accagggatt
661 tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt
721 tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact cctctggatc
781 tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga gattctcgca
841 tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt
901 tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg
961 agtcgtctta atgtatagat ttgaagaaga gctgtttctg aggagccttc aggattacaa
1021 gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa gcactctgat
1081 tgacaaatac gatttatcta atttacacga aattgcttct ggtggcgctc ccctctctaa
1141 ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg
1201 gctcactgag actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc
1261 ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac
1321 gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt cctatgatta tgtccggtta
1381 tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg
1441 agacatagct tactgggacg aagacgaaca cttcttcatc gttgaccgcc tgaagtctct
1501 gattaagtac aaaggctatc aggtggctcc cgctgaattg gaatccatct tgctccaaca
1561 ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc
1621 cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta
1681 cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga
1741 agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa
1801 ggccaagaag ggcggaaaga tcgccgtgta attctagagt cggggcggcc ggccgcttcg
1861 agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
1921 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
1981 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
2041 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc
2101 gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
2161 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
2221 cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
2281 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
2341 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
2401 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
2461 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
2521 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
2581 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
2641 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
2701 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
2761 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
2821 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
2881 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
2941 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
3001 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
3061 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
3121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
3181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
3241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
3301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
3361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
3421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
3481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
3541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
3601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
3661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
3721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
3781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
3841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
3901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
3961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
4021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
4081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
4141 tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
4201 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
4261 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
4321 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
4381 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
4441 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
4501 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
4561 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttgc
4621 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
4681 ttacgccagc ccaagctacc atgataagta agtaatatta aggtacggga ggtacttgga
4741 gcggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat
4801 cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat
4861 aggctgtccc cagtgcaagt gcaggtgcca gaacatttct ct
//
Caution:
Product is for research use only!
Search name
pNF-KB-TA-LUC,Plasmid pNF-KB-TA-LUC,pNF-KB-TA-LUC vector
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