pTK-Hmgcs1-promoter-Fluc plasmid

PVT106661

pTK-Hmgcs1-promoter-Fluc plasmid Information

Plasmid name: pTK-Hmgcs1(mouse)-promoter-Fluc
Alias:
Gene lenght: 1001
Host: Mammalian cells
Use(s):
Promoter detection
Fragment type: Promoter
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc
Promoter: TK,Hmgcs1
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: HSV TK-F2
Reverse primer:
Copy number: High
Induction:

pTK-Hmgcs1-promoter-Fluc plasmid Description

pTK-Hmgcs1-promoter-Fluc is a plasmid suitable for promoter detection in mammalian cells.

pTK-Hmgcs1-promoter-Fluc plasmid Sequence

1 GGCCTAACTG GCCGGTACct gagtctaaat gagtcttcgg acctcgcggg ggccgcttaa 61 gcggtggtta gggtttgtct gacgcggggg gagggggaag gaacgaaaca ctctcattcg 121 gaggcggctc ggggtttggt cttggtggcc acgggcacgc agaagagcgc cgcgatcctc 181 ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg taactggcgg 241 gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac 301 ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc catggggacc 361 gtatacgtgg acaggctctg gagcatccgc acgactgcgg tgatattacc ggagaccttc 421 tgcgggacga gccgggtcac gcggctgacg cggagcgtcc gttgggcgac aaacaccagg 481 acggggcaca ggtacactat cttgtcaccc ggaggcgcga gggactgcag gagcttcagg 541 gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg 601 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg 661 tcattggcga attcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg 721 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaaG 781 ACGAAGAGTT AGTAATACAG TTCTGAACCT CAGTCATTCA ACCTCAACAA AGCTAAGCAG 841 TACAGTTTCT GTTTGGACCA AGTGCCAAAA TAATAAATTA AAAAAAACAA ACAAACAAAA 901 AACGAAAACC AAAACAACAA CAAACACTGA TTTGTATAAA CCTTCCTTCT CAGACAAGCC 961 CTGCATCATT CTTATTCACT CCTATATCCT AAAATATCGT TTTTTAGGTT TACTTATTGT 1021 CTCAGTCGCA GTTTCTATAA AAGCCAAGGT AGTTTAAATT GGACACTTAA GAATACTTAC 1081 AAATTTAGAA ATTCTGGGTG AAGCATTATG CTAAAACTTG TGATGCAGAT GCAATTTGCT 1141 GCTCTGGAAA CTTAGAAAAG AGTCACGACT GAATTCAAAT CAAGTATTTC AAATATTTTA 1201 CAGGTGAAGC CGAGCTGTGG TTGCTAGGCA ACCTGACAGC CTCCCAAGCG GGGGACCAAC 1261 TCACCTCCCA CCCTTGACCA TCTCTCCCCA GGAGTGGGAC GCCGAGTCGC CATTGGTCTC 1321 TATCTCCTCT CACTGACCTT CAATTGGTCG GAGAACCTCT CACTCCGCTC CCGTTGGCTC 1381 TCGCCGTCTC TCGCCGTCCC GCCATTGGCA GGCTTGTTCT CGGGCCACCC TACGTCACCG 1441 TCTCACCCCA CTTCCCAGGT CCTCGGACTT TGTTCCCACC CCTTACCCCG CCCTTCCGGC 1501 AGCGAGGGAA AACCCTAGCG AGTCCCCGCC TCTCATTGGC TGAAAGCGGA TCGGGGGGCG 1561 GGCGCTCCCG AGCGACTCTG AGTCCCGGGA TTGGCAGAGG TAGCCGCTGT CCCGCCCTCT 1621 GTCCCCTCCC GGCGCTGCCC GAGTCGGGTG GGTCGGTGGC TATAAAGCTG CGGAGGGCGG 1681 GAGGCACAGT CTGCGGTCTC CTTGCTTTGC TCGTTCTTCT TCCAGGGTCT GATCCCCTTT 1741 GGGTGAGTGT CCCGCGCGGG GCCGGCCAGC CAAAGGCCGA aAGCTTGGCA ATCCGGTACT 1801 GTTGGTAAAG CCACCATGGA AGATGCCAAA AACATTAAGA AGGGCCCAGC GCCATTCTAC 1861 CCACTCGAAG ACGGGACCGC CGGCGAGCAG CTGCACAAAG CCATGAAGCG CTACGCCCTG 1921 GTGCCCGGCA CCATCGCCTT TACCGACGCA CATATCGAGG TGGACATTAC CTACGCCGAG 1981 TACTTCGAGA TGAGCGTTCG GCTGGCAGAA GCTATGAAGC GCTATGGGCT GAATACAAAC 2041 CATCGGATCG TGGTGTGCAG CGAGAATAGC TTGCAGTTCT TCATGCCCGT GTTGGGTGCC 2101 CTGTTCATCG GTGTGGCTGT GGCCCCAGCT AACGACATCT ACAACGAGCG CGAGCTGCTG 2161 AACAGCATGG GCATCAGCCA GCCCACCGTC GTATTCGTGA GCAAGAAAGG GCTGCAAAAG 2221 ATCCTCAACG TGCAAAAGAA GCTACCGATC ATACAAAAGA TCATCATCAT GGATAGCAAG 2281 ACCGACTACC AGGGCTTCCA AAGCATGTAC ACCTTCGTGA CTTCCCATTT GCCACCCGGC 2341 TTCAACGAGT ACGACTTCGT GCCCGAGAGC TTCGACCGGG ACAAAACCAT CGCCCTGATC 2401 ATGAACAGTA GTGGCAGTAC CGGATTGCCC AAGGGCGTAG CCCTACCGCA CCGCACCGCT 2461 TGTGTCCGAT TCAGTCATGC CCGCGACCCC ATCTTCGGCA ACCAGATCAT CCCCGACACC 2521 GCTATCCTCA GCGTGGTGCC ATTTCACCAC GGCTTCGGCA TGTTCACCAC GCTGGGCTAC 2581 TTGATCTGCG GCTTTCGGGT CGTGCTCATG TACCGCTTCG AGGAGGAGCT ATTCTTGCGC 2641 AGCTTGCAAG ACTATAAGAT TCAATCTGCC CTGCTGGTGC CCACACTATT TAGCTTCTTC 2701 GCTAAGAGCA CTCTCATCGA CAAGTACGAC CTAAGCAACT TGCACGAGAT CGCCAGCGGC 2761 GGGGCGCCGC TCAGCAAGGA GGTAGGTGAG GCCGTGGCCA AACGCTTCCA CCTACCAGGC 2821 ATCCGCCAGG GCTACGGCCT GACAGAAACA ACCAGCGCCA TTCTGATCAC CCCCGAAGGG 2881 GACGACAAGC CTGGCGCAGT AGGCAAGGTG GTGCCCTTCT TCGAGGCTAA GGTGGTGGAC 2941 TTGGACACCG GTAAGACACT GGGTGTGAAC CAGCGCGGCG AGCTGTGCGT CCGTGGCCCC 3001 ATGATCATGA GCGGCTACGT TAACAACCCC GAGGCTACAA ACGCTCTCAT CGACAAGGAC 3061 GGCTGGCTGC ACAGCGGCGA CATCGCCTAC TGGGACGAGG ACGAGCACTT CTTCATCGTG 3121 GACCGGCTGA AGAGCCTGAT CAAATACAAG GGCTACCAGG TAGCCCCAGC CGAACTGGAG 3181 AGCATCCTGC TGCAACACCC CAACATCTTC GACGCCGGGG TCGCCGGCCT GCCCGACGAC 3241 GATGCCGGCG AGCTGCCCGC CGCAGTCGTC GTGCTGGAAC ACGGTAAAAC CATGACCGAG 3301 AAGGAGATCG TGGACTATGT GGCCAGCCAG GTTACAACCG CCAAGAAGCT GCGCGGTGGT 3361 GTTGTGTTCG TGGACGAGGT GCCTAAAGGA CTGACCGGCA AGTTGGACGC CCGCAAGATC 3421 CGCGAGATTC TCATTAAGGC CAAGAAGGGC GGCAAGATCG CCGTGTAATA ATTCTAGAGT 3481 CGGGGCGGCC GGCCGCTTCG AGCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC 3541 ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA 3601 TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG 3661 TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT 3721 GGTAAAATCG ATAAGGATCC GTCGACCGAT GCCCTTGAGA GCCTTCAACC CAGTCAGCTC 3781 CTTCCGGTGG GCGCGGGGCA TGACTATCGT CGCCGCACTT ATGACTGTCT TCTTTATCAT 3841 GCAACTCGTA GGACAGGTGC CGGCAGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC 3901 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT 3961 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA 4021 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC 4081 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC 4141 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG 4201 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA 4261 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG 4321 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC 4381 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG 4441 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT 4501 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT 4561 CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC 4621 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT 4681 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT 4741 AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT 4801 GGTCTGACAG CGGCCGCAAA TGCTAAACCA CTGCAGTGGT TACCAGTGCT TGATCAGTGA 4861 GGCACCGATC TCAGCGATCT GCCTATTTCG TTCGTCCATA GTGGCCTGAC TCCCCGTCGT 4921 GTAGATCACT ACGATTCGTG AGGGCTTACC ATCAGGCCCC AGCGCAGCAA TGATGCCGCG 4981 AGAGCCGCGT TCACCGGCCC CCGATTTGTC AGCAATGAAC CAGCCAGCAG GGAGGGCCGA 5041 GCGAAGAAGT GGTCCTGCTA CTTTGTCCGC CTCCATCCAG TCTATGAGCT GCTGTCGTGA 5101 TGCTAGAGTA AGAAGTTCGC CAGTGAGTAG TTTCCGAAGA GTTGTGGCCA TTGCTACTGG 5161 CATCGTGGTA TCACGCTCGT CGTTCGGTAT GGCTTCGTTC AACTCTGGTT CCCAGCGGTC 5221 AAGCCGGGTC ACATGATCAC CCATATTATG AAGAAATGCA GTCAGCTCCT TAGGGCCTCC 5281 GATCGTTGTC AGAAGTAAGT TGGCCGCGGT GTTGTCGCTC ATGGTAATGG CAGCACTACA 5341 CAATTCTCTT ACCGTCATGC CATCCGTAAG ATGCTTTTCC GTGACCGGCG AGTACTCAAC 5401 CAAGTCGTTT TGTGAGTAGT GTATACGGCG ACCAAGCTGC TCTTGCCCGG CGTCTATACG 5461 GGACAACACC GCGCCACATA GCAGTACTTT GAAAGTGCTC ATCATCGGGA ATCGTTCTTC 5521 GGGGCGGAAA GACTCAAGGA TCTTGCCGCT ATTGAGATCC AGTTCGATAT AGCCCACTCT 5581 TGCACCCAGT TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCGGGGT GTGCAAAAAC 5641 AGGCAAGCAA AATGCCGCAA AGAAGGGAAT GAGTGCGACA CGAAAATGTT GGATGCTCAT 5701 ACTCGTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TACTAGTACG TCTCTCAAGG 5761 ATAAGTAAGT AATATTAAGG TACGGGAGGT ATTGGACAGG CCGCAATAAA ATATCTTTAT 5821 TTTCATTACA TCTGTGTGTT GGTTTTTTGT GTGAATCGAT AGTACTAACA TACGCTCTCC 5881 ATCAAAACAA AACGAAACAA AACAAACTAG CAAAATAGGC TGTCCCCAGT GCAAGTGCAG 5941 GTGCCAGAAC ATTTCTCT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???