pPARP11-Fluc plasmid

PVT109979

pPARP11-Fluc plasmid Information

Plasmid name: pPARP11(human)-Fluc
Alias:
Gene lenght: 2001
Host: Mammalian cells
Use(s): Signal report
Fragment type: Promoter
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc
Promoter: PARP11
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: RVP3
Reverse primer:
Copy number: High
Induction:

pPARP11-Fluc plasmid Description

pPARP11-Fluc is a plasmid suitable for promoter detection in mammalian cells.

pPARP11-Fluc plasmid Sequence

1 ggtaccccaa tgcaatagga agaaagaaac gcattatttg caggtgataa tgattgtctg 61 tgtacaaatt catagagatg agctgtcaag ctattagaac taacaagaca ttttttcata 121 gtgtggtagg atacaaggta aataaacaaa agtcaatagc tttcctaaat tacagcaata 181 agaaatatct cacttacaaa aaaactataa aatatctagg aacaaacttc ccaaggatta 241 tgcaaaccta tgtgattttt tttttttttt tacagatgct gatgcagatc tgaaaaaaaa 301 ttgttaatgc atctaaagat acagaaaaca agtatttctg aatggaaaga cccatgatat 361 tgaaaatttt gaaaaagaag agaattagga gaggttcctt tatgaaaagg caaatatatt 421 aaatatgtaa tgattataac tgtggcatat tttaggaata agcagataat taatagagga 481 gaatgttcag aaacagatat atacgtgtat gtatacctat gtatatgtat atgcaaattt 541 aggttgtaat taacagtggc acttcaaagt agtgaggaaa gaatattcac taaatgacat 601 gggggcaact ggtcaaccat ttggcaaaaa taatctctta gtaaactatt ttatttccag 661 gaattaattc caagaagtaa atggacaaat gtgaagaaat gcctgttgaa ggattttttt 721 gggatagtga aaaatagaaa accactcaaa cgtctattca gtagattatt tcaaatgtgt 781 gtgtctattt gtgtgtacgt agtatacgca taagttatgg aatgccatga gtctacttta 841 aaatatggcc gggtgcaaga tggctcacac ctgtaatccc agcactttgg gaggccaagg 901 caggtggatc acctggggtc aggagttcga gactggcctg gccaacatgg cgaaaacctg 961 tctctactaa aaatacaaaa attcgcggga cgtggtggcg cacatctgta atcccagcta 1021 ctcaggagcc tgaggcacgg gaatcgcttg aactcgggag gcggaggttg cagtgagcca 1081 aaatggcgcc actgcgctcc agcctgggcg acagagcgag gttctgccta aataaataaa 1141 taaaatgatg aaacagattt aaacttaatg gaatggaagg atagccacat aatctttaaa 1201 gtgaaaaaaa aatcaggttc taaatctata ggtattcttt gattacattt ttttcaatgt 1261 gtagaggcat agaaaagtcc agaaggatct ataatgagct gtttaatttt catttcctat 1321 ggggtagatc ctttgttgca tggaagatta tttcactctg ttagctctct ttacatcagt 1381 aggaatacat cctcatggtt ttaaacatct tgagggagct ttcctgacac tctactctag 1441 gtaaatatgc tcgcatagca ctctctattc accatattac ctataaatca tattgcactt 1501 tttaactttt ctaaactact ttttgctttc ttttctagat tataaaatct tgagggcagg 1561 gatagtatgt aaaggttccc ttgtgagcaa ataaccgtca cttattgaac tctgtgtgcc 1621 aaatactgtg ctaaatgctt tcaataatct cactcatagg gagatattac taccatctcc 1681 attttgagga tgaagatatt gaaagattat gaagtttctc ccaaatgcca ttgccaggcc 1741 tgtgtgactc ttggtcgtta cacggtgatc aataaaaatt tgtttcatag gaaagaaata 1801 cagccagccg tggctttaag atcactgggt gatacagttg gaccagaaac cctttaaact 1861 ccttcgtcag acacagactc gcaagccccg cccaggacga ccgcccgcgc gcgtatggat 1921 aagcgtgcag gggcggggcg agcttgtagg agtggggcgg agacagatct gactggccaa 1981 cgtcgggcgg aagtcgggag gttgggtaag cttggcattc cggtactgtt ggtaaagcca 2041 ccatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg 2101 gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa 2161 ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt 2221 ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg 2281 tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag 2341 ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca 2401 tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc 2461 aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg 2521 gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg 2581 attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg 2641 gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct 2701 cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg 2761 ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat 2821 ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt 2881 acaagattca aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc 2941 tgattgacaa atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct 3001 ctaaggaagt cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat 3061 atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg 3121 gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga 3181 aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg 3241 gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt 3301 ctggagacat agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt 3361 ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc 3421 aacaccccaa catcttcgac gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac 3481 ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg 3541 attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg 3601 acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca 3661 taaaggccaa gaagggcgga aagatcgccg tgtaattcta gagtcggggc ggccggccgc 3721 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt 3781 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa 3841 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg 3901 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg 3961 atccgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg 4021 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag 4081 gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 4141 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 4201 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 4261 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 4321 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 4381 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 4441 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt 4501 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 4561 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 4621 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 4681 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 4741 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 4801 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 4861 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 4921 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 4981 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 5041 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 5101 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 5161 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 5221 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 5281 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 5341 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 5401 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 5461 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 5521 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 5581 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 5641 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 5701 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 5761 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 5821 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 5881 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 5941 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 6001 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg 6061 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 6121 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 6181 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 6241 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 6301 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 6361 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 6421 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat 6481 ttcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc 6541 gctattacgc cagcccaagc taccatgata agtaagtaat attaaggtac gggaggtact 6601 tggagcggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt 6661 gaatcgatag tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca 6721 aaataggctg tccccagtgc aagtgcaggt gccagaacat ttctctatcg ata

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.