pUC57-Fap plasmid

PVT101019

pUC57-Fap plasmid Information

Plasmid name: pUC57-Fap(mouse)
Alias: NM_007986.3;SIMP;
Gene lenght: 2283bp
Host: E.coli
Use(s): Gene Template
Fragment type: cDNA
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter:
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: lac-P
Reverse primer:
Copy number: High
Induction:

pUC57-Fap plasmid Description

pUC57-Fap is a plasmid suitable for gene templates in Escherichia coli.

pUC57-Fap plasmid Sequence

1 GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 61 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 121 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 181 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 241 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 301 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 361 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 421 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 481 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 541 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 601 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 661 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 721 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 781 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 841 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 901 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 961 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 1021 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 1081 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 1141 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 1201 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 1261 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 1321 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC 1381 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 1441 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 1501 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 1561 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 1621 AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 1681 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 1741 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 1801 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 1861 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 1921 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 1981 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 2041 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 2101 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 2161 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG 2221 ACCATGATTA CGCCAAGCTT GCATGCAGGC CTCTGCAGTC GACGGGCCCG GGATCCatga 2281 agacatggct gaaaactgtc tttggagtta ccaccctggc tgcgcttgct ttagtggtga 2341 tatgcattgt cttacgtccc tcaagagttt acaaacctga aggaaacaca aagagagctc 2401 ttaccttgaa ggatatttta aatggaacat tctcatataa aacatatttt cccaactgga 2461 tttcagaaca agaatatctt catcaatctg aggatgataa catagtattt tataatattg 2521 aaacaagaga atcatatatc attttgagta atagcaccat gaaaagtgtg aatgctacag 2581 attatggttt gtcacctgat cggcaatttg tgtatctaga aagtgattat tcaaagctct 2641 ggcgatattc atacacagcg acatactaca tctacgacct tcagaatggg gaatttgtaa 2701 gaggatacga gctccctcgt ccaattcagt atctatgctg gtcgcctgtt gggagtaaat 2761 tagcatatgt atatcaaaac aatatttatt tgaaacaaag accaggagat ccaccttttc 2821 aaataactta tactggaaga gaaaatagaa tatttaatgg aataccagac tgggtttatg 2881 aagaggaaat gcttgccaca aaatatgctc tttggtggtc tccagatgga aaatttttgg 2941 catatgtaga atttaatgat tcagatatac caattattgc ctattcttat tatggtgatg 3001 gacagtatcc tagaactata aatattccat atccaaaggc tggggctaag aatccggttg 3061 ttcgtgtttt tattgttgac accacctacc ctcaccacgt gggcccaatg gaagtgccag 3121 ttccagaaat gatagcctca agtgactatt atttcagctg gctcacatgg gtgtccagtg 3181 aacgagtatg cttgcagtgg ctaaaaagag tgcagaatgt ctcagtcctg tctatatgtg 3241 atttcaggga agactggcat gcatgggaat gtccaaagaa ccaggagcat gtagaagaaa 3301 gcagaacagg atgggctggt ggattctttg tttcgacacc agcttttagc caggatgcca 3361 cttcttacta caaaatattt agcgacaagg atggttacaa acatattcac tacatcaaag 3421 acactgtgga aaatgctatt caaattacaa gtggcaagtg ggaggccata tatatattcc 3481 gcgtaacaca ggattcactg ttttattcta gcaatgaatt tgaaggttac cctggaagaa 3541 gaaacatcta cagaattagc attggaaact ctcctccgag caagaagtgt gttacttgcc 3601 atctaaggaa agaaaggtgc caatattaca cagcaagttt cagctacaaa gccaagtact 3661 atgcactcgt ctgctatggc cctggcctcc ccatttccac cctccatgat ggccgcacag 3721 accaagaaat acaagtatta gaagaaaaca aagaactgga aaattctctg agaaatatcc 3781 agctgcctaa agtggagatt aagaagctca aagacggggg actgactttc tggtacaaga 3841 tgattctgcc tcctcagttt gacagatcaa agaagtaccc tttgctaatt caagtgtatg 3901 gtggtccttg cagccagagt gttaagtctg tgtttgctgt taattggata acttatctcg 3961 caagtaagga ggggatagtc attgccctgg tagatggtcg gggcactgct ttccaaggtg 4021 acaaattcct gcatgccgtg tatcgaaaac tgggtgtata tgaagttgag gaccagctca 4081 cagctgtcag aaaattcata gaaatgggtt tcattgatga agaaagaata gccatatggg 4141 gctggtccta cggaggttat gtttcatccc tggcccttgc atctggaact ggtcttttca 4201 aatgtggcat agcagtggct ccagtctcca gctgggaata ttacgcatct atctactcag 4261 agagattcat gggcctccca acaaaggacg acaatctcga acactataaa aattcaactg 4321 tgatggcaag agcagaatat ttcagaaatg tagactatct tctcatccac ggaacagcag 4381 atgataatgt gcactttcag aactcagcac agattgctaa agctttggtt aatgcacaag 4441 tggatttcca ggcgatgtgg tactctgacc agaaccatgg tatatcatct gggcgctccc 4501 agaatcattt atatacccac atgacgcact tcctcaagca atgcttttct ttatcagacT 4561 AGAATTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC 4621 TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA 4681 CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGCGCCTG ATGCGGTATT 4741 TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATG GTGCACTCTC AGTACAATCT 4801 GCTCTGATGC CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT 4861 GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT 4921 GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???