pUTKM1

PVT6021    2ug

pUTKM1 Information

Prokaryotic resistance: Ampicillin

Screening Marker: Kanamycin

Cloning strain: s17-1 Escherichia coli

Culture conditions: 37 degrees

Plasmid host: Escherichia coli

Plasmid use: gene knockout

pUTKM1 Sequence

gccccgtggccgggggactgttgggcgccatctccttgctgcctcgcgcgtttcggtgatgacggtgaaaacctctgaca
catgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgg
gtgttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcggagtgtatactggcttaactatgcggcat
cagagcagattgtactgagagtgcaccataaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgc
ttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataac
tacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttat
cagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaat
tgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctgcaggcatcgtggt
gtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgt
gcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatg
gcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcatt
ctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaacacgggataataccgcgccacatagcagaactt
taaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatg
taacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggca
aaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagca
tttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcaca
tttccccgaaaagtgccacctgcagatctgcaggtcgaccgatcagatcttgatcccctgcgccatcagatccttggcgg
caagaaagccatccagtttactttgcagggcttcccaaccttcccagagggcgccccagctggcaattccggttcgcttg
ctgtccataaaaccgcccagtctagctatcgccatgtaagcccactgcaagctacctgctttctctttgcgcttgcgttt
tcccttgtccagatagcccagtagctgacattcatccggggtcagcaccgtttctgcggactggctttctacgtgttccg
cttcctttagcagcccttgcgccctgagtgcttgcggcagcgtgaagctttctctgagctgtaacagcctgaccgcaaca
aacgagaggatcgagaccatccgctccagattatccggctcctccatgcgttgcctctcggctcctgctccggttttcca
tgccttatggaactcctcgatccgccagcgatgggtataaatgtcgatgacgcgcaaggcttgggctagcgactcgaccg
gttcgctggtcagcaacaaccatttcaacggggtctcacccttgggcgggttaatctcctcggccagcaccgcgttgagc
gtgatattcccctgttttagcgtgatgcgcccactgcgcaggctcaagctcgccttgcgggctggtcgatttttacgttt
accgcgtttatccaccacgcccttttgcggaatgctgatctgatagccacccaactccggttggttcttcagatggtcgt
acagatacaacccagactctacgtccttgcgtgggtgcttggagcgcaccacgaagcgctcgttatgcgccagtttgtcc
tgcagataagcatgaatatcggcttcgcggtcacagaccgcaatcacgttgctcatcatgctgcccatgcgtaaccggct
agttgcggcggctgccagccatttgccactctccttttcatccgcatcggcagggtcatccgggcgcatccaccactcct
gatgcagtaatcctacggtgcggaatgtggtggcctcgagcaagagaacggagtgaacccaccatccgcgggatttatcc
tgaatagagcccagcttgccaagctcttcggcgacctggtggcgataactcaaagaggtggtgtcctcaatggccagcag
ttcgggaaactcctgagccaacttgactgtttgcatggcgccagcctttctgatcgcctcggcagaaacgttgggattgc
ggataaatcggtaagcgccttcctgcatggcttcactaccctctgatgagatggttattgatttaccagaatattttgcc
aattgggcggcgacgttaaccaagcgggcagtacggcgaggatcacccagcgccgccgaagagaacacagatttagccca
gtcggccgcacgatgaagagcagaagttatcatgaacgttaccatgttaggaggtcacatggaagatcagatcctggaaa
acgggaaaggttccgttcaggacgctacttgtgtacgggatcggtcgacggatcccaagcttcttctagactgtctcttg
atcagatctggccgcctaggccgaattcgagctcggtacccggggatccggctgtaatccgggcagcgcaacggaacatt
catcagtgtaaaaatggaatcaataaagccctgcgcagcgcgcagggtcagcctgaatacgcgtttaatgaccagcacag
tcgtgatggcaaggtcagaatagcgctgaggtctgcctcgtgaagaaggtgttgctgactcataccaggcctgaatcgcc
ccatcatccagccagaaagtgagggagccacggttgatgagagctttgttgtaggtggaccagttggtgattttgaactt
ttgctttgccacggaacggtctgcgttgtcgggaagatgcgtgatctgatccttcaactcagcaaaagttcgatttattc
aacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactg
tctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcgaggccgcgattaaa
ttccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgat
tgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatg
gtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggtt
actcaccactgcgatccccgggaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatg
cgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctc
gctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttga
acaagtctggaaagaaatgcataagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgata
accttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatctt
gccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcc
tgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagaattggttaattggttgtaacactggc
agagcattacgctgacttgacgggacggcggctttgttgaataaatcgaacttttgctgagttgaaggatcagatcacgc
atcttcccgacaacgcagaccgttccgtggcaaagcaaaagttcaaaatcaccaactggtccacctacaacaaagctctc
atcaaccgtggctccctcactttctggctggatgatggggcgattcaggcctggtatgagtcagcaacaccttcttcacg
aggcagacctcagcgctattctgaccttgccatcacgactgtgctggtcattaaacgcgtattcaggctgaccctgcgcg
ctgcgcagggctttattgattccatttttacactgatgaatgttccgttgcgctgcccggattacagccggatcctctag
agtcgacctgcaggcatgcaagcttcggccgcctaggccgcggccgcacttgtgtataagagtcagaattccacaaattg
ttatccgctcacaattccacatgtggaattccacatgtggaattcccatgtcagccgttaagtgttcctgtgtcactcaa
aattgctttgagaggctctaagggcttctcagtgcgttacatccctggcttgttgtccacaaccgttaaaccttaaaagc
tttaaaagccttatatattcttttttttcttataaaacttaaaaccttagaggctatttaagttgctgatttatattaat
tttattgttcaaacatgagagcttagtacgtgaaacatgagagcttagtacgttagccatgagagcttagtacgttagcc
atgagggtttagttcgttaaacatgagagcttagtacgttaaacatgagagcttagtacgtgaaacatgagagcttagta
cgtactatcaacaggttgaactgctgatcttcagatcctctacgccggacgcatcgtggccggatccagccgaccaggct
ttccacgcccgcgtgccgctccatgtcgttcgcgcggttctcggaaacgcgctgccgcgtttcgtgattgtcacgctcaa
gcccgtagtcccgttcgagcgtcgcgcagaggtcagcgagggcgcggtaggcccgatacggctcatggatggtgtttcgg
gtcgggtgaatcttgttgatggcgatatggatgtgcaggttgtcggtgtcgtgatgcacggcactgacgcgctgatgctc
ggcgaagccaagcccagcgcagatgcggtcctcaatcgcgcgcaacgtctccgcgtcgggcttctctcccgcgcggaagc
taaccagcaggtgataggtcttgtcggcctcggaacgggtgttgccgtgctgggtcgccatcacctcggccatgacagcg
ggcagggtgtttgcctcgcagttcgtgacgcgcacgtgacccaggcgctcggtcttgccttgctcgtcggtgatgtactt
caccagctccgcgaagtcgctcttcttgatggagcgcatggggacgtgcttggcaatcacgcgcaccccccggccgtttt
agcggctaaaaaagtcatggctctgccctcgggcggaccacgcccatcatgaccttgccaagctcgtcctgcttctcttc
gatcttcgccagcagggcgaggatcgtggcatcaccgaaccgcgccgtgcgcgggtcgtcggtgagccagagtttcagca
ggccgcccaggcggcccaggtcgccattgatgcgggccagctcgcggacgtgctcatagtccacgacgcccgtgattttg
tagccctggccgacggccagcaggtaggccgacaggctcatgccggccgccgccgccttttcctcaatcgctcttcgttc
gtctggaaggcagtacaccttgataggtgggctgcccttcctggttggcttggtttcatcagccatccgcttgccctcat
ctgttacgccggcggtagccggccagcctcgcagagcaggattcccgttgagcaccgccaggtgcgaataagggacagtg
aagaaggaacacccgctcgcgggtgggcctacttcacctatcctgcccggctgacgccgttggatacaccaaggaaagtc
tacacgaaccctttggcaaaatcctgtatatcgtgcgaaaaaggatggatataccgaaaaaatcgctataatgaccccga
agcagggttatgcagcggaaaagcgctgcttccctgctgttttgtggaatatctaccgactggaaacaggcaaatgcagg
aaattactgaactgaggggacaggcgagagacgatgccaaagagctacaccgacgagctggccgagtgggttgaatcccg
cgcggccaagaagcgccggcgtgatgaggctgcggttgcgttcctggcggtgagggcggatgtcgaggcggcgttagcgt
ccggctatgcgctcgtcaccatttgggagcacatgcgggaaacggggaaggtcaagttctcctacgagacgttccgctcg
cacgccaggcggcacatcaaggccaagcccgccgatgtgcccgcaccgcaggccaaggctgcggaacccgcgccggcacc
caagacgccggagccacggcggccgaagcaggggggcaaggctgaaaagccggcccccgctgcggccccgaccggcttca
ccttcaacccaacaccggacaaaaaggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggt
tgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcg
tgggtatggtggcag
//

Caution:
1.  This product is FOR RESEARCH USE ONLY!
2.  The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.  Shipping temperature is 2-8℃.

Search name

pUTKM1,Plasmid pUTKM1,pUTKM1 vector, pUTKM1 sequence, pUTKM1 map, pUTKM1 cloning