pTac-GST-SNW1 plasmid

PVT91281

pTac-GST-SNW1 plasmid Information

Plasmid name: pTac-GST-SNW1(human)
Alias: NM_012245;Bx42; FUN20; NCOA-62; Prp45; PRPF45; SKIIP;SKIP; SKIP1;
Gene lenght: 1608bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: Tac
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature:
Vector backbone:
Forward primer: GST-F2
Reverse primer:
Copy number: High
Induction: IPTG Induction

pTac-GST-SNW1 plasmid Description

pTac-GST-SNW1 is a plasmid suitable for protein expression in Escherichia coli.

pTac-GST-SNW1 plasmid Sequence

1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg gttccgcgtg gatccatggc gctcaccagc tttttacctg 961 cacctactca gctatctcag gaccagcttg aggctgaaga aaaggcaaga tcccagagat 1021 cacggcagac ctcactggtc tcctcccgaa gagaacctcc cccgtacgga taccggaaag 1081 gctggatacc tcggttatta gaggattttg gagatggagg tgcttttcca gagatccatg 1141 tggcccagta tccactggat atgggacgaa agaaaaaaat gtcgaatgcg ctggccattc 1201 aggtggattc tgaaggaaaa attaaatatg atgcaattgc tcgacaagga cagtcaaaag 1261 acaaggtcat ttatagcaaa tacactgacc tggttccaaa ggaggttatg aatgcagatg 1321 atccagacct gcaaaggccc gatgaagaag ctattaaaga gataacagaa aagacaagag 1381 tagccttaga aaaatctgta tcacagaagg tcgccgcagc catgccagtt cgagcagctg 1441 acaaattggc tcctgctcag tatatccgat acacaccatc tcagcaagga gtggcattca 1501 actctggagc taaacagagg gttattcgga tggtagaaat gcagaaagat ccaatggagc 1561 ctccaaggtt caagattaat aagaaaattc cccggggacc accttctcct cctgcgcctg 1621 tcatgcattc tcctagccga aagatgactg taaaggaaca acaagagtgg aagattcctc 1681 cttgtatttc taactggaaa aatgcaaagg gttatacaat tccattagac aaacgtctgg 1741 ctgctgatgg aagaggacta cagacagtac acataaatga aaatttcgcc aaattggcag 1801 aagccctcta cattgctgat cggaaggctc gtgaagctgt ggaaatgcgt gcccaagtag 1861 agagaaaaat ggctcagaaa gaaaaggaaa aacatgaaga gaaacttaga gaaatggccc 1921 agaaagccag ggagagaaga gctgggatca aaactcatgt ggaaaaagag gatggggagg 1981 cacgtgagag ggatgaaatc cggcatgaca ggcgaaaaga gagacagcat gaccggaatc 2041 tttccagggc agctcctgat aagaggtcga aacttcagag aaatgaaaat cgggatatca 2101 gtgaagttat tgctctcggt gttcctaatc ctcggacttc caatgaagtt cagtatgacc 2161 aaaggctctt caaccaatcc aagggtatgg acagtggatt tgcaggtgga gaagatgaaa 2221 tttataatgt ttatgatcaa gcctggagag gtggtaaaga tatggcccag agtatttata 2281 ggcccagtaa aaatctggac aaggacatgt atggtgatga cctagaagcc agaataaaga 2341 ccaacagatt tgttcccgac aaggagtttt ctggttcaga ccgtagacag agaggccgag 2401 aaggaccagt gcagtttgag gaagatcctt ttggtttgga caagtttttg gaagaagcca 2461 aacagcatgg tggctctaaa agaccctcag atagcagccg ccccaaggaa cacgagcatg 2521 aaggcaagaa gaggaggaag gaaTAGctcg agcggccgca tcgtgactga ctgacgatct 2581 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 2641 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 2701 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata 2761 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa 2821 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 2881 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 2941 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 3001 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 3061 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 3121 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 3181 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc 3241 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 3301 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 3361 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 3421 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 3481 taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac 3541 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 3601 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 3661 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 3721 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 3781 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 3841 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 3901 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 3961 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 4021 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 4081 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 4141 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 4201 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 4261 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 4321 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 4381 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 4441 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 4501 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 4561 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 4621 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 4681 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 4741 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc 4801 atctgtgcgg tatttcacac cgcataaatt ccgacaccat cgaatggtgc aaaacctttc 4861 gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag 4921 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg 4981 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg 5041 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga 5101 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta 5161 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg 5221 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca 5281 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc 5341 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg 5401 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc 5461 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat 5521 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt 5581 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg 5641 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg 5701 ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc 5761 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct 5821 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg 5881 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 5941 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac 6001 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg 6061 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac 6121 catgattacg gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt 6181 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga 6241 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc 6301 ctggtttccg gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc 6361 cgatactgtc gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac 6421 caacgtaacc tatcccatta cggtcaatcc gccgtttgtt cccacggaga atccgacggg 6481 ttgttactcg ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat 6541 tatttttgat ggcgttggaa tt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???