pUC19
PVT0003 2ug
pUC19 Information
Promoter: Lac/lac promoter
Replicon: ColE1 ori
Plasmid classification: Escherichia coli vector; cloned plasmid
Plasmid size: 2686bp
Plasmid label: LacZ
Prokaryotic resistance: ampicillin Amp
Cloned strain: DH5 alpha
Culture conditions: 37 centigrade, aerobic, LB
5'sequencing primers: M13F:TGTAAAACGACGGCCAGT
3'sequencing primers: M13R:CAGGAAACAGCTATGACC
Note: selection of blue leukoplakia
pUC19 Description
The size of pUC18 and pUC19 is only 2686bp, the most commonly used plasmid vector with compact structure, almost free of excess DNA, and GenBank registration number of L08752 (pUC18) and X02514 (pUC19). From pBR322, lacZ (MSC) comes from M13mp18/19. The structure of these two plasmids is almost the same, but the direction of the polyclonal sites is opposite. These plasmids lack ROP gene that controls copy number, so the copy number is 500-700. The pUC series contains a partial coding sequence of the amino terminal of a lacZ protein, which can exhibit alpha complementarity in specific receptor cells. Therefore, after inserting foreign fragments into polyclonal sites, the recombinant plasmids can be screened through blue and white colonies formed by alpha complementarity. PUC18 and pUC19 vectors are suitable for cloning DNA fragments, sequencing DNA, and expressing foreign genes. Because of the polyclonal loci in the lacZ field, it can be easily screened by blue and white to judge whether there is a DNA fragment insertion in the carrier when the lacZ deficient cell is transformed into a host (such as JM109 and so on) and is cultured on a flat medium containing IPTG and X-Gal. At the same time, foreign genes can be expressed by lac promoter on the vector, and DNA fragments in insertion vectors can be sequenced. DNA sequencing can facilitate the use of M13 universal primers.
pUC19 Multiple site
pUC19 Sequence
LOCUS Exported File 2686 bp ds-DNA circular SYN 02-2-2015
KEYWORDS pUC19
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2686)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported 2015-2-2
FEATURES Location/Qualifiers
source 1..2686
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 106..127
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 142..172
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 180..196
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 204..220
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
CDS 216..539
/codon_start=1
/gene="lacZ?fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/note="lacZ-alpha"
/translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
DAA"
misc_feature 233..289
/gene="
"
/note="MCS"
/note="pUC18/19 multiple cloning site"
primer_bind complement(290..306)
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 780..884
/gene="bla"
/note="AmpR promoter"
CDS 885..1745
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 1916..2504
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
481 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
541 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
601 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
661 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
721 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
781 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
841 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
901 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
961 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
1021 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
1081 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
1141 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
1201 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
1261 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
1321 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
1381 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
1441 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
1501 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
1561 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
1621 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
1681 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
1741 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
1801 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
1861 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
1921 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
1981 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
2041 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
2101 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
2161 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
2221 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
2281 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
2341 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
2401 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
2461 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
2521 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
2581 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
2641 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaaga
//
Caution:
- This product is FOR RESEARCH USE ONLY!
- The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
- Shipping temperature is 2-8℃.
Search name
pUC19,Plasmid pUC19,pUC19 vector
English