pT7-6×His-HMGB1-EGFP-6×His plasmid
PVT99303
pT7-6×His-HMGB1-EGFP-6×His plasmid Information
Plasmid name: pT7-6×His-HMGB1(human)-EGFP-6×His
Alias: NM_001313893.1;HMG-1; HMG1; HMG3; SBP-1
Gene lenght: 645bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling: Green
Promoter: T7
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: T7
Reverse primer:
Copy number: High
Induction:
pT7-6×His-HMGB1-EGFP-6×His plasmid Description
pT7-6×His-HMGB1-EGFP-6×His is a plasmid suitable for protein expression in Escherichia coli.
pT7-6×His-HMGB1-EGFP-6×His plasmid Sequence
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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???
Technical Principle & Methodology
The quality assurance and experimental performance of this biological reagent are rigorously optimized and standardized according to its specific technical pathway:
Recombinant DNA Molecular Cloning and Expression Vector Architecture
(重组DNA分子克隆与表达载体工程构建技术). This ensures maximum reproducibility across clinical research and scientific workflows.