pUMVC
Search name
pUMVC,Plasmid pUMVC,pUMVC vector
pUMVC Information
Promoter: CMV
Replicon: pUC ori
Terminator: beta -globin poly (A) signal
Plasmid classification: virus series, lentivirus cloning vector
Plasmid size: 9287bp
Prokaryotic resistance: Kan
Cloned strain: Stbl3
Culture conditions: 37 centigrade, aerobic LB
Expression host: mammalian cells
Induction mode: no induction, instantaneous expression
5'sequencing primers: CMV-F:CGCAAATGGGCGGTAGGCGTG
3'sequencing primers: primers designed according to sequence
Use:Lentiviral
pUMVC Description
PUMVC is a low copy plasmid, cultured at 37. It is a slow virus clone plasmid. The plasmid was transformed into Stbl3.
pUMVC Sequence
LOCUS Exported 9287 bp ds-DNA circular SYN 30-AUG-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 5
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9287)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Tuesday, August 30, 2016 from SnapGene Viewer 3.1.4
FEATURES Location/Qualifiers
source 1..9287
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin complement(117..705)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1246..2061)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
enhancer 2686..3065
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 3066..3269
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
intron 3405..3674
/note="CMV intron"
/note="modified intron A from human cytomegalovirus"
CDS 3728..4144
/note="gag (truncated)"
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
misc_feature 8504..8878
/note="pol region"
/note="Moloney murine leukemia virus (MMLV) pol region
containing the splice acceptor site"
polyA_site 9085..9140
/note="beta-globin poly(A) signal"
/note="rabbit beta-globin polyadenylation signal"
ORIGIN
1 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
61 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
121 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
181 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
241 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
301 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
361 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
421 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
481 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
541 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
601 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
661 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
721 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
781 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
841 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
901 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactcgg gggggggggc
961 gctgaggtct gcctcgtgaa gaaggtgttg ctgactcata ccaggcctga atcgccccat
1021 catccagcca gaaagtgagg gagccacggt tgatgagagc tttgttgtag gtggaccagt
1081 tggtgatttt gaacttttgc tttgccacgg aacggtctgc gttgtcggga agatgcgtga
1141 tctgatcctt caactcagca aaagttcgat ttattcaaca aagccgccgt cccgtcaagt
1201 cagcgtaatg ctctgccagt gttacaacca attaaccaat tctgattaga aaaactcatc
1261 gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa
1321 aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc
1381 ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc
1441 gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa
1501 tggcaaaagc ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc
1561 atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg
1621 aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag
1681 gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg
1741 gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat
1801 aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc
1861 atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc
1921 gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca
1981 tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctcg agcaagacgt
2041 ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag cagacagttt
2101 tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac
2161 aacgtggctt tccccccccc ccattattga agcatttatc agggttattg tctcatgagc
2221 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
2281 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
2341 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
2401 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
2461 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
2521 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
2581 aggagaaaat accgcatcag attggctatt ggccattgca tacgttgtat ccatatcata
2641 atatgtacat ttatattggc tcatgtccaa cattaccgcc atgttgacat tgattattga
2701 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
2761 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
2821 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
2881 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
2941 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
3001 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
3061 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
English