pT7-7
PVT10591 Packing 2ug
pT7-7 Description
PT7-7 is an Escherichia coli pET expression plasmid.
pT7-7 Cloning site
pT7-7 Sequence
LOCUS Exported 2473 bp ds-DNA circular SYN 19-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..2473
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 25..87
/label=MCS
RBS 92..114
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
promoter complement(146..164)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 337..477
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(663..1251)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 1467..1571
/gene="bla"
/label=AmpR promoter
CDS 1572..2432
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ORIGIN
1 aattctcatg tttgacagct tatcatcgat aagcttgggc tgcaggtcga ctctagagga
61 tccccgggcg cgaattctag ccatatgtat atctccttct taaagttaaa caaaattatt
121 tctagaggga aaccgttgtg gtctccctat agtgagtcgt attaatttcg aagtctatca
181 gaagttcgaa tcgctgggcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
241 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
301 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
361 cgatagcgga gtgtatatac tggcttaact atgcggcatc agagcagatt gtactgagag
421 tgcaccatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
481 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
541 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
601 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
661 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
721 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
781 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
841 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
901 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
961 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
1021 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
1081 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
1141 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
1201 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
1261 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
1321 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taattcttga agacgaaagg
1381 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
1441 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
1501 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
1561 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
1621 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
1681 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
1741 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
1801 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
1861 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
1921 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
1981 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
2041 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
2101 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
2161 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
2221 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
2281 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
2341 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
2401 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
2461 tttagattga ttt
//
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Product is for research use only!
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