pSIN-EF1a-DsRed2 plasmid

PVT93719

pSIN-EF1a-DsRed2 plasmid Information

Plasmid name: pSIN-EF1a-DsRed2
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: EF1a-R-F
Reverse primer:
Copy number: High
Induction:

pSIN-EF1a-DsRed2 plasmid Description

pSIN-EF1a-DsRed2 is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pSIN-EF1a-DsRed2 plasmid Sequence

1 CGGACCGCCA CTGCCAATTA CCTGTGGTTT CATTTACTCT AAACCTGTGA TTCCTCTGAA 61 TTATTTTCAT TTTAAAGAAA TTGTATTTGT TAAATATGTA CTACAAACTT AGTAGTTGGA 121 AGGGCTAATT CACTCCCAAA GAAGACAAGA TATCCTTGAT CTGTGGATCT ACCACACACA 181 AGGCTACTTC CCTGATTAGC AGAACTACAC ACCAGGGCCA GGGGTCAGAT ATCCACTGAC 241 CTTTGGATGG TGCTACAAGC TAGTACCAGT TGAGCCAGAT AAGGTAGAAG AGGCCAATAA 301 AGGAGAGAAC ACCAGCTTGT TACACCCTGT GAGCCTGCAT GGGATGGATG ACCCGGAGAG 361 AGAAGTGTTA GAGTGGAGGT TTGACAGCCG CCTAGCATTT CATCACGTGG CCCGAGAGCT 421 GCATCCGGAG TACTTCAAGA ACTGCTGATA TCGAGCTTGC TACAAGGGAC TTTCCGCTGG 481 GGACTTTCCA GGGAGGCGTG GCCTGGGCGG GACTGGGGAG TGGCGAGCCC TCAGATCCTG 541 CATATAAGCA GCTGCTTTTT GCCTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT 601 GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG 661 TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC 721 CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GTGGCGCCCG AACAGGGACT TGAAAGCGAA 781 AGGGAAACCA GAGGAGCTCT CTCGACGCAG GACTCGGCTT GCTGAAGCGC GCACGGCAAG 841 AGGCGAGGGG CGGCGACTGG TGAGTACGCC AAAAATTTTG ACTAGCGGAG GCTAGAAGGA 901 GAGAGATGGG TGCGAGAGCG TCAGTATTAA GCGGGGGAGA ATTAGATCGC GATGGGAAAA 961 AATTCGGTTA AGGCCAGGGG GAAAGAAAAA ATATAAATTA AAACATATAG TATGGGCAAG 1021 CAGGGAGCTA GAACGATTCG CAGTTAATCC TGGCCTGTTA GAAACATCAG AAGGCTGTAG 1081 ACAAATACTG GGACAGCTAC AACCATCCCT TCAGACAGGA TCAGAAGAAC TTAGATCATT 1141 ATATAATACA GTAGCAACCC TCTATTGTGT GCATCAAAGG ATAGAGATAA AAGACACCAA 1201 GGAAGCTTTA GACAAGATAG AGGAAGAGCA AAACAAAAGT AAGACCACCG CACAGCAAGC 1261 GGCCGCTGAT CTTCAGACCT GGAGGAGGAG ATATGAGGGA CAATTGGAGA AGTGAATTAT 1321 ATAAATATAA AGTAGTAAAA ATTGAACCAT TAGGAGTAGC ACCCACCAAG GCAAAGAGAA 1381 GAGTGGTGCA GAGAGAAAAA AGAGCAGTGG GAATAGGAGC TTTGTTCCTT GGGTTCTTGG 1441 GAGCAGCAGG AAGCACTATG GGCGCAGCGT CAATGACGCT GACGGTACAG GCCAGACAAT 1501 TATTGTCTGG TATAGTGCAG CAGCAGAACA ATTTGCTGAG GGCTATTGAG GCGCAACAGC 1561 ATCTGTTGCA ACTCACAGTC TGGGGCATCA AGCAGCTCCA GGCAAGAATC CTGGCTGTGG 1621 AAAGATACCT AAAGGATCAA CAGCTCCTGG GGATTTGGGG TTGCTCTGGA AAACTCATTT 1681 GCACCACTGC TGTGCCTTGG AATGCTAGTT GGAGTAATAA ATCTCTGGAA CAGATTTGGA 1741 ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT 1801 CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG 1861 ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT 1921 TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA 1981 TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC 2041 CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA 2101 GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGCCA CAAATGGCAG TATTCATCCA 2161 CAATTTTAAA AGAAAGGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 2221 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT 2281 TCGGGTTTAT TACAGGGACA GCAGAGATCC ACTTTGGATC GATAAGCTTT GCAAAGATGG 2341 ATAAAGTTTT AAACAGAGAG GAATCTTTGC AGCTAATGGA CCTTCTAGGT CTTGAAAGGA 2401 GTGGGAATTG GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA CATCGCCCAC AGTCCCCGAG 2461 AAGTTGGGGG GAGGGGTCGG CAATTGAACC GGTGCCTAGA GAAGGTGGCG CGGGGTAAAC 2521 TGGGAAAGTG ATGTCGTGTA CTGGCTCCGC CTTTTTCCCG AGGGTGGGGG AGAACCGTAT 2581 ATAAGTGCAG TAGTCGCCGT GAACGTTCTT TTTCGCAACG GGTTTGCCGC CAGAACACAG 2641 GTAAGTGCCG TGTGTGGTTC CCGCGGGCCT GGCCTCTTTA CGGGTTATGG CCCTTGCGTG 2701 CCTTGAATTA CTTCCACCTG GCTGCAGTAC GTGATTCTTG ATCCCGAGCT TCGGGTTGGA 2761 AGTGGGTGGG AGAGTTCGAG GCCTTGCGCT TAAGGAGCCC CTTCGCCTCG TGCTTGAGTT 2821 GAGGCCTGGC CTGGGCGCTG GGGCCGCCGC GTGCGAATCT GGTGGCACCT TCGCGCCTGT 2881 CTCGCTGCTT TCGATAAGTC TCTAGCCATT TAAAATTTTT GATGACCTGC TGCGACGCTT 2941 TTTTTCTGGC AAGATAGTCT TGTAAATGCG GGCCAAGATC TGCACACTGG TATTTCGGTT 3001 TTTGGGGCCG CGGGCGGCGA CGGGGCCCGT GCGTCCCAGC GCACATGTTC GGCGAGGCGG 3061 GGCCTGCGAG CGCGGCCACC GAGAATCGGA CGGGGGTAGT CTCAAGCTGG CCGGCCTGCT 3121 CTGGTGCCTG GCCTCGCGCC GCCGTGTATC GCCCCGCCCT GGGCGGCAAG GCTGGCCCGG 3181 TCGGCACCAG TTGCGTGAGC GGAAAGATGG CCGCTTCCCG GCCCTGCTGC AGGGAGCTCA 3241 AAATGGAGGA CGCGGCGCTC GGGAGAGCGG GCGGGTGAGT CACCCACACA AAGGAAAAGG 3301 GCCTTTCCGT CCTCAGCCGT CGCTTCATGT GACTCCACGG AGTACCGGGC GCCGTCCAGG 3361 CACCTCGATT AGTTCTCGAG CTTTTGGAGT ACGTCGTCTT TAGGTTGGGG GGAGGGGTTT 3421 TATGCGATGG AGTTTCCCCA CACTGAGTGG GTGGAGACTG AAGTTAGGCC AGCTTGGCAC 3481 TTGATGTAAT TCTCCTTGGA ATTTGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG 3541 CCTCAGACAG TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAGG AATTGGGATC 3601 Cgccaccatg gcctcctccg agaacgtcat caccgagttc atgcgcttca aggtgcgcat 3661 ggagggcacc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta 3721 cgagggccac aacaccgtga agctgaaggt gaccaagggc ggccccctgc ccttcgcctg 3781 ggacatcctg tccccccagt tccagtacgg ctccaaggtg tacgtgaagc accccgccga 3841 catccccgac tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa 3901 cttcgaggac ggcggcgtgg cgaccgtgac ccaggactcc tccctgcagg acggctgctt 3961 catctacaag gtgaagttca tcggcgtgaa cttcccctcc gacggccccg tgatgcagaa 4021 gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa 4081 gggcgagacc cacaaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa 4141 gtccatctac atggccaaga agcccgtgca gctgcccggc tactactacg tggacgccaa 4201 gctggacatc acctcccaca acgaggacta caccatcgtg gagcagtacg agcgcaccga 4261 gggccgccac cacctgttcc tgtagGAATT CTTCGAACAT ATGAAGCTTG CTAGCACTAG 4321 TGATCCGCAT GCATCTAGGG CGGCCAATTC CGCCCCTCTC CCCCCCCCCC CTCTCCCTCC 4381 CCCCCCCCTA ACGTTACTGG CCGAAGCCGC TTGGAATAAG GCCGGTGTGC GTTTGTCTAT 4441 ATGTTATTTT CCACCATATT GCCGTCTTTT GGCAATGTGA GGGCCCGGAA ACCTGGCCCT 4501 GTCTTCTTGA CGAGCATTCC TAGGGGTCTT TCCCCTCTCG CCAAAGGAAT GCAAGGTCTG 4561 TTGAATGTCG TGAAGGAAGC AGTTCCTCTG GAAGCTTCTT GAAGACAAAC AACGTCTGTA 4621 GCGACCCTTT GCAGGCAGCG GAACCCCCCA CCTGGCGACA GGTGCCTCTG CGGCCAAAAG 4681 CCACGTGTAT AAGATACACC TGCAAAGGCG GCACAACCCC AGTGCCACGT TGTGAGTTGG 4741 ATAGTTGTGG AAAGAGTCAA ATGGCTCTCC TCAAGCGTAT TCAACAAGGG GCTGAAGGAT 4801 GCCCAGAAGG TACCCCATTG TATGGGATCT GATCTGGGGC CTCGGTGCAC ATGCTTTACA 4861 TGTGTTTAGT CGAGGTTAAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA CGTGGTTTTC 4921 CTTTGAAAAA CACGATGATA AGCTTGCCAC AACCCACAAG GAGACGACCT TCCATGACCG 4981 AGTACAAGCC CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCCGGGCC GTACGCACCC 5041 TCGCCGCCGC GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGACCCG GACCGCCACA 5101 TCGAGCGGGT CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA 5161 AGGTGTGGGT CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG 5221 AAGCGGGGGC GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC 5281 TGGCCGCGCA GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT 5341 GGTTCCTGGC CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG 5401 TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT 5461 CCGCGCCCCG CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG 5521 AGGTGCCCGA AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGATAATAGG 5581 CGGCCGCTCG AGACCTAGAA AAACATGGAG CAATCACAAG TAGCAATACA GCAGCTACCA 5641 ATGCTGATTG TGCCTGGCTA GAAGCACAAG AGGAGGAGGA GGTGGGTTTT CCAGTCACAC 5701 CTCAGGTACC TTTAAGACCA ATGACTTACA AGGCAGCTGT AGATCTTAGC CACTTTTTAA 5761 AAGAAAAGGG GGGACTGGAA GGGCTAATTC ACTCCCAACG AAGACAAGAT CTGCTTTTTG 5821 CTTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG 5881 GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC 5941 GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA 6001 TCTCTAGCAG TAGTAGTTCA TGTCATCTTA TTATTCAGTA TTTATAACTT GCAAAGAAAT 6061 GAATATCAGA GAGTGAGAGG CCTTGACATT ATAATAGATT TAGCAGGAAT TGAACTAGGA 6121 GTGGAGCACA CAGGCAAAGT TCTAGAGCTC GCTGATCAGC CTCGACTGTG CCTTCTAGTT 6181 GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC 6241 CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT 6301 CTATTCTGGG GGGTGGGGGT GGGGCAGGAC AGCAAGGGGG GAGGATTGGG AAGACAATAG 6361 CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA CCAGCTGGGG 6421 GCGCGCCCCT CGAGGCCGCC ATGGTCATAG CTGTTTGACG TCAGGTGGCA CTTTTCGGGG 6481 AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT 6541 CACGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT 6601 TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC 6661 TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG 6721 TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG 6781 TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA 6841 CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA 6901 CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC 6961 TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC 7021 GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG 7081 GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC 7141 AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA 7201 ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT 7261 TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT 7321 CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG 7381 GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT 7441 TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT 7501 TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT 7561 CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC 7621 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT 7681 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG 7741 CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA 7801 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC 7861 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA 7921 TAAGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG 7981 ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA 8041 GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG 8101 GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA 8161 CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC 8221 AACGCGGCCT TTTTACGGTT CTGGCCTTTT GCA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???