PVRL2 plasmid

PVT91471

PVRL2 plasmid Information

Plasmid name: PVRL2
Alias: --
Gene lenght:
Host: Acinetobacter
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Gen
Screening marker:
Fluorescent labeling:
Promoter: araBAD
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: PBAD30.F
Reverse primer:
Copy number: High
Induction: Arabinose Induction

PVRL2 plasmid Description

PVRL2 is a plasmid suitable for protein expression in Acinetobacter.

PVRL2 plasmid Sequence

1 tcgaggtcga cggtatcgat aagcttgata tcgaattcct gcagcccggg ggatccacta 61 gttctagagc ggccgccacc gcggtggagc tccaattcgc cctatagtga gtcgtattac 121 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa 181 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc 241 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggaaatt gtaaccatgg 301 tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac 361 gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat 421 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 481 gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa 541 cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag 601 aaggccatcc tgacggatgg cctttttgcg tttccatggt cgtgatacgc ctatttttat 661 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg 721 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 781 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 841 cgaattgaca taagcctgtt cggttcgtaa actgtaatgc aagtagcgta tgcgctcacg 901 caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat 961 ggcttgttat gactgttttt ttgtacagtc tatgcctcgg gcatccaagc agcaagcgcg 1021 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagcaacg 1081 atgttacgca gcagggcagt cgccctaaaa caaagttagg tggctcaagt atgggcatca 1141 ttcgcacatg taggctcggc cctgaccaag tcaaatccat gcgggctgct cttgatcttt 1201 tcggtcgtga gttcggagac gtagccacct actcccaaca tcagccggac tccgattacc 1261 tcgggaactt gctccgtagt aagacattca tcgcgcttgc tgccttcgac caagaagcgg 1321 ttgttggcgc tctcgcggct tacgttctgc ccaagtttga gcagccgcgt agtgagatct 1381 atatctatga tctcgcagtc tccggcgagc accggaggca gggcattgcc accgcgctca 1441 tcaatctcct caagcatgag gccaacgcgc ttggtgctta tgtgatctac gtgcaagcag 1501 attacggtga cgatcccgca gtggctctct atacaaagtt gggcatacgg gaagaagtga 1561 tgcactttga tatcgaccca agtaccgcca cctaacaatt cgttcaagcc gagatcggct 1621 tcccggccgc ggagttgttc ggtaaattgt cacaacgccg cggccaattc gcatcgattg 1681 tcagaccaag tatgcagccc ctgctgctgt tgctgctgtt caagtgtcaa ttgctctact 1741 ctgttgctcg tctcaatgat agacatatca agcctttcag cttttttatt gcgctttttg 1801 atttcatcat tacgcttgac gatctccagt tcctcccccc tgcgcttggc gtgggtggct 1861 tcaagtccct ccttgaatgt cggctcgatg tctagtccac gatccttatg actgcggtga 1921 tcaactctca cctcaagccc tgctcgctct aggtggacgt tcgtcagatc cgccactttt 1981 tccctgattt ttttcagcgt tgagttctga tctagctccc tgactttctt ccctagccct 2041 tgcggtgtaa ggcttcttgt tgtcattagt atgtgggcgt gatgggtttc ttttcatcac 2101 ttcccccatg cacatgaggg gcatggatcg ctacgtccac cgccactcca taggctttaa 2161 ctaaggactg gcacaactcg gttaacaagt tgcctgacgc ctgtgtctta tgtgggcgtg 2221 aggtttcttt ctccttcccc catgcacatg aggggcatgg atcgctacgt ccaccgccac 2281 tccataggct ttaactaagg actggcacaa ctcggtaaca agtgcctgac gctgtgtctt 2341 atccagttca tgcggcaatg ctatctctac ttcctttgct aatcttgcct cctgtttgat 2401 gtcaccgttc ttttttagtt cggattgctc tacccgattc catagggttt gacgatctaa 2461 catgtcagga cttgccccag ttggggcaaa aatttgggtg tattcaatgc ctgttttttt 2521 ggtgtagtcc tgctcttttc cgtacgtatc acagtacaat ttttcgcctg ctcggtatgc 2581 tgcacatgcc acgattgagc gaccatctga cctcgaaatg ttctgcattt cacaatggta 2641 aattgccatt ttcatcacct gtttattaac agccgcccca agttttgggc ggttggggtg 2701 tcggggtttc cctgaccgta cttgcaaaat ttggcgtagc aaaaattttg cgtaagtgcg 2761 cccttcggga actctgcgag gctaaaagca aaagcaagag caataaggct ttgactttgt 2821 tttttgattt tgctcgctgc gctcggatct tgatgtaagt cgagtttttg aagtagaaca 2881 cttttcacat tgatgatggt tcatacttcg gaaaataggt tgagacacag gcattaaaaa 2941 tggtcaaaaa aacagctatg gaattgggtc aaatgctcga tgaagaaaag gaaaaattgg 3001 aacgtcagga aaaggcaaga aaggatctag ctgacgctgt ggtgaaaggt cgagagcaaa 3061 aacaggcaag gtcagacgat gcaaaacgca agatcctgat cggggcttac ttactgaaga 3121 aacatgataa tgacatcaag aaacttgtgg aacaaaaccc cgattttatt gggtacatta 3181 gagaaaatga taagcactta ttttcatagg cattagtagt tataaaaaac cctcataaat 3241 cgagggtttc ttttttatct gcattacttt ttgaatcaat gtactttttt agcttgctat 3301 tttttcactg catacagccg cttatactct ttctcaatca atgcggtcaa aatggctttt 3361 tggctctccc ctgctgcgcc caccatgtcc gcaagcatat ccgacacacc cttatctata 3421 aacacctcca aacgttgact atcaaggctt ttgcgttttt cacgatatgc tttttgtcgt 3481 tcggcattgg tttttggcgg ctcttttagc atgtcctgtg ttttctgatc gttcgggtct 3541 ttcatgtctt taactcccat atagtatatc atagttacag gataacattg tttttagtta 3601 caggataact ttttataaaa tttatttgta aaaatttatg ctatcctgca actattttta 3661 aattacagga taactattta acgttaccct gtaactatag ttatcctgta acgctaaatt 3721 aattaccctg taacatatat tttgttatcc tgtaacaact aaatattgaa ataatactac 3781 caaagttacc ctgtaacgaa aataactagg ttaaaaaatg atcggatttt aacattttgc 3841 gttgttccaa aagttatcaa cagcctagaa cgtcatagga agcgattaca gacactttat 3901 agctatcagc atgggaacat aagggcagga tgaaatatgg gtcttaaaac gcaaatggtg 3961 aggttttaga ggtatttttt gaagatgatt aaggcggttt gtttttaaaa tttttggcgg 4021 ctctcaggct gcttacattt taaccagttc agtgaaaagt tctttttcag caaatttctg 4081 tttagcacca tagctaaaac ttgcgtggaa catattaaga attgaccgaa atgacacaat 4141 ctcaattata ttttttttga aaagttttct ttatcaaata ttttaaatca ttgatttata 4201 tataagtata cattcatttt aataattaat ctttatttaa caatgattta tctatattca 4261 attgtttaat tattcttact aatattatct ctatatcaat attttttatt taaaaacata 4321 tgtttagtag tgcttttgat taaagtacca gagggaggga gcagagctga atgggaaata 4381 ctcaccctag agcgattctt aaaaatcacc ctaaagtatt cccattcgat gtaccgtcgg 4441 tcggtcgctt tcgcatcagg gatgacatca ctgtatcaag ctgccactgt tatgattacg 4501 attgatagca ccgcctgaac acgctcataa ccgccaatta aatgactact cattgcgtcc 4561 gctactctgt tcagttcccc tagtaatagc gtttttccga tgtgtgctag cgtcactgta 4621 cctcatcacc cacacatgga cagattgagt tacagacatt ggctaaattt ttggggtcta 4681 tgcttgacaa agcgagttaa aaccttagaa tttaagacag gtacattaag cccctgtggt 4741 gaaatcatta gcggtcgtca aaccttaatg ctttcgattg ccgatatgtc agtatcgaag 4801 atctgtaccc cataaattac ggtaaagccc caagcaattg caaggggctt ttatcttttt 4861 taacaaaaaa aatttataaa tcaggatttt ataacactaa ataatccaaa gtacatgagt 4921 aagtcatgac cactcctgcg atgtgtgtag cactctgagt atccgtatta tatcagtgat 4981 gtcatataca accacataat gcggatgtat cactaactcc ctagtatctt tctgtctgcc 5041 tgtgcgcccc atcagtcgat tatcaacaag taaatctgtc ttttcttcaa ttaaatcatc 5101 aatttcaata gctgctatag ggttgcgttc ttcaagataa tcatagatat ttctacgatc 5161 ttggcgagct gtttcagtcc actcaatctt cattcttcgc tgccagtttc gccaatgttg 5221 cagcacgtct agcggcaaac tctgccttta cgtcctcatg tggaatcaac ttgcctgcat 5281 tggcttcatt taagccgatc tgtacctgct gcttgaacca tttgtcatag tctgcggctt 5341 cctgttgctt cttaacaaag tcacgcatga agtcacggat tagctgcgcc cccgaccgat 5401 caacagtctt agcaaggctt gaaaattctt gctttaggtc atggtctacc cgaaaggtaa 5461 aagtcgcttc tgtcatgttt ttcactccaa tgtgttacat ttgaatgcta ttgtaacaca 5521 ttaaaaagtt aggtcattgt cttttttcgg cttaggctgt tcttggtcat aacgtggttt 5581 aaatgatggc tgtcgctcat tctcaagtaa aattttccgt ttattaacag cctgttgcat 5641 ctgctcatag taaggcatca actgcggtaa aatatcagca agtgcctgtc tgtctttatc 5701 caggatacgg ctcaattgcc cgattttttt caagtcacgc tcataggcta ttctgtcgct 5761 gatctgctcg tatttttgct ttgaacacct tatgtactct tgcatatcgt tacttgccca 5821 tgcctgcgcc tgaagacgtg taaacatgga aatgtctgaa gcaacatggt cgctgatatg 5881 gtcatactca gcatctttga catagccgta ttttgataaa tatctttcat agtcctgccg 5941 tttctttttg tcatcttcct gtttttgttt agtttctctt tctatctgct gttgttttcg 6001 ttgctgttcc tgttgctcct cttttatctc tctgtgataa atggaaaggc tagattgatc 6061 attatcccaa cgtgtgagca tgtcctcaaa gccgtaataa tcagcactaa accaacctgt 6121 tttcactttc actggtggcg gcagtggctc tccgatctcc cttagcagtg ctgtagctgc 6181 tcgtccctct ttgatcactt tatcaagctg caagcggtcg atattgtctt gcaaagcctt 6241 gtaaaatcta tgctctgctc gcttctcatc cgtttttact tcaatcaaat acacatctat 6301 aagcgtttgg cggtgttgtt ggtggtcgta tagtgcctgt tgtgcggtgg catagactgc 6361 gggggcttgg tctacggcat cctccagggg ggatcacaag tttactcata tatactttag 6421 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 6481 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 6541 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 6601 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 6661 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg 6721 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 6781 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 6841 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 6901 agcttggagc gaacgaccta caccgaacag agatacctac agcgtgagct atgagaaagc 6961 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 7021 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 7081 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 7141 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 7201 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag 7261 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa 7321 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 7381 agctggcacg acaggtttcc cgactggaaa gatgcataat gtgcctgtca aatggacgaa 7441 gcagggattc tgcaaaccct atgctactcc gtcaagccgt caattgtctg attcgttacc 7501 aattatgaca acttgacggc tacatcattc actttttctt cacaaccggc acggaactcg 7561 ctcgggctgg ccccggtgca ttttttaaat acccgcgaga aatagagttg atcgtcaaaa 7621 ccaacattgc gaccgacggt ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc 7681 tggctgatac gttggtcctc gcgccagctt aagacgctaa tccctaactg ctggcggaaa 7741 agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa 7801 ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg attatccatc 7861 ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga 7921 tttatcgcca gcagctccga atagcgccct tccccttgcc cggcgttaat gatttgccca 7981 aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc gaaagaaccc cgtattggca 8041 aatattgacg gccagttaag ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac 8101 tggtgatacc attcgcgagc ctccggatga cgaccgtagt gatgaatctc tcctggcggg 8161 aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc cctgattttt caccaccccc 8221 tgaccgcgaa tggtgagatt gagaatataa cctttcattc ccagcggtcg gtcgataaaa 8281 aaatcgagat aaccgttggc ctcaatcggc gttaaacccg ccaccagatg ggcattaaac 8341 gagtatcccg gcagcagggg atcattttgc gcttcagcca tacttttcat actcccgcca 8401 ttcagagaag aaaccaattg tccatattgc atcagacatt gccgtcactg cgtcttttac 8461 tggctcttct cgctaaccaa accggtaacc ccgcttatta aaagcattct gtaacaaagc 8521 gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc 8581 cacattgatt atttgcacgg cgtcacactt tgctatgcca tagcattttt atccataaga 8641 ttagcggatc ctacctgacg ctttttatcg caactctcta ctgtttctcc atacccgttt 8701 ttttgggcta accttctgaa tc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???