pT7-GST-6×His-APLN plasmid

PVT100491

pT7-GST-6×His-APLN plasmid Information

Plasmid name: pT7-GST-6×His-APLN(human)
Alias: NM_017413.5;APEL; XNPEP2
Gene lenght: 234bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: T7
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: GST-F2
Reverse primer:
Copy number: Low
Induction: IPTG Induction

pT7-GST-6×His-APLN plasmid Description

pT7-GST-6×His-APLN is a plasmid suitable for protein expression in Escherichia coli.

pT7-GST-6×His-APLN plasmid Sequence

1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg gttccgcgtg gatccccgCA TCATCATCAT CATCATgaat 961 tcatgaatct gcggctctgc gtgcaggcgc tcctgctgct ctggctctcc ttgaccgcgg 1021 tgtgtggagg gtccctgatg ccgcttcccg atgggaatgg gctggaagac ggcaatgtcc 1081 gccacctggt gcagcccaga gggtcaagga atgggccagg gccctggcag ggaggtcgga 1141 ggaaattccg ccgccagcgg ccccgcctct cccataaggg acccatgcct ttctgactcg 1201 agcggccgca tcgtgactga ctgacgatct gcctcgcgcg tttcggtgat gacggtgaaa 1261 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga 1321 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga 1381 cccagtcacg tagcgatagc ggagtgtata attcttgaag acgaaagggc ctcgtgatac 1441 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt 1501 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 1561 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta 1621 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg 1681 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac 1741 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg 1801 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc 1861 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg 1921 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat 1981 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg 2041 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg 2101 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc 2161 ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt 2221 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct 2281 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc 2341 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca 2401 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct 2461 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt 2521 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 2581 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 2641 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 2701 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 2761 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag 2821 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 2881 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 2941 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 3001 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 3061 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 3121 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 3181 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 3241 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 3301 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg 3361 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag 3421 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcataaatt 3481 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga 3541 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg 3601 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa 3661 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac 3721 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc 3781 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg 3841 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc 3901 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca 3961 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga 4021 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc 4081 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg 4141 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag 4201 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga 4261 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa 4321 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg 4381 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc 4441 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga 4501 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata 4561 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 4621 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag 4681 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga 4741 taacaatttc acacaggaaa cagctatgac catgattacg gattcactgg ccgtcgtttt 4801 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc 4861 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt 4921 gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga 4981 aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct caaactggca 5041 gatgcacggt tacgatgcgc ccatctacac caacgtaacc tatcccatta cggtcaatcc 5101 gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta atgttgatga 5161 aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttggaa tt

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???