pTSBX-CMV-Kars1-3×FLAG-EF1a-CopGFP-Puro plasmid

PVT107227

pTSBX-CMV-Kars1-3×FLAG-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pTSBX-CMV-Kars1(mouse)-3×FLAG-EF1a-CopGFP-Puro
Alias: NM_001130868.2;AA589550; AL024334; AL033315; AL033367;D8Ertd698e; D8Wsu108e; Kars1; LysRS; mKIAA0070
Gene lenght: 1872
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pTSBX-CMV-Kars1-3×FLAG-EF1a-CopGFP-Puro plasmid Description

pTSBX-CMV-Kars1-3×FLAG-EF1a-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pTSBX-CMV-Kars1-3×FLAG-EF1a-CopGFP-Puro plasmid Sequence

1 TGGAAGGGCT AATTCACTCC CAAAGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA 61 CACAAGGCTA CTTCCCTGAT TAGCAGAACT ACACACCAGG GCCAGGGGTC AGATATCCAC 121 TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCC AGATAAGGTA GAAGAGGCCA 181 ATAAAGGAGA GAACACCAGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG 241 AGAGAGAAGT GTTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC GTGGCCCGAG 301 AGCTGCATCC GGAGTACTTC AAGAACTGCT GATATCGAGC TTGCTACAAG GGACTTTCCG 361 CTGGGGACTT TCCAGGGAGG CGTGGCCTGG GCGGGACTGG GGAGTGGCGA GCCCTCAGAT 421 CCTGCATATA AGCAGCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA 481 GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT 541 TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC 601 AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG 661 CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG 721 CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA 781 AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG 841 AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG 901 CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT 961 GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT 1021 CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA 1081 CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC 1141 AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG 1201 TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC 1261 AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG 1321 GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC 1381 CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC 1441 GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT 1501 GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA 1561 ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA 1621 GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT 1681 AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT 1741 GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA 1801 TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT 1861 ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT 1921 CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA 1981 CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTCGACGG TATCGCCTTT AAAAGAAAAG 2041 GGGGGATTGG GGGGTACAGT GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC 2101 AAACTAAAGA ATTACAAAAA CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG 2161 ACAGCAGAGA TCCAGTTTAT CGATAAGCTT GGGAGTTCCG CGTTACATAA CTTACGGTAA 2221 ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG 2281 TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT 2341 AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG 2401 TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC 2461 CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC 2521 AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA 2581 TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA 2641 ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 2701 GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC 2761 TCCATAGAAG ACACCGACTC TACTAGAGGA TCTATTTCCG GTGAATTCGC CACCatgttg 2821 atgcaagctg ctgtcaggct tgttaggggg gccctgcgcc aaacctcatg ggcagaatgg 2881 ggtcagaggg aacttcgact gggccacctt gctcctttca caacgctcca caaggaccag 2941 ccactttctg atagaagaag tgagctgaag aggcgtctga aagctgagaa gaaactggca 3001 gagaaggagg ccaagcagaa agagctgagt gagaaacagc taaaccagac tgcttccgct 3061 cccaaccaca cggctgacaa tggcgtgggt gctgaggagg agactctgga cccaaatcaa 3121 tactacaaga tccgaagtca agccgtccag cagctgaagg tcactgggga ggatccgtac 3181 ccacacaagt tccacgtgga catctcactc actcagttca tccaagaata tagtcacctg 3241 cagcctgggg accacctgac tgatgtcacc ctcaaagtgg caggccgcat ccacgccaag 3301 agggcctctg gagggaagct catcttctat gacctgcgag gagagggggt caagttacaa 3361 gtcatggcca actccaggaa ttacaaatca gaggaggaat ttgttcatat caataacaaa 3421 ctgcgccggg gagacataat tggagttgag ggcaatcccg ggaaaaccaa gaagggcgag 3481 ctgagcatca tcccccagga gatcacgctg ctgtccccct gcttgcacat gctgcctcat 3541 cttcactttg gcctcaaaga caaggaaaca cggtatcgtc aaagatactt ggacttgatc 3601 ctgaacgact ttgtgaggca gaaatttatc gtccgctcta agatcatcac atacataaga 3661 agtttcttgg atgagctggg cttcctagag atcgaaactc ccatgatgaa catcattccc 3721 gggggagctg tggccaagcc tttcatcacc tatcacaatg agctggacat gaacttgtat 3781 atgagaattg ctccagaact ctaccacaag atgctggtgg ttggtggcat tgaccgggtt 3841 tatgaaattg ggcgccagtt ccggaacgaa gggattgatt tgactcacaa tcctgagttc 3901 accacctgtg agttctacat ggcctatgca gactatcatg acctcatgga gatcacagag 3961 aagatgctgt cagggatggt gaagagcatt acaggcagtt acaagatcac ctaccaccca 4021 gatgggccgg aaggccaagc ctacgaggtc gacttcaccc cacccttccg aagaatcagc 4081 atggtagaag agcttgagaa agccctaggt gtgaagctgc cagaaaccag tctctttgaa 4141 actgaagaaa ctcggaaaat tcttgatgat atttgtgttg caaaagctgt tgaatgcccc 4201 ccacctcgga ccacagccag gctccttgat aagctcgttg gcgagttcct cgaagtcaca 4261 tgcatcagcc ctaccttcat ctgtgatcac ccacagatca tgagtccttt ggccaaatgg 4321 caccgctcca aagagggtct cacggagcgc tttgagctgt ttgtcatgaa gaaggagata 4381 tgcaatgcct atactgagct gaatgacccc gtgcggcaga ggcagctgtt tgaggagcag 4441 gccaaggcca aggctgccgg tgatgacgag gccatgttca tagacgagaa cttctgtact 4501 gccctggaat acgggctgcc tcccacagcg ggctggggca tgggcattga tcggctcacc 4561 atgtttctca ccgattccaa caatatcaag gaagtacttc tgtttcctgc catgaagcca 4621 gaggacaaga aggaaactgc agcgaccact gaaaccccag agagcacaga ggccagcccc 4681 tctgtcGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT 4741 GACGATGACA AGTAGCTCGA GGCGATCGCT CCGGTGCCCG TCAGTGGGCA GAGCGCACAT 4801 CGCCCACAGT CCCCGAGAAG TTGGGGGGAG GGGTCGGCAA TTGAACGGGT GCCTAGAGAA 4861 GGTGGCGCGG GGTAAACTGG GAAAGTGATG TCGTGTACTG GCTCCGCCTT TTTCCCGAGG 4921 GTGGGGGAGA ACCGTATATA AGTGCAGTAG TCGCCGTGAA CGTTCTTTTT CGCAACGGGT 4981 TTGCCGCCAG AACACAGCTG AAGCTTCGAG GGGCTCGCAT CTCTCCTTCA CGCGCCCGCC 5041 GCCCTACCTG AGGCCGCCAT CCACGCCGGT TGAGTCGCGT TCTGCCGCCT CCCGCCTGTG 5101 GTGCCTCCTG AACTGCGTCC GCCGTCTAGG TAAGTTTAAA GCTCAGGTCG AGACCGGGCC 5161 TTTGTCCGGC GCTCCCTTGG AGCCTACCTA GACTCAGCCG GCTCTCCACG CTTTGCCTGA 5221 CCCTGCTTGC TCAACTCTAC GTCTTTGTTT CGTTTTCTGT TCTGCGCCGT TACAGATCCA 5281 AGCTGTGACC GGCGCCTACG CTAGACGCCA CCATGGAGAG CGACGAGAGC GGCCTGCCCG 5341 CCATGGAGAT CGAGTGCCGC ATCACCGGCA CCCTGAACGG CGTGGAGTTC GAGCTGGTGG 5401 GCGGCGGAGA GGGCACCCCC AAGCAGGGCC GCATGACCAA CAAGATGAAG AGCACCAAAG 5461 GCGCCCTGAC CTTCAGCCCC TACCTGCTGA GCCACGTGAT GGGCTACGGC TTCTACCACT 5521 TCGGCACCTA CCCCAGCGGC TACGAGAACC CCTTCCTGCA CGCCATCAAC AACGGCGGCT 5581 ACACCAACAC CCGCATCGAG AAGTACGAGG ACGGCGGCGT GCTGCACGTG AGCTTCAGCT 5641 ACCGCTACGA GGCCGGCCGC GTGATCGGCG ACTTCAAGGT GGTGGGCACC GGCTTCCCCG 5701 AGGACAGCGT GATCTTCACC GACAAGATCA TCCGCAGCAA CGCCACCGTG GAGCACCTGC 5761 ACCCCATGGG CGATAACGTG CTGGTGGGCA GCTTCGCCCG CACCTTCAGC CTGCGCGACG 5821 GCGGCTACTA CAGCTTCGTG GTGGACAGCC ACATGCACTT CAAGAGCGCC ATCCACCCCA 5881 GCATCCTGCA GAACGGGGGC CCCATGTTCG CCTTCCGCCG CGTGGAGGAG CTGCACAGCA 5941 ACACCGAGCT GGGCATCGTG GAGTACCAGC ACGCCTTCAA GACCCCCATC GCCTTCGCCA 6001 GATCCCGCGC TCAGTCGTCC AATTCTGCCG TGGACGGCAC CGCCGGACCC GGCTCCACCG 6061 GATCTCGCGA GGGCAGAGGA AGTCTTCTAA CATGCGGTGA CGTGGAGGAG AATCCCGGCC 6121 CTATGACCGA GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG 6181 TACGCACCCT CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG 6241 ACCGCCACAT CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG 6301 ACATCGGCAA GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG 6361 AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG 6421 GTTCCCGGCT GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG 6481 AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG 6541 GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC 6601 TGGAGACCTC CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG 6661 CCGACGTCGA GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT 6721 GAACGCGTCT GGAACAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT 6781 CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT 6841 GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT 6901 CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT 6961 GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC 7021 GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG 7081 ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA GCTGACGTCC 7141 TTTCCATGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC 7201 GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG 7261 CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC 7321 CCGCCTGGAA TTAATTCTGC AGTCGAGACC TAGAAAAACA TGGAGCAATC ACAAGTAGCA 7381 ATACAGCAGC TACCAATGCT GATTGTGCCT GGCTAGAAGC ACAAGAGGAG GAGGAGGTGG 7441 GTTTTCCAGT CACACCTCAG GTACCTTTAA GACCAATGAC TTACAAGGCA GCTGTAGATC 7501 TTAGCCACTT TTTAAAAGAA AAGAGGGGAC TGGAAGGGCT AATTCACTCC CAACGAAGAC 7561 AAGATATCCT TGATCTGTGG ATCTACCACA CACAAGGCTA CTTCCCTGAT TAGCAGAACT 7621 ACACACCAGG GCCAGGGGTC AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC 7681 CAGTTGAGCC AGATAAGGTA GAAGAGGCCA ATAAAGGAGA GAACACCAGC TTGTTACACC 7741 CTGTGAGCCT GCATGGGATG GATGACCCGG AGAGAGAAGT GTTAGAGTGG AGGTTTGACA 7801 GCCGCCTAGC ATTTCATCAC GTGGCCCGAG AGCTGCATCC GGAGTACTTC AAGAACTGCT 7861 GATATCGAGC TTGCTACAAG GGACTTTCCG CTGGGGACTT TCCAGGGAGG CGTGGCCTGG 7921 GCGGGACTGG GGAGTGGCGA GCCCTCAGAT CCTGCATATA AGCAGCTGCT TTTTGCCTGT 7981 ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC 8041 CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG 8101 TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT 8161 AGCAGTAGTA GTTCATGTCA TCTTATTATT CAGTATTTAT AACTTGCAAA GAAATGAATA 8221 TCAGAGAGTG AGAGGCCTTG ACATTGCTAG CGTTTACCGT CGACCTCTAG CTAGAGCTTG 8281 GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC 8341 AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC 8401 ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG 8461 CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT 8521 TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC 8581 TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA 8641 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT 8701 AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC 8761 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT 8821 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG 8881 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG 8941 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT 9001 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG 9061 ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC 9121 GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA 9181 AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT 9241 GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT 9301 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA 9361 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC 9421 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT 9481 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA 9541 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA 9601 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA 9661 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA 9721 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG 9781 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA 9841 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT 9901 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT 9961 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA 10021 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT 10081 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA 10141 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC 10201 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG 10261 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC 10321 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 10381 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 10441 CCTGACGTCG ACGGATCGGG AGATCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA 10501 AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG 10561 TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGATC AACTGGATAA CTCAAGCTAA 10621 CCAAAATCAT CCCAAACTTC CCACCCCATA CCCTATTACC ACTGCCAATT ACCTGTGGTT 10681 TCATTTACTC TAAACCTGTG ATTCCTCTGA ATTATTTTCA TTTTAAAGAA ATTGTATTTG 10741 TTAAATATGT ACTACAAACT TAGTAGTTTT TAAAGAAATT GTATTTGTTA AATATGTACT 10801 ACAAACTTAG TAGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.