pTSBX-CMV-Ppp6r3-3×FLAG-EF1a-CopGFP-Puro plasmid

PVT109344

pTSBX-CMV-Ppp6r3-3×FLAG-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pTSBX-CMV-Ppp6r3(mouse)-3×FLAG-EF1a-CopGFP-Puro
Alias: NM_001360429.1;4930528G08Rik; 9130026N02Rik; D19Bwg1430e;D19Ertd703e; mKIAA1558; Pp6r3; Ppcs3; Pptcs3; Sapl; Saps3
Gene lenght: 2637
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pTSBX-CMV-Ppp6r3-3×FLAG-EF1a-CopGFP-Puro plasmid Description

pTSBX-CMV-Ppp6r3-3×FLAG-EF1a-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pTSBX-CMV-Ppp6r3-3×FLAG-EF1a-CopGFP-Puro plasmid Sequence

1 TGGAAGGGCT AATTCACTCC CAAAGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA 61 CACAAGGCTA CTTCCCTGAT TAGCAGAACT ACACACCAGG GCCAGGGGTC AGATATCCAC 121 TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCC AGATAAGGTA GAAGAGGCCA 181 ATAAAGGAGA GAACACCAGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG 241 AGAGAGAAGT GTTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC GTGGCCCGAG 301 AGCTGCATCC GGAGTACTTC AAGAACTGCT GATATCGAGC TTGCTACAAG GGACTTTCCG 361 CTGGGGACTT TCCAGGGAGG CGTGGCCTGG GCGGGACTGG GGAGTGGCGA GCCCTCAGAT 421 CCTGCATATA AGCAGCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA 481 GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT 541 TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC 601 AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG 661 CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG 721 CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA 781 AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG 841 AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG 901 CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT 961 GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT 1021 CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA 1081 CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC 1141 AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG 1201 TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC 1261 AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG 1321 GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC 1381 CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC 1441 GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT 1501 GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA 1561 ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA 1621 GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT 1681 AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT 1741 GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA 1801 TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT 1861 ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT 1921 CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA 1981 CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTCGACGG TATCGCCTTT AAAAGAAAAG 2041 GGGGGATTGG GGGGTACAGT GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC 2101 AAACTAAAGA ATTACAAAAA CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG 2161 ACAGCAGAGA TCCAGTTTAT CGATAAGCTT GGGAGTTCCG CGTTACATAA CTTACGGTAA 2221 ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG 2281 TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT 2341 AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG 2401 TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC 2461 CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC 2521 AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA 2581 TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA 2641 ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 2701 GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC 2761 TCCATAGAAG ACACCGACTC TACTAGAGGA TCTATTTCCG GTGAATTCGC CACCatgttt 2821 tggaaatttg atcttcactc atcatcccac atagacacgc ttctagagag agaagatgtg 2881 acactgaagg agctaatgga tgaggaggat gttctacagg agtgtaaagc acagaaccgc 2941 aagctcatag aatttctgtt aaaagcagaa tgtctggaag atttagtctc attcattata 3001 gaagaaccac ctcaagatat ggatgagaag atccgataca agtatccaaa tatatcttgt 3061 gagttgctca catctgatgt ctcccagatg aatgatagac tgggggaaga tgagtccttg 3121 ctaatgaaat tgtatagctt cctcctaaat gagtccccct tgaacccact actcgccagt 3181 ttcttcagca aggtgctaag tattcttatc agcagaaaac cagaacagat tgtggatttc 3241 ttgaaaaaga agcgtgattt tgtggatctc attataaagc acataggaac ttctgctatc 3301 atggatttat tgctcaggct cttgacatgt attgagcctc cacaacccag gcaagatgtt 3361 ctgaattggc taaatgagga gaggattatc cagaggcttg tggaaatagt tcacccatcg 3421 caagaagaag atcggcattc aaatgcctca caatcacttt gtgaaattgt tcgcctgagc 3481 agagaccaga tgttacaagt tcagaacagt acagagccag accccctgct tgccactcta 3541 gaaaagcaag aaattattga gcagcttctg tcaaatattt ttcacaagga gaagaatgaa 3601 tcggccatag tcagtgcaat tcagattttg ctgactttgc ttgagactcg acggccaacg 3661 tttgaaggcc atatagaaat ctgccccccg ggcatgagtc attcagcttg ttcagttaac 3721 aagagtgttc ttgaagccat ccgaggaaga cttggatctt tccatgaact cctactcgag 3781 ccacccaaga aaagtgtgat gaagactaca tggggtatat tggacccacc tgtggggaat 3841 actcgattaa atgtcattag gttgatatcc agcctgctcc aaaccaatac cagcagcata 3901 aatggagacc tgatggaact gaacagcatt ggcgtcatat tggatatgtt cttcaagtac 3961 acatggaata actttctaca cacgcaagtg gaaatttgca ttgctctcat tctggccagt 4021 ccttttgaga atgcagagaa tggcacaatt actgatcaag actccactgg tgacaatttg 4081 ttattgaagc atctttttca aaagtgtcag ttaatagagc gaatacttga agcctgggac 4141 acaaatgaga agaaacaggc tgaaggagga cgacggcatg gttacatggg acaccttacg 4201 aggatagcta actgtattgt gcacagcact gacaagggtc cgaacagtgc gttagtacag 4261 cagctcatca aagatcttcc cgatgaagtc agggagcggt gggagacgtt ctgcacgaac 4321 tccttaggag agacgaacaa gaggaacacg gtagatctag ttacaacctg ccatattcat 4381 tcatccagtg atgatgaaat tgactttaaa gacacgggtt tctcacagga ttcctcttta 4441 cagcaagcct tttctgatta tcagatgcaa caaatgacgt ccaattttat tgaccagttt 4501 ggcttcaacg atgagaagtt tgcagaccaa gacgacattg gcaatgtttc ttttgatcgg 4561 gtgtcagata tcaactttac tctcaataca aatgaaagtg gaaatattgc cctgtttgaa 4621 gcatgttgta aagaacggat acaacaattt gatgatggcg gctctgatga agaggatatc 4681 tgggaggaga agcacattgc atttactccc gaatcccaga ggaggtccag ctctggcagt 4741 acggacagtg aagaaagtac agactctgaa gaagaagatg gggccaaaca agacctgttt 4801 gagtccagca gtgcaaacac agaggacaaa atggaggtgg atctgaatga gccacccacc 4861 tggtcagcca actttgacgt cccaatggaa accacccatg gtgctccctt agactccgtg 4921 ggatccgatg tctggagcac agaggagcca atgccaacta aagagacagg ctgggcttcc 4981 ttttcagagt tcacatcctc cctgagcaca aaggagtctt taaggagtaa ttcgcctgtg 5041 gaaatggaaa ccagcactga atccgtggac cccttgacac ctggtgcagc tgccttggcc 5101 actcagccag aggcaccagg cagtatggcc atggaagcca gctcagatgg ggaagaggat 5161 gctgagagta cagacaaggt aactgagacc gtgatgaatg gcggcatgaa ggaaacactc 5221 agcctcactg tagacgccaa gacagagact gcagtcttca aaagagtgtt gaagtcctac 5281 cgtgaggaag aaaaactgtc tacctctcaa gatgctgctt gcaaagatgc agaagagacc 5341 ccagagccag cagaggccaa gtgcacagct cccctgactc ccagcagtag tcccgagcaa 5401 aggaccgacc agccaagcat gccaagcgac ccatcagtta atggccctgt aGGATCCGAC 5461 TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC 5521 AAGTAGCTCG AGGCGATCGC TCCGGTGCCC GTCAGTGGGC AGAGCGCACA TCGCCCACAG 5581 TCCCCGAGAA GTTGGGGGGA GGGGTCGGCA ATTGAACGGG TGCCTAGAGA AGGTGGCGCG 5641 GGGTAAACTG GGAAAGTGAT GTCGTGTACT GGCTCCGCCT TTTTCCCGAG GGTGGGGGAG 5701 AACCGTATAT AAGTGCAGTA GTCGCCGTGA ACGTTCTTTT TCGCAACGGG TTTGCCGCCA 5761 GAACACAGCT GAAGCTTCGA GGGGCTCGCA TCTCTCCTTC ACGCGCCCGC CGCCCTACCT 5821 GAGGCCGCCA TCCACGCCGG TTGAGTCGCG TTCTGCCGCC TCCCGCCTGT GGTGCCTCCT 5881 GAACTGCGTC CGCCGTCTAG GTAAGTTTAA AGCTCAGGTC GAGACCGGGC CTTTGTCCGG 5941 CGCTCCCTTG GAGCCTACCT AGACTCAGCC GGCTCTCCAC GCTTTGCCTG ACCCTGCTTG 6001 CTCAACTCTA CGTCTTTGTT TCGTTTTCTG TTCTGCGCCG TTACAGATCC AAGCTGTGAC 6061 CGGCGCCTAC GCTAGACGCC ACCATGGAGA GCGACGAGAG CGGCCTGCCC GCCATGGAGA 6121 TCGAGTGCCG CATCACCGGC ACCCTGAACG GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG 6181 AGGGCACCCC CAAGCAGGGC CGCATGACCA ACAAGATGAA GAGCACCAAA GGCGCCCTGA 6241 CCTTCAGCCC CTACCTGCTG AGCCACGTGA TGGGCTACGG CTTCTACCAC TTCGGCACCT 6301 ACCCCAGCGG CTACGAGAAC CCCTTCCTGC ACGCCATCAA CAACGGCGGC TACACCAACA 6361 CCCGCATCGA GAAGTACGAG GACGGCGGCG TGCTGCACGT GAGCTTCAGC TACCGCTACG 6421 AGGCCGGCCG CGTGATCGGC GACTTCAAGG TGGTGGGCAC CGGCTTCCCC GAGGACAGCG 6481 TGATCTTCAC CGACAAGATC ATCCGCAGCA ACGCCACCGT GGAGCACCTG CACCCCATGG 6541 GCGATAACGT GCTGGTGGGC AGCTTCGCCC GCACCTTCAG CCTGCGCGAC GGCGGCTACT 6601 ACAGCTTCGT GGTGGACAGC CACATGCACT TCAAGAGCGC CATCCACCCC AGCATCCTGC 6661 AGAACGGGGG CCCCATGTTC GCCTTCCGCC GCGTGGAGGA GCTGCACAGC AACACCGAGC 6721 TGGGCATCGT GGAGTACCAG CACGCCTTCA AGACCCCCAT CGCCTTCGCC AGATCCCGCG 6781 CTCAGTCGTC CAATTCTGCC GTGGACGGCA CCGCCGGACC CGGCTCCACC GGATCTCGCG 6841 AGGGCAGAGG AAGTCTTCTA ACATGCGGTG ACGTGGAGGA GAATCCCGGC CCTATGACCG 6901 AGTACAAGCC CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC 6961 TCGCCGCCGC GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA 7021 TCGAGCGGGT CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA 7081 AGGTGTGGGT CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG 7141 AAGCGGGGGC GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC 7201 TGGCCGCGCA GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT 7261 GGTTCCTGGC CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG 7321 TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT 7381 CCGCGCCCCG CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG 7441 AGGTGCCCGA AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGAACGCGTC 7501 TGGAACAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT 7561 GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT 7621 TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG 7681 GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC 7741 CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC 7801 CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT 7861 CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AGCTGACGTC CTTTCCATGG 7921 CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG 7981 GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG 8041 CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCCTGGA 8101 ATTAATTCTG CAGTCGAGAC CTAGAAAAAC ATGGAGCAAT CACAAGTAGC AATACAGCAG 8161 CTACCAATGC TGATTGTGCC TGGCTAGAAG CACAAGAGGA GGAGGAGGTG GGTTTTCCAG 8221 TCACACCTCA GGTACCTTTA AGACCAATGA CTTACAAGGC AGCTGTAGAT CTTAGCCACT 8281 TTTTAAAAGA AAAGAGGGGA CTGGAAGGGC TAATTCACTC CCAACGAAGA CAAGATATCC 8341 TTGATCTGTG GATCTACCAC ACACAAGGCT ACTTCCCTGA TTAGCAGAAC TACACACCAG 8401 GGCCAGGGGT CAGATATCCA CTGACCTTTG GATGGTGCTA CAAGCTAGTA CCAGTTGAGC 8461 CAGATAAGGT AGAAGAGGCC AATAAAGGAG AGAACACCAG CTTGTTACAC CCTGTGAGCC 8521 TGCATGGGAT GGATGACCCG GAGAGAGAAG TGTTAGAGTG GAGGTTTGAC AGCCGCCTAG 8581 CATTTCATCA CGTGGCCCGA GAGCTGCATC CGGAGTACTT CAAGAACTGC TGATATCGAG 8641 CTTGCTACAA GGGACTTTCC GCTGGGGACT TTCCAGGGAG GCGTGGCCTG GGCGGGACTG 8701 GGGAGTGGCG AGCCCTCAGA TCCTGCATAT AAGCAGCTGC TTTTTGCCTG TACTGGGTCT 8761 CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT 8821 AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC 8881 TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGTAGT 8941 AGTTCATGTC ATCTTATTAT TCAGTATTTA TAACTTGCAA AGAAATGAAT ATCAGAGAGT 9001 GAGAGGCCTT GACATTGCTA GCGTTTACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA 9061 TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA 9121 GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT 9181 GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA 9241 ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC 9301 ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG 9361 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC 9421 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC 9481 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA 9541 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC 9601 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT 9661 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG 9721 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC 9781 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA 9841 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT 9901 AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT 9961 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG 10021 CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG 10081 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA 10141 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA 10201 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG 10261 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA 10321 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG 10381 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT 10441 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT 10501 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC 10561 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA 10621 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT 10681 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC 10741 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA 10801 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA 10861 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA 10921 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT 10981 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC 11041 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA 11101 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 11161 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC 11221 GACGGATCGG GAGATCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG 11281 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA 11341 ACTCATCAAT GTATCTTATC ATGTCTGGAT CAACTGGATA ACTCAAGCTA ACCAAAATCA 11401 TCCCAAACTT CCCACCCCAT ACCCTATTAC CACTGCCAAT TACCTGTGGT TTCATTTACT 11461 CTAAACCTGT GATTCCTCTG AATTATTTTC ATTTTAAAGA AATTGTATTT GTTAAATATG 11521 TACTACAAAC TTAGTAGTTT TTAAAGAAAT TGTATTTGTT AAATATGTAC TACAAACTTA 11581 GTAGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.