pT3-EF1a-MYC-U6-Ggh-shRNA1-IRES-luc plasmid

PVT96115

pT3-EF1a-MYC-U6-Ggh-shRNA1-IRES-luc plasmid Information

Plasmid name: pT3-EF1a-MYC-U6-Ggh(mouse)-shRNA1-IRES-luc
Alias: GCTGCATCCTATGTGAAGTAT
Gene lenght:
Host: Mammalian cells
Use(s): Interference silence
Fragment type: shRNA
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc
Promoter: U6
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pT3-EF1a-MYC-U6-Ggh-shRNA1-IRES-luc plasmid Description

pT3-EF1a-MYC-U6-Ggh-shRNA1-IRES-luc This is a plasmid suitable for interference silencing in mammalian cells.

pT3-EF1a-MYC-U6-Ggh-shRNA1-IRES-luc plasmid Sequence

1 CTAGCTCGAG ATCTTAATTA AACTAGTATC GATATAACTT CGTATAATGT ATGCTATACG 61 AAGTTATATG TACGGGCCAG ATATACGCGT TATCGATCAC GAGACTAGCC TCGAGCGGCC 121 GCCCCCTTCA CCGAGGGCCT ATTTCCCATG ATTCCTTCAT ATTTGCATAT ACGATACAAG 181 GCTGTTAGAG AGATAATTGG AATTAATTTG ACTGTAAACA CAAAGATATT AGTACAAAAT 241 ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG TTTTAAAATT ATGTTTTAAA 301 ATGGACTATC ATATGCTTAC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC 361 TTGTGGAAAG GACGAAACAC CGGGCTGCAT CCTATGTGAA GTATctcgag ATACTTCACA 421 TAGGATGCAG CTTTTTGAAT TCTCGACCTC GAGACAAATG GCAGTATTCA TCTGACATTG 481 ATTATTGACT AGGCTTTTGC AAAAAGCTTT GCAAAGATGG ATAAAGTTTT AAACAGAGAG 541 GAATCTTTGC AGCTAATGGA CCTTCTAGGT CTTGAAAGGA GTGGGAATTG GCTCCGGTGC 601 CCGTCAGTGG GCAGAGCGCA CATCGCCCAC AGTCCCCGAG AAGTTGGGGG GAGGGGTCGG 661 CAATTGAACC GGTGCCTAGA GAAGGTGGCG CGGGGTAAAC TGGGAAAGTG ATGTCGTGTA 721 CTGGCTCCGC CTTTTTCCCG AGGGTGGGGG AGAACCGTAT ATAAGTGCAG TAGTCGCCGT 781 GAACGTTCTT TTTCGCAACG GGTTTGCCGC CAGAACACAG GTAAGTGCCG TGTGTGGTTC 841 CCGCGGGCCT GGCCTCTTTA CGGGTTATGG CCCTTGCGTG CCTTGAATTA CTTCCACCTG 901 GCTGCAGTAC GTGATTCTTG ATCCCGAGCT TCGGGTTGGA AGTGGGTGGG AGAGTTCGAG 961 GCCTTGCGCT TAAGGAGCCC CTTCGCCTCG TGCTTGAGTT GAGGCCTGGC CTGGGCGCTG 1021 GGGCCGCCGC GTGCGAATCT GGTGGCACCT TCGCGCCTGT CTCGCTGCTT TCGATAAGTC 1081 TCTAGCCATT TAAAATTTTT GATGACCTGC TGCGACGCTT TTTTTCTGGC AAGATAGTCT 1141 TGTAAATGCG GGCCAAGATC TGCACACTGG TATTTCGGTT TTTGGGGCCG CGGGCGGCGA 1201 CGGGGCCCGT GCGTCCCAGC GCACATGTTC GGCGAGGCGG GGCCTGCGAG CGCGGCCACC 1261 GAGAATCGGA CGGGGGTAGT CTCAAGCTGG CCGGCCTGCT CTGGTGCCTG GCCTCGCGCC 1321 GCCGTGTATC GCCCCGCCCT GGGCGGCAAG GCTGGCCCGG TCGGCACCAG TTGCGTGAGC 1381 GGAAAGATGG CCGCTTCCCG GCCCTGCTGC AGGGAGCTCA AAATGGAGGA CGCGGCGCTC 1441 GGGAGAGCGG GCGGGTGAGT CACCCACACA AAGGAAAAGG GCCTTTCCGT CCTCAGCCGT 1501 CGCTTCATGT GACTCCACGG AGTACCGGGC GCCGTCCAGG CACCTCGATT AGTTCTCGAG 1561 CTTTTGGAGT ACGTCGTCTT TAGGTTGGGG GGAGGGGTTT TATGCGATGG AGTTTCCCCA 1621 CACTGAGTGG GTGGAGACTG AAGTTAGGCC AGCTTGGCAC TTGATGTAAT TCTCCTTGGA 1681 ATTTGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG TGGTTCAAAG 1741 TTTTTTTCTT CCATTTCAGG TGTCGTGAGG AATTAGCTTG GTACTAATAC GACTCACTAT 1801 AGGGAGACCC AAGCTGGCTA GGTAAGCTTG GTACCGAGCT CGGATCCACT AGTCCAGTGT 1861 GGTGGAATTC TGCAGATATC AACAAGTTTG TACAAAAAAG CAGGCTTCGC CACCATGCCC 1921 CTCAACGTTA GCTTCACCAA CAGGAACTAT GACCTCGACT ACGACTCGGT GCAGCCGTAT 1981 TTCTACTGCG ACGAGGAGGA GAACTTCTAC CAGCAGCAGC AGCAGAGCGA GCTGCAGCCC 2041 CCGGCGCCCA GCGAGGATAT CTGGAAGAAA TTCGAGCTGC TGCCCACCCC GCCCCTGTCC 2101 CCTAGCCGCC GCTCCGGGCT CTGCTCGCCC TCCTACGTTG CGGTCACACC CTTCTCCCTT 2161 CGGGGAGACA ACGACGGCGG TGGCGGGAGC TTCTCCACGG CCGACCAGCT GGAGATGGTG 2221 ACCGAGCTGC TGGGAGGAGA CATGGTGAAC CAGAGTTTCA TCTGCGACCC GGACGACGAG 2281 ACCTTCATCA AAAACATCAT CATCCAGGAC TGTATGTGGA GCGGCTTCTC GGCCGCCGCC 2341 AAGCTCGTCT CAGAGAAGCT GGCCTCCTAC CAGGCTGCGC GCAAAGACAG CGGCAGCCCG 2401 AACCCCGCCC GCGGCCACAG CGTCTGCTCC ACCTCCAGCT TGTACCTGCA GGATCTGAGC 2461 GCCGCCGCCT CAGAGTGCAT CGACCCCTCG GTGGTCTTCC CCTACCCTCT CAACGACAGC 2521 AGCTCGCCCA AGTCCTGCGC CTCGCAAGAC TCCAGCGCCT TCTCTCCGTC CTCGGATTCT 2581 CTGCTCTCCT CGACGGAGTC CTCCCCGCAG GGCAGCCCCG AGCCCCTGGT GCTCCATGAG 2641 GAGACACCGC CCACCACCAG CAGCGACTCT GAGGAGGAAC AAGAAGATGA GGAAGAAATC 2701 GATGTTGTTT CTGTGGAAAA GAGGCAGGCT CCTGGCAAAA GGTCAGAGTC TGGATCACCT 2761 TCTGCTGGAG GCCACAGCAA ACCTCCTCAC AGCCCACTGG TCCTCAAGAG GTGCCACGTC 2821 TCCACACATC AGCACAACTA CGCAGCGCCT CCCTCCACTC GGAAGGACTA TCCTGCTGCC 2881 AAGAGGGTCA AGTTGGACAG TGTCAGAGTC CTGAGACAGA TCAGCAACAA CCGAAAATGC 2941 ACCAGCCCCA GGTCCTCGGA CACCGAGGAG AATGTCAAGA GGCGAACACA CAACGTCTTG 3001 GAGCGCCAGA GGAGGAACGA GCTAAAACGG AGCTTTTTTG CCCTGCGTGA CCAGATCCCG 3061 GAGTTGGAAA ACAATGAAAA GGCCCCCAAG GTAGTTATCC TTAAAAAAGC CACAGCATAC 3121 ATCCTGTCCG TCCAAGCAGA GGAGCAAAAG CTCATTTCTG AAGAGGACTT GTTGCGGAAA 3181 CGACGAGAAC AGTTGAAACA CAAACTTGAA CAGCTACGGA ACTCTTGTGC GGACCCAGCT 3241 TTCTTGTACA AAGTGGTTGA TATCCAGCAC AGTGGCGGCC GCTCGAGTCT AGAGGGCCCG 3301 CGGTTCGAAG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GCGTACCGGT 3361 TAGTAATGAG TTTCCCCCCC CCCCTAACGT TACTGGCCGA AGCCGCTTGG AATAAGGCCG 3421 GTGTGCGTTT GTCTATATGT TATTTTCCAC CATATTGCCG TCTTTTGGCA ATGTGAGGGC 3481 CCGGAAACCT GGCCCTGTCT TCTTGACGAG CATTCCTAGG GGTCTTTCCC CTCTCGCCAA 3541 AGGAATGCAA GGTCTGTTGA ATGTCGTGAA GGAAGCAGTT CCTCTGGAAG CTTCTTGAAG 3601 ACAAACAACG TCTGTAGCGA CCCTTTGCAG GCAGCGGAAC CCCCCACCTG GCGACAGGTG 3661 CCTCTGCGGC CAAAAGCCAC GTGTATAAGA TACACCTGCA AAGGCGGCAC AACCCCAGTG 3721 CCACGTTGTG AGTTGGATAG TTGTGGAAAG AGTCAAATGG CTCTCCTCAA GCGTATTCAA 3781 CAAGGGGCTG AAGGATGCCC AGAAGGTACC CCATTGTATG GGATCTGATC TGGGGCCTCG 3841 GTGCACATGC TTTACATGTG TTTAGTCGAG GTTAAAAAAA CGTCTAGGCC CCCCGAACCA 3901 CGGGGACGTG GTTTTCCTTT GAAAAACACG ATGATAATAT GGCCACAACC ATGGAAGACG 3961 CCAAAAACAT AAAGAAAGGC CCGGCGCCAT TCTATCCGCT GGAAGATGGA ACCGCTGGAG 4021 AGCAACTGCA TAAGGCTATG AAGAGATACG CCCTGGTTCC TGGAACAATT GCTTTTACAG 4081 ATGCACATAT CGAGGTGGAC ATCACTTACG CTGAGTACTT CGAAATGTCC GTTCGGTTGG 4141 CAGAAGCTAT GAAACGATAT GGGCTGAATA CAAATCACAG AATCGTCGTA TGCAGTGAAA 4201 ACTCTCTTCA ATTCTTTATG CCGGTGTTGG GCGCGTTATT TATCGGAGTT GCAGTTGCGC 4261 CCGCGAACGA CATTTATAAT GAACGTGAAT TGCTCAACAG TATGGGCATT TCGCAGCCTA 4321 CCGTGGTGTT CGTTTCCAAA AAGGGGTTGC AAAAAATTTT GAACGTGCAA AAAAAGCTCC 4381 CAATCATCCA AAAAATTATT ATCATGGATT CTAAAACGGA TTACCAGGGA TTTCAGTCGA 4441 TGTACACGTT CGTCACATCT CATCTACCTC CCGGTTTTAA TGAATACGAT TTTGTGCCAG 4501 AGTCCTTCGA TAGGGACAAG ACAATTGCAC TGATCATGAA CTCCTCTGGA TCTACTGGTC 4561 TGCCTAAAGG TGTCGCTCTG CCTCATAGAA CTGCCTGCGT GAGATTCTCG CATGCCAGAG 4621 ATCCTATTTT TGGCAATCAA ATCATTCCGG ATACTGCGAT TTTAAGTGTT GTTCCATTCC 4681 ATCACGGTTT TGGAATGTTT ACTACACTCG GATATTTGAT ATGTGGATTT CGAGTCGTCT 4741 TAATGTATAG ATTTGAAGAA GAGCTGTTTC TGAGGAGCCT TCAGGATTAC AAGATTCAAA 4801 GTGCGCTGCT GGTGCCAACC CTATTCTCCT TCTTCGCCAA AAGCACTCTG ATTGACAAAT 4861 ACGATTTATC TAATTTACAC GAAATTGCTT CTGGTGGCGC TCCCCTCTCT AAGGAAGTCG 4921 GGGAAGCGGT TGCCAAGAGG TTCCATCTGC CAGGTATCAG GCAAGGATAT GGGCTCACTG 4981 AGACTACATC AGCTATTCTG ATTACACCCG AGGGGGATGA TAAACCGGGC GCGGTCGGTA 5041 AAGTTGTTCC ATTTTTTGAA GCGAAGGTTG TGGATCTGGA TACCGGGAAA ACGCTGGGCG 5101 TTAATCAAAG AGGCGAACTG TGTGTGAGAG GTCCTATGAT TATGTCCGGT TATGTAAACA 5161 ATCCGGAAGC GACCAACGCC TTGATTGACA AGGATGGATG GCTACATTCT GGAGACATAG 5221 CTTACTGGGA CGAAGACGAA CACTTCTTCA TCGTTGACCG CCTGAAGTCT CTGATTAAGT 5281 ACAAAGGCTA TCAGGTGGCT CCCGCTGAAT TGGAATCCAT CTTGCTCCAA CACCCCAACA 5341 TCTTCGACGC AGGTGTCGCA GGTCTTCCCG ACGATGACGC CGGTGAACTT CCCGCCGCCG 5401 TTGTTGTTTT GGAGCACGGA AAGACGATGA CGGAAAAAGA GATCGTGGAT TACGTCGCCA 5461 GTCAAGTAAC AACCGCGAAA AAGTTGCGCG GAGGAGTTGT GTTTGTGGAC GAAGTACCGA 5521 AAGGTCTTAC CGGAAAACTC GACGCAAGAA AAATCAGAGA GATCCTCATA AAGGCCAAGA 5581 AGGGCGGAAA GATCGCCGTG TAATTCTAGG ATCCGTCGAC CTGCAGCCAA GCTTATCGAT 5641 AAACCCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT 5701 CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG 5761 AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC 5821 AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAT GCGGTGGGCT 5881 CTATGGCTTC TGAGGCGGAA AGAACCAGCT GGGGCTCTAG GGGGTATCCC CACGCGCCCT 5941 GTAGCGGCGC ATTAAATAAC TTCGTATAAT GTATGCTATA CGAAGTTATA TCGTATGCAT 6001 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 6061 ATTCCACAGA ATTCCTTGAA ATACATCCAC AGGTACACCT CCAATTGACT CAAATGATGT 6121 CAATTAGTCT ATCAGAAGCT TCTAAAGCCA TGACATCATT TTCTGGAATT TTCCAAGCTG 6181 TTTAAAGGCA CAGTCAACTT AGTGTATGTA AACTTCTGAC CCACTGGAAT TGTGATACAG 6241 TGAATTATAA GTGAAATAAT CTGTCTGTAA ACAATTGTTG GAAAAATGAC TTGTGTCATG 6301 CACAAAGTAG ATGTCCTAAC TGACTTGCCA AAACTATTGT TTGTTAACAA GAAATTTGTG 6361 GAGTAGTTGA AAAACGAGTT TTAATGACTC CAACTTAAGT GTATGTAAAC TTCCGACTTC 6421 AACTGTAGGG TACCGAGCTC GAATTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA 6481 AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT 6541 AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA 6601 TGGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATGG 6661 TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGCCCCG ACACCCGCCA 6721 ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT 6781 GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG 6841 AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT 6901 TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT 6961 TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA 7021 TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT 7081 TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT 7141 GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG 7201 ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG 7261 CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA 7321 CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT 7381 GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC 7441 AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG 7501 GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC 7561 GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT 7621 GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA 7681 GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT 7741 GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC 7801 TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA 7861 CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC 7921 TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG 7981 ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG 8041 TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC 8101 TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG 8161 CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT 8221 CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC 8281 CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC 8341 GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT 8401 TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT 8461 GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC 8521 GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT 8581 TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA 8641 GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT 8701 TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT 8761 ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG 8821 TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG 8881 CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC 8941 AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT 9001 CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT 9061 GACCATGATT ACGCCAAGCT TGCATGCCTG CAGGTCGACT CTAGACCTAT ACAGTTGAAG 9121 TCGGAAGTTT ACATACACTT AAGTTGGAGT CATTAAAACT CGTTTTTCAA CTACTCCACA 9181 AATTTCTTGT TAACAAACAA TAGTTTTGGC AAGTCAGTTA GGACATCTAC TTTGTGCATG 9241 ACACAAGTCA TTTTTCCAAC AATTGTTTAC AGACAGATTA TTTCACTTAT AATTCACTGT 9301 ATCACAATTC CAGTGGGTCA GAAGTTTACA TACACTAAGT TGACTGTGCC TTTAAACAGC 9361 TTGGAAAATT CCAGAAAATG ATGTCATGGC TTTAGAAGCT TCTGATAGAC TAATTGACAT 9421 CATTTGAGTC AATTGGAGGT GTACCTGTGG ATGTATTTCA AGGGGATCCA GACATGATAA 9481 GATACATTGA TGAGTTTGGA CAAACCACAA CTAGAATGCA GCGGCCG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???