pSIN-EF2-BRD2-3×FLAG-Puro plasmid

PVT105571

pSIN-EF2-BRD2-3×FLAG-Puro plasmid Information

Plasmid name: pSIN-EF2-BRD2(human)-3×FLAG-Puro
Alias: NM_001113182.3;BRD2-IT1; D6S113E; FSH; FSHRG1; FSRG1; NAT;O27.1.1; RING3; RNF3
Gene lenght: 2403
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: EF-1α-F1
Reverse primer:
Copy number: High
Induction:

pSIN-EF2-BRD2-3×FLAG-Puro plasmid Description

pSIN-EF2-BRD2-3×FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pSIN-EF2-BRD2-3×FLAG-Puro plasmid Sequence

1 CGGACCGCCA CTGCCAATTA CCTGTGGTTT CATTTACTCT AAACCTGTGA TTCCTCTGAA 61 TTATTTTCAT TTTAAAGAAA TTGTATTTGT TAAATATGTA CTACAAACTT AGTAGTTGGA 121 AGGGCTAATT CACTCCCAAA GAAGACAAGA TATCCTTGAT CTGTGGATCT ACCACACACA 181 AGGCTACTTC CCTGATTAGC AGAACTACAC ACCAGGGCCA GGGGTCAGAT ATCCACTGAC 241 CTTTGGATGG TGCTACAAGC TAGTACCAGT TGAGCCAGAT AAGGTAGAAG AGGCCAATAA 301 AGGAGAGAAC ACCAGCTTGT TACACCCTGT GAGCCTGCAT GGGATGGATG ACCCGGAGAG 361 AGAAGTGTTA GAGTGGAGGT TTGACAGCCG CCTAGCATTT CATCACGTGG CCCGAGAGCT 421 GCATCCGGAG TACTTCAAGA ACTGCTGATA TCGAGCTTGC TACAAGGGAC TTTCCGCTGG 481 GGACTTTCCA GGGAGGCGTG GCCTGGGCGG GACTGGGGAG TGGCGAGCCC TCAGATCCTG 541 CATATAAGCA GCTGCTTTTT GCCTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT 601 GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG 661 TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC 721 CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GTGGCGCCCG AACAGGGACT TGAAAGCGAA 781 AGGGAAACCA GAGGAGCTCT CTCGACGCAG GACTCGGCTT GCTGAAGCGC GCACGGCAAG 841 AGGCGAGGGG CGGCGACTGG TGAGTACGCC AAAAATTTTG ACTAGCGGAG GCTAGAAGGA 901 GAGAGATGGG TGCGAGAGCG TCAGTATTAA GCGGGGGAGA ATTAGATCGC GATGGGAAAA 961 AATTCGGTTA AGGCCAGGGG GAAAGAAAAA ATATAAATTA AAACATATAG TATGGGCAAG 1021 CAGGGAGCTA GAACGATTCG CAGTTAATCC TGGCCTGTTA GAAACATCAG AAGGCTGTAG 1081 ACAAATACTG GGACAGCTAC AACCATCCCT TCAGACAGGA TCAGAAGAAC TTAGATCATT 1141 ATATAATACA GTAGCAACCC TCTATTGTGT GCATCAAAGG ATAGAGATAA AAGACACCAA 1201 GGAAGCTTTA GACAAGATAG AGGAAGAGCA AAACAAAAGT AAGACCACCG CACAGCAAGC 1261 GGCCGCTGAT CTTCAGACCT GGAGGAGGAG ATATGAGGGA CAATTGGAGA AGTGAATTAT 1321 ATAAATATAA AGTAGTAAAA ATTGAACCAT TAGGAGTAGC ACCCACCAAG GCAAAGAGAA 1381 GAGTGGTGCA GAGAGAAAAA AGAGCAGTGG GAATAGGAGC TTTGTTCCTT GGGTTCTTGG 1441 GAGCAGCAGG AAGCACTATG GGCGCAGCGT CAATGACGCT GACGGTACAG GCCAGACAAT 1501 TATTGTCTGG TATAGTGCAG CAGCAGAACA ATTTGCTGAG GGCTATTGAG GCGCAACAGC 1561 ATCTGTTGCA ACTCACAGTC TGGGGCATCA AGCAGCTCCA GGCAAGAATC CTGGCTGTGG 1621 AAAGATACCT AAAGGATCAA CAGCTCCTGG GGATTTGGGG TTGCTCTGGA AAACTCATTT 1681 GCACCACTGC TGTGCCTTGG AATGCTAGTT GGAGTAATAA ATCTCTGGAA CAGATTTGGA 1741 ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT 1801 CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG 1861 ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT 1921 TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA 1981 TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC 2041 CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA 2101 GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGCCA CAAATGGCAG TATTCATCCA 2161 CAATTTTAAA AGAAAGGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 2221 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT 2281 TCGGGTTTAT TACAGGGACA GCAGAGATCC ACTTTGGATC GATAAGCTTT GCAAAGATGG 2341 ATAAAGTTTT AAACAGAGAG GAATCTTTGC AGCTAATGGA CCTTCTAGGT CTTGAAAGGA 2401 GTGGGAATTG GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA CATCGCCCAC AGTCCCCGAG 2461 AAGTTGGGGG GAGGGGTCGG CAATTGAACC GGTGCCTAGA GAAGGTGGCG CGGGGTAAAC 2521 TGGGAAAGTG ATGTCGTGTA CTGGCTCCGC CTTTTTCCCG AGGGTGGGGG AGAACCGTAT 2581 ATAAGTGCAG TAGTCGCCGT GAACGTTCTT TTTCGCAACG GGTTTGCCGC CAGAACACAG 2641 GTAAGTGCCG TGTGTGGTTC CCGCGGGCCT GGCCTCTTTA CGGGTTATGG CCCTTGCGTG 2701 CCTTGAATTA CTTCCACCTG GCTGCAGTAC GTGATTCTTG ATCCCGAGCT TCGGGTTGGA 2761 AGTGGGTGGG AGAGTTCGAG GCCTTGCGCT TAAGGAGCCC CTTCGCCTCG TGCTTGAGTT 2821 GAGGCCTGGC CTGGGCGCTG GGGCCGCCGC GTGCGAATCT GGTGGCACCT TCGCGCCTGT 2881 CTCGCTGCTT TCGATAAGTC TCTAGCCATT TAAAATTTTT GATGACCTGC TGCGACGCTT 2941 TTTTTCTGGC AAGATAGTCT TGTAAATGCG GGCCAAGATC TGCACACTGG TATTTCGGTT 3001 TTTGGGGCCG CGGGCGGCGA CGGGGCCCGT GCGTCCCAGC GCACATGTTC GGCGAGGCGG 3061 GGCCTGCGAG CGCGGCCACC GAGAATCGGA CGGGGGTAGT CTCAAGCTGG CCGGCCTGCT 3121 CTGGTGCCTG GCCTCGCGCC GCCGTGTATC GCCCCGCCCT GGGCGGCAAG GCTGGCCCGG 3181 TCGGCACCAG TTGCGTGAGC GGAAAGATGG CCGCTTCCCG GCCCTGCTGC AGGGAGCTCA 3241 AAATGGAGGA CGCGGCGCTC GGGAGAGCGG GCGGGTGAGT CACCCACACA AAGGAAAAGG 3301 GCCTTTCCGT CCTCAGCCGT CGCTTCATGT GACTCCACGG AGTACCGGGC GCCGTCCAGG 3361 CACCTCGATT AGTTCTCGAG CTTTTGGAGT ACGTCGTCTT TAGGTTGGGG GGAGGGGTTT 3421 TATGCGATGG AGTTTCCCCA CACTGAGTGG GTGGAGACTG AAGTTAGGCC AGCTTGGCAC 3481 TTGATGTAAT TCTCCTTGGA ATTTGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG 3541 CCTCAGACAG TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAGG AATTGGGATC 3601 Cgccaccatg ctgcaaaacg tgactcccca caataagctc cctggggaag ggaatgcagg 3661 gttgctgggg ctgggcccag aagcagcagc accagggaag aggattcgaa aaccctctct 3721 cttgtatgag ggctttgaga gccccacaat ggcttcggtg cctgctttgc aacttacccc 3781 tgccaaccca ccacccccgg aggtgtccaa tcccaaaaag ccaggacgag ttaccaacca 3841 gctgcaatac ctacacaagg tagtgatgaa ggctctgtgg aaacatcagt tcgcatggcc 3901 attccggcag cctgtggatg ctgtcaaact gggtctaccg gattatcaca aaattataaa 3961 acagcctatg gacatgggta ctattaagag gagacttgaa aacaattatt attgggctgc 4021 ttcagagtgt atgcaagatt ttaataccat gttcaccaac tgttacattt acaacaagcc 4081 cactgatgat attgtcctaa tggcacaaac gctggaaaag atattcctac agaaggttgc 4141 atcaatgcca caagaagaac aagagctggt agtgaccatc cctaagaaca gccacaagaa 4201 gggggccaag ttggcagcgc tccagggcag tgttaccagt gcccatcagg tgcctgccgt 4261 ctcttctgtg tcacacacag ccctgtatac tcctccacct gagataccta ccactgtcct 4321 caacattccc cacccatcag tcatttcctc tccacttctc aagtccttgc actctgctgg 4381 acccccgctc cttgctgtta ctgcagctcc tccagcccag ccccttgcca agaaaaaagg 4441 cgtaaagcgg aaagcagata ctaccacccc tacacctaca gccatcttgg ctcctggttc 4501 tccagctagc cctcctggga gtcttgagcc taaggcagca cggcttcccc ctatgcgtag 4561 agagagtggt cgccccatca agcccccacg caaagacttg cctgactctc agcaacaaca 4621 ccagagctct aagaaaggaa agctttcaga acagttaaaa cattgcaatg gcattttgaa 4681 ggagttactc tctaagaagc atgctgccta tgcttggcct ttctataaac cagtggatgc 4741 ttctgcactt ggcctgcatg actaccatga catcattaag caccccatgg acctcagcac 4801 tgtcaagcgg aagatggaga accgtgatta ccgggatgca caggagtttg ctgctgatgt 4861 acggcttatg ttctccaact gctataagta caatccccca gatcacgatg ttgtggcaat 4921 ggcacgaaag ctacaggatg tatttgagtt ccgttatgcc aagatgccag atgaaccact 4981 agaaccaggg cctttaccag tctctactgc catgccccct ggcttggcca aatcgtcttc 5041 agagtcctcc agtgaggaaa gtagcagtga gagctcctct gaggaagagg aggaggaaga 5101 tgaggaggac gaggaggaag aagagagtga aagctcagac tcagaggaag aaagggctca 5161 tcgcttagca gaactacagg aacagcttcg ggcagtacat gaacaactgg ctgctctgtc 5221 ccagggtcca atatccaagc ccaagaggaa aagagagaaa aaagagaaaa agaagaaacg 5281 gaaggcagag aagcatcgag gccgagctgg ggccgatgaa gatgacaagg ggcctagggc 5341 accccgccca cctcaaccta agaagtccaa gaaagcaagt ggcagtgggg gtggcagtgc 5401 tgctttaggc ccttctggct ttggaccttc tggaggaagt ggcaccaagc tccccaaaaa 5461 ggccacaaag acagccccac ctgccctgcc tacaggttat gattcagagg aggaggaaga 5521 gagcaggccc atgagttacg atgagaagcg gcagctgagc ctggacatca acaaattacc 5581 tggggagaag ctgggccgag ttgtgcatat aatccaagcc agggagccct ctttacgtga 5641 ttcaaaccca gaagagattg agattgattt tgaaacactc aagccatcca cacttagaga 5701 gcttgagcgc tatgtccttt cctgcctacg taagaaaccc cggaagccct acaccattaa 5761 gaagcctgtg ggaaagacaa aggaggaact ggctttggag aaaaagcggg aattagaaaa 5821 gcggttacaa gatgtcagcg gacagctcaa ttctactaaa aagcccccca agaaagcgaa 5881 tgagaaaaca gagtcatcct ctgcacagca agtagcagtg tcacgcctta gcgcttccag 5941 ctccagctca gattccagct cctcctcttc ctcgtcgtcg tcttcagaca ccagtgattc 6001 agactcaggc GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA 6061 GGATGACGAT GACAAGTAGG AATTCTTCGA ACATATGAAG CTTGCTAGCA CTAGTGATCC 6121 GCATGCATCT AGGGCGGCCA ATTCCGCCCC TCTCCCCCCC CCCCCTCTCC CTCCCCCCCC 6181 CCTAACGTTA CTGGCCGAAG CCGCTTGGAA TAAGGCCGGT GTGCGTTTGT CTATATGTTA 6241 TTTTCCACCA TATTGCCGTC TTTTGGCAAT GTGAGGGCCC GGAAACCTGG CCCTGTCTTC 6301 TTGACGAGCA TTCCTAGGGG TCTTTCCCCT CTCGCCAAAG GAATGCAAGG TCTGTTGAAT 6361 GTCGTGAAGG AAGCAGTTCC TCTGGAAGCT TCTTGAAGAC AAACAACGTC TGTAGCGACC 6421 CTTTGCAGGC AGCGGAACCC CCCACCTGGC GACAGGTGCC TCTGCGGCCA AAAGCCACGT 6481 GTATAAGATA CACCTGCAAA GGCGGCACAA CCCCAGTGCC ACGTTGTGAG TTGGATAGTT 6541 GTGGAAAGAG TCAAATGGCT CTCCTCAAGC GTATTCAACA AGGGGCTGAA GGATGCCCAG 6601 AAGGTACCCC ATTGTATGGG ATCTGATCTG GGGCCTCGGT GCACATGCTT TACATGTGTT 6661 TAGTCGAGGT TAAAAAAACG TCTAGGCCCC CCGAACCACG GGGACGTGGT TTTCCTTTGA 6721 AAAACACGAT GATAAGCTTG CCACAACCCA CAAGGAGACG ACCTTCCATG ACCGAGTACA 6781 AGCCCACGGT GCGCCTCGCC ACCCGCGACG ACGTCCCCCG GGCCGTACGC ACCCTCGCCG 6841 CCGCGTTCGC CGACTACCCC GCCACGCGCC ACACCGTCGA CCCGGACCGC CACATCGAGC 6901 GGGTCACCGA GCTGCAAGAA CTCTTCCTCA CGCGCGTCGG GCTCGACATC GGCAAGGTGT 6961 GGGTCGCGGA CGACGGCGCC GCGGTGGCGG TCTGGACCAC GCCGGAGAGC GTCGAAGCGG 7021 GGGCGGTGTT CGCCGAGATC GGCCCGCGCA TGGCCGAGTT GAGCGGTTCC CGGCTGGCCG 7081 CGCAGCAACA GATGGAAGGC CTCCTGGCGC CGCACCGGCC CAAGGAGCCC GCGTGGTTCC 7141 TGGCCACCGT CGGCGTCTCG CCCGACCACC AGGGCAAGGG TCTGGGCAGC GCCGTCGTGC 7201 TCCCCGGAGT GGAGGCGGCC GAGCGCGCCG GGGTGCCCGC CTTCCTGGAG ACCTCCGCGC 7261 CCCGCAACCT CCCCTTCTAC GAGCGGCTCG GCTTCACCGT CACCGCCGAC GTCGAGGTGC 7321 CCGAAGGACC GCGCACCTGG TGCATGACCC GCAAGCCCGG TGCCTGATAA TAGGCGGCCG 7381 CTCGAGACCT AGAAAAACAT GGAGCAATCA CAAGTAGCAA TACAGCAGCT ACCAATGCTG 7441 ATTGTGCCTG GCTAGAAGCA CAAGAGGAGG AGGAGGTGGG TTTTCCAGTC ACACCTCAGG 7501 TACCTTTAAG ACCAATGACT TACAAGGCAG CTGTAGATCT TAGCCACTTT TTAAAAGAAA 7561 AGGGGGGACT GGAAGGGCTA ATTCACTCCC AACGAAGACA AGATCTGCTT TTTGCTTGTA 7621 CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT CTCTGGCTAA CTAGGGAACC 7681 CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT GCCCGTCTGT 7741 TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA GTCAGTGTGG AAAATCTCTA 7801 GCAGTAGTAG TTCATGTCAT CTTATTATTC AGTATTTATA ACTTGCAAAG AAATGAATAT 7861 CAGAGAGTGA GAGGCCTTGA CATTATAATA GATTTAGCAG GAATTGAACT AGGAGTGGAG 7921 CACACAGGCA AAGTTCTAGA GCTCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC 7981 CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG 8041 TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC 8101 TGGGGGGTGG GGGTGGGGCA GGACAGCAAG GGGGGAGGAT TGGGAAGACA ATAGCAGGCA 8161 TGCTGGGGAT GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCAGCT GGGGGCGCGC 8221 CCCTCGAGGC CGCCATGGTC ATAGCTGTTT GACGTCAGGT GGCACTTTTC GGGGAAATGT 8281 GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCACGAG 8341 ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA 8401 TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC 8461 AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT 8521 CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC 8581 AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG 8641 GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC 8701 AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT 8761 AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA 8821 GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC 8881 GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC 8941 AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT 9001 AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC 9061 TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC 9121 AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA 9181 GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA 9241 TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT 9301 TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA 9361 ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG 9421 AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC 9481 GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG 9541 CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA 9601 GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC 9661 CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGCG 9721 CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC 9781 ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA 9841 AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT 9901 CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG 9961 CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG 10021 GCCTTTTTAC GGTTCTGGCC TTTTGCA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???