pRL1063a Plasmid

Alias:
Gene length:
Host: vibrio
Use(s): Protein expression
Fragment Type: ORF
Fragmented species:
Prokaryotic resistance: Kan,Str,Bleo
Screening Markers: Bleo
Fluorescent labeling:
Promoter:
replicon: p15A
Copy Number:
Competent cells: DH5a
Temperature: 37 Degrees
Back Bones:
Forward primer:
Reverse primer: Induction method:
Sequence:
ctgactcttatacacaagtagcgtcctgaacggaacctttcccgttttccaggcgggatcctctagaggatccccgggtaccgagctcattgatagcattgaatctagtaattgtaagctctattcgctaattggaagttcacatgatcttggggaaaatttggttgtattaagaaatttttatcaatcagtgacgaaggcagccttagcattagatgatggtttattggatttagagatagacattattgaacctcgatttgaggacgttacaagtattactgttaaggagcgtagattaaaaaatgaaattgaaaatgaattattagaattggcttaaataaacagaatcaccaaaaaggaatagagtatgaagtttggaaatatttgtttttcgtatcaaccaccaggtgaaactcataagcaagtaatggatcgctttgttcggcttggtatcgcctcagaagaggtagggtttgatacatattggaccttagaacatcattttacagagtttggtcttacgggaaatttatttgttgctgcggctaacctgttaggaagaactaaaacattaaatgttggcactatgggggttgttattccgacagcacacccagttcgacagttagaagacgttttattattagatcaaatgtcgaaaggtcgttttaattttggaaccgttcgagggctataccataaagattttcgagtatttggtgttgatatggaagagtctcgagcaattactcaaaatttctaccagatgataatggaaagcttacagacaggaaccattagctctgatagtgattacattcaatttcctaaggttgatgtatatcccaaagtgtactcaaaaaatgtaccaacctgtatgactgctgagtccgcaagtacgacagaatggctagcaatacaagggctaccaatggttcttagttggattattggtactaatgaaaaaaaagcacagatggaactctataatgaaattgcgacagaatatggtcatgatatatctaaaatagatcattgtatgacttatatttgttctgttgatgatgatgcacaaaaggcgcaagatgtttgtcgggagtttctgaaaaattggtatgactcatatgtaaatgcgaccaatatctttaatgatagcaatcaaactcgtggttatgattatcataaaggtcaatggcgtgattttgttttacaaggacatacaaacaccaatcgacgtgttgattatagcaatggtattaaccctgtaggcactcctgagcagtgtattgaaatcattcaacgtgatattgatgcaacgggtattacaaacattacatgcggatttgaagctaatggaactgaagatgaaataattgcttccatgcgacgctttatgacacaagtcgctcctttcttaaaagaacctaaataaattacttatttgatactagagataataaggaacaagttatgaaatttggattattttttctaaactttcagaaagatggaataacatctgaagaaacgttggataatatggtaaagactgtcacgttaattgattcaactaaatatcattttaatactgcctttgttaatgaacatcacttttcaaaaaatggtattgttggagcacctattaccgcagctggttttttattagggttaacaaataaattacatattggttcattaaatcaagtaattaccacccatcaccctgtacgtgtagcagaagaagccagtttattagatcaaatgtcagagggacgcttcattcttggttttagtgactgcgaaagtgatttcgaaatggaattttttagacgtcatatctcatcaaggcaacaacaatttgaagcatgctatgaaataattaatgacgcattaactacaggttattgtcatccccaaaacgacttttatgattttccaaaggtttcaattaatccacactgttacagtgagaatggacctaagcaatatgtatccgctacatcaaaagaagtcgtcatgtgggcagcgaaaaaggcactgcctttaacatttaagtgggaggataatttagaaaccaaagaacgctatgcaattctatataataaaacagcacaacaatatggtattgatatttcggatgttgatcatcaattaactgtaattgcgaacttaaatgctgatagaagtacggctcaagaagaagtgagagaatacttaaaagactatatcactgaaacttaccctcaaatggacagagatgaaaaaattaactgcattattgaagagaatgcagttgggtctcatgatgactattatgaatcgacaaaattagcagtggaaaaaacagggtctaaaaatattttattatcctttgaatcaatgtccgatattaaagatgtaaaagatattattgatatgttgaaccaaaaaatcgaaatgaatttaccataataaaattaaaggcaatttctatattagattgcctttttggggatcctctagaaatattttatctgattaataagatgatcttcttgagatcgttttggtctgcgcgtaatctcttgctctgaaaacgaaaaaaccgccttgcagggcggtttttcgaaggttctctgagctaccaactctttgaaccgaggtaactggcttggaggagcgcagtcaccaaaacttgtcctttcagtttagccttaaccggcgcatgacttcaagactaactcctctaaatcaattaccagtggctgctgccagtggtgcttttgcatgtctttccgggttggactcaagacgatagttaccggataaggcgcagcggtcggactgaacggggggttcgtgcatacagtccagcttggagcgaactgcctacccggaactgagtgtcaggcgtggaatgagacaaacgcggccataacagcggaatgacaccggtaaaccgaaaggcaggaacaggagagcgcacgagggagccgccaggggaaacgcctggtatctttatagtcctgtcgggtttcgccaccactgatttgagcgtcagatttcgtgatgcttgtcaggggggcggagcctatggaaaaacggctttgccgcggccctctcacttccctgttaagtatcttcctggcatcttccaggaaatctccgccccgttcgtaagccatttccgctcgccgcagtcgaacgaccgagcgtagcgagtcagtgagcgaggaagcggaatatatcctgtatcacatattctgctgacgcaccggtgcagccttttttctcctgccacatgaagcacttcactgacaccctcatcagtgccaacatagtaagccagtatacactccgctagaggatctcaatgaatattggttgacacgggcgtataagacatgttatactgttgaataacaaggacggatctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgcgcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgacgcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccgggctcgatcccctcgcgagttggttcagctgctgcctgaggctggacgacctcgcggagttctaccggcagtgcaaatccgtcggcatccaggaaaccagcagcggctatccgcgcatccatgcccccgaactgcaggagtggggaggcacgatggccgctttggtcgacccggacgggacgctcctgcgcctgatacagaacgaattgcttgcaggcatctcatgagtgtgtcttcccgttttccgcctgaggtcactgcgtggatggagcgctggcgcctgctgcgcgacggcgagctgctcaccacccactcgagctggatacttcccgtccgccagggggacatgccggcgatgctgaaggtcgcgcgcattcccgatgaagaggccggttaccgcctgttgacctggtgggacgggcagggcgccgcccgagtcttcgcctcggcggcgggcgctctgctcatggagcgcgcgtccggggccggggaccttgcacagatagcgtggtccggccaggacgacgaggcttgcaggatcctctgcgacaccgccgctcgtctgcacgcgccgcggtccggaccgccgcccgatctccatccgctacaggaatggttccagccgcttttccggttggccgctgagcacgcggcacttgcgcccgccgccagcgtagcgcgccaacttctggcggcgccgcgcgaggtgtgcccgctccacggcgacctgcaccacgagaacgtgctcgacttcggcgaccgcggctggctggccagcgacccgcacggactgctcggcgagcgcaccttcgactatgccaacatcttcacgaatcccgatctcagcgaccccggtcgcccgcttgcgatcctgccgggcaggctggaggctcgactcagcattgtggtcgcgacgaccgggtttgagcccgaacggcttcttcgctggatcattgcatggacgggcttgtcggcagcctggttcatcggcgacggcgacggcgagggcgctgcgattgatctggccgtaaacgccatggcacgccggttgcttgactagcgcggtcaccgatctcacctggtcgtcgagctaggtcaggccgtgtcgggcgtgatccgctggaagtcgttgcgggccacacccgccgcctcgaagccctgcaccaggccggcatcgtggtgtgcgtggccgagggactatggaaggtgccggacgatctgcccgagcagggccgccgctatgacgcccgggagtcagcgcagaatggtgaaccagagcaagggaatatgttacgcgtgacgcagtagcggtaaacggcagacaaaaaaatgtcgcacaatgtgcgccatttttcacttcacaggtggaattgtacccttggtggcgtgacggtggagctgaaatcgcacctgcccatcgagcggcaggcccgcgtgatcggtgccacctggcttgaccagcagttgatcgacggtggctcgggcttgggcgacctgggctttagcagtgaggccaagtaggcgatacagcagcgcgcggacttcctggccgaacagggactggccgagcggcgcgggcagcgcgtgatcctcaccggaatctgctcggcagcagcgggctcgggaactggcgcaggccgcgaaggacattgccgccgataccggcctggagcatcgccccgtggccgacggccagcgcgttgccggcgtctaccggcgccccgtcatgctcgccagcgggcgaaatgggatgcttgatgacgccaaggggtccagcctcgtgcggtggaagcccatcgaacagcggcttggggagcagctcgccgcgacggtgcgcggtggcggcgtgtcttgggagattggacgacagcgtgggccggcccctgtctcttgatcagatcttgatcccctgcgccatcagatccttggcggcaagaaagccatccagtttactttgcagggcttcccaaccttcccagagggcgccccagctggcaattccggttcgcttgctgtccataaaaccgcccagtctagctatcgccatgtaagcccactgcaagctacctgctttctctttgcgcttgcgttttcccttgtccagatagcccagtagctgacattcatccggggtcagcaccgtttctgcggactggctttctacgtgttccgcttcctttagcagcccttgcgccctgagtgcttgcggcagcgtgaagctttctctgagctgtaacagcctgaccgcaacaaacgagaggatcgagaccatccgctccagattatccggctcctccatgcgttgcctctcggctcctgctccggttttccatgccttatggaactcctcgatccgccagcgatgggtataaatgtcgatgacgcgcaaggcttgggctagcgactcgaccggttcgccggtcagcaacaaccatttcaacggggtctcacccttgggcgggttaatctcctcggccagcaccgcgttgagcgtgatattcccctgttttagcgtgatgcgcccactgcgcaggctcaagctcgccttgcgggctggtcgatttttacgtttaccgcgtttatccaccacgcccttttgcggaatgctgatctgatagccacccaactccggttggttcttcagatggtcgatcagatacaacccagactctacgtccttgcgtgggtgcttggagcgcaccacgaagcgctcgttatgcgccagcctgtcctgcagataagcatgaatatcggcttcgcggtcacagaccgcaatcacgttgctcatcatgctgcccatgcgtaaccggctagttgcggccgctgccagccatttgccactctccttttcatccgcatcggcagggtcatccgggcgcatccaccactcctgatgcagtaatcctacggtgcggaatgtggtggcctcgagcaagagaacggagtgaacccaccatccgcgggatttatcctgaatagagcccagcttgccaagctcttcggcgacctggtggcgataactcaaagaggtggtgtcctcaatggccagcagttcgggaaactcctgagccaacttgactgtttgcatggcgccagcctttctgatcgcctcggcagaaacgttgggattgcggtaaaatcggtaagcgccttcctgcatggcttcactaccctctgatgtgatggggattgatttaccagaatattttgccaattgggcggcgacgttaaccaagcgggcagtacggcgtggatcacccagcgccgccgaagagaacacagatttagcccagtcggccgcacgatgaagagcagaagttatcatgaacgttaccatgttaggaggtcacatggaatatcagattctggaaaacgggaaaggttccgttcaggacgctacttgtgtataagagtcaggtgagttatgttgttgctttactttttagcttgcttgctggaactacattaggtatttggggtattgctattgttacaggaattcatcgatatctagatctcgagctcgcgaaagcttgcatgcctgcaggtccccgggatcgtcttgccttgctcgtcggtgatgtacttcaccagctccgcgaagtcgctcttcttgatggagcgcatggggacgtgcttggcaatcacgcgcaccccccggccgttttagcggctaaaaaagtcatggctctgccctcgggcggaccacgcccatcatgaccttgccaagctcgtcctgcttctcttcgatcttcgccagcagggcgaggatcgtggcatcaccgaaccgcgccgtgcgcgggtcgtcggtgagccagagtttcagcaggccgcccaggcggcccaggtcgccattgatgcgggccagctcgcggacgtgctcatagtccacgacgcccgtgattttgtagccctggccgacggccagcaggtaggccgacaggctcatgccggccgccgccgccttttcctcaatcgctcttcgttcgtctggaaggcagtacaccttgataggtgggctgcccttcctggttggcttggtttcatcagccatccgcttgccctcatctgttacgccggcggtagccggccagcctcgcagagcaggattcccgttgagcaccgccaggtgcgaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgcccggctgacgccgttggatacaccaaggaaagtctacacgaaccctttggcaaaatcctgtatatcgtgcgaaaaaggatggatataccgaaaaaatcgctataatgaccccgaagcagggttatgcagcggaagatcgtcgacctgcaggcatgcaagctttcgcgagctcgagatctagaatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagc
//

Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3. Shipping temperature is 2-8 degrees centigrade.
4.;