Plasmid classification: plant series plasmid; plant report plasmid; dual fluorescent plasmid
PUC-SPYNE plasmid is a double fluorescent plasmid.
LOCUS Exported 4356 bp ds-DNA circular SYN 08-NOV-2017
DEFINITION synthetic circular DNA
KEYWORDS pUC-SPYNE
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4356)
FEATURES Location/Qualifiers
source 1..4356
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(1..9)
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 483..587
/gene="bla"
/note="AmpR promoter"
CDS 588..1448
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 1619..2207
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 2495..2516
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2531..2561
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 2569..2585
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2593..2609
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
promoter 3133..3478
/note="CaMV 35S promoter"
/note="strong constitutive promoter from cauliflower mosaic
virus"
misc_feature 3487..3570
/note="MCS"
CDS 3574..3603
/codon_start=1
/product="Myc (human c-Myc oncogene) epitope tag"
/note="Myc"
/translation="EQKLISEEDL"
CDS 3604..4071
/codon_start=1
/note="YFP-N"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
terminator 4088..4340
/note="NOS terminator"
/note="nopaline synthase terminator and poly(A) signal"
primer_bind complement(4349..4356)
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
61 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
121 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc
181 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
241 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
301 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
361 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
421 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
481 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
541 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
601 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
661 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
721 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
781 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
841 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
901 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
961 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
1021 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
1081 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
1141 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
1201 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
1261 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
1321 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
1381 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
1441 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
1501 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
1561 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
1621 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
1681 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
1741 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
1801 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
1861 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
1921 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
1981 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
2041 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
2101 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
2161 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
2221 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
2281 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
2341 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
2401 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
2461 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
2521 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
2581 aacaatttca cacaggaaac agctatgacc atgattacgc caagcttgca tgcctgcagg
2641 tccccagatt agccttttca atttcagaaa gaatgctaac ccacagatgg ttagagaggc
2701 ttacgcagca ggtctcatca agacgatcta cccgagcaat aatctccagg aaatcaaata
2761 ccttcccaag aaggttaaag atgcagtcaa aagattcagg actaactgca tcaagaacac
2821 agagaaagat atatttctca agatcagaag tactattcca gtatggacga ttcaaggctt
2881 gcttcacaaa ccaaggcaag taatagagat tggagtctct aaaaaggtag ttcccactga
2941 atcaaaggcc atggagtcaa agattcaaat agaggaccta acagaactcg ccgtaaagac
3001 tggcgaacag ttcatacaga gtctcttacg actcaatgac aagaagaaaa tcttcgtcaa
3061 catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag tctcagaaga
3121 ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca
3181 ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa
3241 atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc
3301 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc
3361 ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca
3421 ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg agagaacacg
3481 ggggactcta gagttaaccg ggctcaggcc tggcgcgcca ctagtggatc catcgatagt
3541 actgtcgacc tcgagggtac cgctcccggg atggagcaaa agttgatttc tgaggaggat
3601 cttatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
3661 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
3721 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
3781 accctcgtga ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg
3841 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
3901 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
3961 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
4021 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccta agagctcgaa
4081 tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg
4141 tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat
4201 gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat
4261 ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt
4321 gtcatctatg ttactagatc gggaattcac tggccg
//
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