pTac-EGFP-NOLC1-6×His plasmid

PVT96319

pTac-EGFP-NOLC1-6×His plasmid Information

Plasmid name: pTac-EGFP-NOLC1(human)-6×His
Alias: NM_001284388.2;NOPP130; NOPP140; NS5ATP13; P130; Srp40
Gene lenght: 2127bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: Tac
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: High
Induction:

pTac-EGFP-NOLC1-6×His plasmid Description

pTac-EGFP-NOLC1-6×His is a plasmid suitable for protein expression in Escherichia coli.

pTac-EGFP-NOLC1-6×His plasmid Sequence

1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca 301 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg 361 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc 421 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct 481 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc 541 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt 601 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg 661 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg 721 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg 781 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc 841 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga 901 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg 961 acgagctgta caagGAATTC ggtggaggcg gatcaggagg cggtggatct atggcggacg 1021 ccggcattcg ccgcgtggtt cccagcgacc tgtatcccct cgtgctcggc ttcctgcgcg 1081 ataaccaact ctcagaggtg gccaataagt tcgccaaagc gacaggagct acacagcagg 1141 atgccaatgc ctcttccctc ttagacatct atagcttctg gctcaagtct gccaaggtcc 1201 cagagcgaaa gttacaggca aatggaccag tggctaagaa agctaagaag aaggcctcat 1261 ccagtgacag tgaggacagc agcgaggagg aggaggaagt tcaagggcct ccagcaaaga 1321 aggctgctgt acctgccaag cgagtcggtc tgcctcctgg gaaggctgca gccaaagcat 1381 cagagagtag cagcagtgaa gagtccagtg atgatgatga tgaggaggac caaaagaaac 1441 agcctgtcca gaagggagtt aagccccaag ccaaggcagc caaagctcct cctaagaagg 1501 ccaagagctc tgattctgat tctgactcaa gctccgagga tgagccacca aagaaccaga 1561 agccaaagat aacacctgtg acagttaaag ctcagactaa agcccctccc aaaccagctc 1621 gagcagcacc taaaatagcc aatggtaaag cagccagtag cagcagtagc agcagcagca 1681 gcagtagcag tgatgactca gaggaggaga aggcagcagc cacccccaag aaggtctgga 1741 ccataacttc tgtcagggca gagactgtac ctaaaaagca agttgtggcc aaggccccag 1801 tgaaagcagc taccacccct acccggaaga gttctagcag tgaggattcc tccagtgacg 1861 aggaagagga gcaaaaaaaa cccatgaaaa ataaaccagg tccctacagt tcagtccccc 1921 cgccttctgc tcccccacca aagaagtctc tgggaaccca gcctcccaag aaggctgtgg 1981 agaagcagca gcctgtggaa agcagtgaag acagcagtga tgagtctgat tcaagttctg 2041 aagaagagaa gaaaccccca actaaggcag tagtctctaa agcaaccact aaaccacctc 2101 cagcaaagaa agcagcagag agctcttcag acagctcaga ctctgacagc tctgaggatg 2161 atgaagctcc ttctaagcca gctggtacca ccaagaattc ttcaaataag ccagctgtca 2221 ccaccaagtc acctgcagtg aagccagctg cagcccccaa gcaacctgtg ggcggtggcc 2281 agaagcttct gacgagaaag gctgacagca gctccagtga ggaagagagc agctccagtg 2341 aggaggagaa gacaaagaag atggtggcca ccactaagcc caaggcgact gccaaagcag 2401 ctctatctct gcctgccaag caggctcctc agggtagtag ggacagcagc tctgattcag 2461 acagctccag cagtgaggag gaggaagaga agacatctaa gtctgcagtt aagaagaagc 2521 cacagaaggt agcaggaggt gcagcccctt ccaagccagc ctctgcaaag aaaggaaagg 2581 ctgagagcag caacagttct tcttctgatg actccagtga ggaagaggaa gagaagctca 2641 agggcaaggg ctctccaaga ccacaagccc ccaaggccaa tggcacctct gcactgactg 2701 cccagaatgg aaaagcagct aagaacagtg aggaggagga agaagaaaag aaaaaggcgg 2761 cagtggtagt ttccaaatca ggttcattaa agaagcggaa gcagaatgag gctgccaagg 2821 aggcagagac tcctcaggcc aagaagataa agcttcagac ccctaacaca tttccaaaaa 2881 ggaagaaagg agaaaaaagg gcatcatccc cattccgaag ggtcagggag gaggaaattg 2941 aggtggattc acgagttgcg gacaactcct ttgatgccaa gcgaggtgca gccggagact 3001 ggggagagcg agccaatcag gttttgaagt tcaccaaagg caagtccttt cggcatgaga 3061 aaaccaagaa gaagcggggc agctaccggg gaggctcaat ctctgtccag gtcaattcta 3121 ttaagtttga cagcgagCAT CACCATCATC ACCATtagcg gccgcatcgt gactgactga 3181 cgatctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg 3241 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt 3301 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag 3361 tgtataattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca 3421 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc 3481 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 3541 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 3601 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 3661 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 3721 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 3781 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac 3841 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 3901 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 3961 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 4021 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 4081 aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc 4141 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 4201 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 4261 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 4321 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 4381 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 4441 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 4501 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 4561 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 4621 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 4681 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 4741 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 4801 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 4861 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 4921 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 4981 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 5041 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 5101 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 5161 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 5221 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 5281 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 5341 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc 5401 ttacgcatct gtgcggtatt tcacaccgca taaattccga caccatcgaa tggtgcaaaa 5461 cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga 5521 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc 5581 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga 5641 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt 5701 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg 5761 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa 5821 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc 5881 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta 5941 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct 6001 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa 6061 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc 6121 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg 6181 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga 6241 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc 6301 tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt 6361 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg 6421 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct 6481 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt 6541 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag 6601 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg 6661 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc 6721 tatgaccatg attacggatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc 6781 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag 6841 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg 6901 ctttgcctgg tttccggcac cagaagcggt gccggaaagc tggctggagt gcgatcttcc 6961 tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg cacggttacg atgcgcccat 7021 ctacaccaac gtaacctatc ccattacggt caatccgccg tttgttccca cggagaatcc 7081 gacgggttgt tactcgctca catttaatgt tgatgaaagc tggctacagg aaggccagac 7141 gcgaattatt tttgatggcg ttggaatt

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???