pTac-GST-EFNA1 plasmid

PVT112075

pTac-GST-EFNA1 plasmid Information

Plasmid name: pTac-GST-EFNA1(human)
Alias: NM_004428.3;B61; ECKLG; EFL1; EPLG1; GMAN; LERK-1; LERK1; TNFAIP4
Gene lenght: 618
Host: E.coli
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: Tac
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: GST-F1
Reverse primer:
Copy number: Low
Induction: IPTG Induction

pTac-GST-EFNA1 plasmid Description

pTac-GST-EFNA1 Plasmid suitable for gene expression in Escherichia coli.

pTac-GST-EFNA1 plasmid Sequence

1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg gttccgcgtg gatccatgga gttcctctgg gcccctctct 961 tgggtctgtg ctgcagtctg gccgctgctg atcgccacac cgtcttctgg aacagttcaa 1021 atcccaagtt ccggaatgag gactacacca tacatgtgca gctgaatgac tacgtggaca 1081 tcatctgtcc gcactatgaa gatcactctg tggcagacgc tgccatggag cagtacatac 1141 tgtacctggt ggagcatgag gagtaccagc tgtgccagcc ccagtccaag gaccaagtcc 1201 gctggcagtg caaccggccc agtgccaagc atggcccgga gaagctgtct gagaagttcc 1261 agcgcttcac acctttcacc ctgggcaagg agttcaaaga aggacacagc tactactaca 1321 tctccaaacc catccaccag catgaagacc gctgcttgag gttgaaggtg actgtcagtg 1381 gcaaaatcac tcacagtcct caggcccatg acaatccaca ggagaagaga cttgcagcag 1441 atgacccaga ggtgcgggtt ctacatagca tcggtcacag tgctgcccca cgcctcttcc 1501 cacttgcctg gactgtgctg ctccttccac ttctgctgct gcaaaccccg tgactcgagc 1561 ggccgcatcg tgactgactg acgatctgcc tcgcgcgttt cggtgatgac ggtgaaaacc 1621 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca 1681 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc 1741 agtcacgtag cgatagcgga gtgtataatt cttgaagacg aaagggcctc gtgatacgcc 1801 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc 1861 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 1921 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 1981 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 2041 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 2101 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 2161 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg 2221 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 2281 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 2341 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 2401 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 2461 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 2521 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 2581 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 2641 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 2701 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 2761 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 2821 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 2881 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 2941 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 3001 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 3061 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 3121 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc 3181 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 3241 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 3301 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 3361 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 3421 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 3481 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 3541 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 3601 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 3661 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 3721 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 3781 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc ataaattccg 3841 acaccatcga atggtgcaaa acctttcgcg gtatggcatg atagcgcccg gaagagagtc 3901 aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg 3961 tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc 4021 gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac 4081 aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg 4141 cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg 4201 tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc 4261 tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg 4321 ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac 4381 ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg 4441 tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc 4501 gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg 4561 aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg 4621 agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc 4681 gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg 4741 ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc 4801 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg 4861 gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc 4921 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 4981 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca 5041 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa 5101 caatttcaca caggaaacag ctatgaccat gattacggat tcactggccg tcgttttaca 5161 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 5221 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 5281 cagcctgaat ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag 5341 ctggctggag tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa actggcagat 5401 gcacggttac gatgcgccca tctacaccaa cgtaacctat cccattacgg tcaatccgcc 5461 gtttgttccc acggagaatc cgacgggttg ttactcgctc acatttaatg ttgatgaaag 5521 ctggctacag gaaggccaga cgcgaattat ttttgatggc gttggaatt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.