pQE-32
PVT0524 2ug
pQE-32 Informaiton
Replicon: Ori
Terminator: Lambda t0
Plasmid classification: Escherichia coli vector; pQE series expression plasmid
Plasmid size: 3462bp
Plasmid tagging: 6*His
Prokaryotic resistance: ampicillin Amp
Clonal strain: DH5 alpha
Culture conditions: 37 C, aerobic, LB
Expression host: M15
Culture conditions: 37 C, aerobic, LB
Induction mode: no need to induce
5'sequencing primers: pQE30-F: AGCGGATAACAATTTCACACAG
3'sequencing primers: pQE30-R: TTCTGAGGTCATTACTGGATC
pQE-32 Description
pQE-32 is a 3462bp vector designed to recombinant protein expression in Escherichia coli. Resistance is Amp.
pQE-32 Multiple cloning site
pQE-32 Sequence
LOCUS Exported 3462 bp ds-DNA circular SYN 08-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS PQE-32
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3462)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Thursday, September 8, 2016 from SnapGene Viewer 3.1.4
FEATURES Location/Qualifiers
source 1..3462
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 10..54
/note="T5 promoter"
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
protein_bind 30..46
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
protein_bind 62..78
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 97..108
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 127..144
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
terminator 209..303
/note="lambda t0 terminator"
/note="transcription terminator from phage lambda"
CDS 347..1006
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
terminator 1071..1157
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
misc_feature 1312..1452
/note="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(1638..2226)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2397..3257)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3258..3362)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
121 ggatctcacc atcaccatca ccatgggatc cgcatgcgag ctcggtaccc cgggtcgacc
181 tgcagccaag cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca
241 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
301 aatccaagct agcttggcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat
361 cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt
421 tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt
481 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg
541 cctgatgaat gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg
601 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct
661 ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc
721 gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt
781 ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa
841 cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat
901 gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct
961 taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag
1021 ttattggtgc ccttaaacgc ctggggtaat gactctctag cttgaggcat caaataaaac
1081 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
1141 tcctgagtag gacaaatccg ccctctagag ctgcctcgcg cgtttcggtg atgacggtga
1201 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
1261 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
1321 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
1381 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
1441 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
1501 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
1561 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
1621 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
1681 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
1741 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
1801 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
1861 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
1921 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
1981 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
2041 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
2101 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
2161 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
2221 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
2281 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
2341 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
2401 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
2461 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
2521 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
2581 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
2641 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
2701 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
2761 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
2821 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
2881 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
2941 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
3001 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
3061 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
3121 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
3181 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
3241 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
3301 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
3361 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
3421 acctataaaa ataggcgtat cacgaggccc tttcgtcttc ac
//
Caution:
Product is for research use only!