pTac-VHL-MBP plasmid

PVT91933

pTac-VHL-MBP plasmid Information

Plasmid name: pTac-VHL(human)-MBP
Alias: NM_000551.4;HRCA1; pVHL; RCA1; VHL1
Gene lenght: 639bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: T7
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: Tac-P
Reverse primer:
Copy number: High
Induction: IPTG Induction

pTac-VHL-MBP plasmid Description

pTac-VHL-MBP is a plasmid suitable for protein expression in Escherichia coli.

pTac-VHL-MBP plasmid Sequence

1 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga 61 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg 121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa 181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac 241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc 301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg 361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc 421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca 481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga 541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc 601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg 661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag 721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga 781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa 841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg 901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc 961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga 1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata 1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 1141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag 1201 gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg 1261 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg 1321 tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt 1381 ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga 1441 attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga 1501 gcacttcacc aacaaggacc atagcatatg ccccggaggg cggagaactg ggacgaggcc 1561 gaggtaggcg cggaggaggc aggcgtcgaa gagtacggcc ctgaagaaga cggcggggag 1621 gagtcgggcg ccgaggagtc cggcccggaa gagtccggcc cggaggaact gggcgccgag 1681 gaggagatgg aggccgggcg gccgcggccc gtgctgcgct cggtgaactc gcgcgagccc 1741 tcccaggtca tcttctgcaa tcgcagtccg cgcgtcgtgc tgcccgtatg gctcaacttc 1801 gacggcgagc cgcagcccta cccaacgctg ccgcctggca cgggccgccg catccacagc 1861 taccgaggtc acctttggct cttcagagat gcagggacac acgatgggct tctggttaac 1921 caaactgaat tatttgtgcc atctctcaat gttgacggac agcctatttt tgccaatatc 1981 acactgccag tgtatactct gaaagagcga tgcctccagg ttgtccggag cctagtcaag 2041 cctgagaatt acaggagact ggacatcgtc aggtcgctct acgaagatct ggaagaccac 2101 ccaaatgtgc agaaagacct ggagcggctg acacaggagc gcattgcaca tcaacggatg 2161 ggagatcata tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc 2221 tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc 2281 gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc 2341 cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg 2401 gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc 2461 gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt 2521 tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat 2581 aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc 2641 acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac 2701 gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt 2761 gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc 2821 tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac 2881 accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa 2941 ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca 3001 aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac 3061 aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt 3121 attgccgcca ccatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg 3181 tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact 3241 gtcgatgaag ccctgaaaga cgcgcagact tgaAAGCTTG Gcactggccg tcgttttaca 3301 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 3361 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 3421 cagcctgaat ggcgaatggc agcttggctg ttttggcgga tgagataaga ttttcagcct 3481 gatacagatt aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggcag 3541 tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga 3601 tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa 3661 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc 3721 tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt 3781 ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga 3841 cggatggcct ttttgcgttt ctacaaactc tttttgttta tttttctaaa tacattcaaa 3901 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa 3961 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct 4021 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg 4081 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg 4141 ccccgaagaa cgttctccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt 4201 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga 4261 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga 4321 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac 4381 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg 4441 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac 4501 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct 4561 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct 4621 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg 4681 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat 4741 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg 4801 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat 4861 tgatttaccc cggttgataa tcagaaaagc cccaaaaaca ggaagattgt ataagcaaat 4921 atttaaattg taaacgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc 4981 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc 5041 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac 5101 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca 5161 cccaaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg 5221 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag 5281 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc 5341 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt aaaaggatct aggtgaagat 5401 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 5461 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 5521 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 5581 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct 5641 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 5701 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 5761 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 5821 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 5881 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 5941 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 6001 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 6061 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 6121 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 6181 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 6241 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg 6301 tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta 6361 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc 6421 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag 6481 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg 6541 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgcag cgattcacag atgtctgcct 6601 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa 6661 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg 6721 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg 6781 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat 6841 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag 6901 atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg 6961 tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc 7021 atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta 7081 tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg 7141 acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgaa att

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???