pTYB1
PVTY01190 2ug
pTYB1 Description
Plasmid type: E.coli Expression Vector Size: 7477 bp 5' sequencing primers: T7 Tags: Intein Resistance(s): Ampicillin (Amp) Note: This C-terminal fusion vector is designed for the in-frame insertion of a target gene into a polylinker upstream of an intein tag (the Sce VMA intein/chitin binding domain, 55 kDa). This results in the fusion of the C-terminus of the target protein to the N-terminus of the intein tag. Thiol-induced self-cleavage of the intein releases the target protein from the chitin-bound intein tag, resulting in a single column purification of the target protein. |
Caution:
1. This product is FOR RESEARCH USE ONLY!