pXT7-Cas9
pXT7-Cas9 Information
Alternative name: pXT7
Plasmid host: Wide host
Prokaryotic resistance: Amp
Promoter: T7
Duplicator: pBR322
Perceived state: Stbl3
Temperature: 37 degrees
Forward primer: T7:
Note: Induction
pXT7-Cas9 Description
PXT7-Cas9 is a Xenopus gene editing Cas9 expression plasmid.
pXT7-Cas9 Sequence
TAGCGTATCAGAGTCTCGTCCGTCGGTCGTATGACGGTGAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTT
GTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAAC
TATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATA
CCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCC
AGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACG
ACGGCCAGTGAATTGGATTTAGGTGACACTATAGAATACGAATTAATTCGAGCTCGCCCGGGGATCCTCTAGAGTCGACC
TGCAGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGAATGTGAAGAAACTTTCTTTTTA
TTAGGAGCAGATACGAATGGCTACATTTTGGGGGACAACATTTTGTAAAGTGTAAGTTGGTATTATGTAGCTTAGAGACT
CCATTCGGGTGTTCTTGAGGCTGGTTTAGTGGTAACCAGATCCTAGTCAGTCACTAGATTATTTCTTTTTCTTAGCTTGA
CCAGCTTTCTTAGTAGCAGCAGGACGCTTGTCTCCACCGAGCTGAGAGAGGTCGATTCTTGTTTCATAGAGCCCCGTAAT
TGACTGATGAATCAGTGTGGCGTCCAGGACCTCCTTTGTAGAGGTGTACCGCTTTCTGTCTATGGTGGTGTCGAAGTACT
TGAAGGCTGCAGGCGCGCCCAAGTTGGTCAGAGTAAACAAGTGGATAATGTTTTCTGCCTGCTCCCTGATGGGCTTATCC
CTGTGCTTATTGTAAGCAGAAAGCACCTTATCGAGGTTAGCGTCGGCGAGGATCACTCTTTTGGAGAATTCGCTTATTTG
CTCGATGATCTCATCAAGGTAGTGTTTGTGTTGTTCCACGAACAGCTGCTTCTGCTCATTATCTTCGGGAGACCCTTTGA
GCTTTTCATAGTGGCTGGCCAGATACAAGAAATTAACGTATTTAGAGGGCAGTGCCAGCTCGTTACCTTTCTGCAGCTCG
CCCGCACTAGCGAGCATTCGTTTCCGGCCGTTTTCAAGCTCAAAGAGAGAGTACTTGGGAAGCTTAATGATGAGGTCTTT
TTTGACCTCTTTATATCCTTTCGCCTCGAGAAAGTCGATGGGGTTTTTTTCGAAGCTTGATCGCTCCATGATTGTGATGC
CCAGCAGTTCCTTGACGCTTTTGAGTTTTTTAGACTTCCCTTTCTCCACTTTGGCCACAACCAGTACACTGTAAGCGACT
GTAGGAGAATCGAATCCGCCGTATTTCTTGGGGTCCCAATCTTTTTTGCGTGCGATCAGCTTGTCGCTGTTCCTTTTCGG
GAGGATACTTTCCTTGGAGAAGCCTCCGGTCTGTACTTCGGTCTTTTTAACGATGTTCACCTGCGGCATGGACAGGACCT
TCCGGACTGTCGCGAAATCCCTACCCTTGTCCCACACGATTTCTCCTGTTTCTCCGTTTGTTTCGATAAGTGGTCGCTTC
CGAATCTCTCCATTGGCCAGTGTAATCTCGGTCTTGAAAAAATTCATAATATTGCTGTAAAAGAAGTACTTAGCGGTGGC
CTTGCCTATTTCCTGCTCAGACTTTGCGATCATTTTCCTAACATCGTACACTTTATAGTCTCCGTAAACAAATTCAGATT
CAAGCTTGGGATATTTTTTGATAAGTGCAGTGCCTACCACTGCATTCAGGTAGGCATCATGCGCATGGTGGTAATTGTTG
ATCTCTCTCACCTTATAAAACTGAAAGTCCTTTCTGAAATCTGAGACCAGCTTAGACTTCAGAGTAATAACTTTCACCTC
TCGAATCAGTTTGTCATTTTCATCGTACTTGGTGTTCATGCGTGAATCGAGAATTTGGGCCACGTGCTTGGTGATCTGGC
GTGTCTCAACAAGCTGCCTTTTGATGAAGCCTGCTTTATCCAACTCAGACAGGCCACCTCGTTCAGCCTTAGTCAGATTA
TCGAACTTCCGTTGTGTGATCAGTTTGGCGTTCAGCAGCTGCCGCCAATAATTTTTCATTTTCTTGACAACTTCTTCTGA
GGGGACGTTATCACTCTTCCCTCTATTTTTATCGGATCTTGTCAACACTTTATTATCAATAGAATCATCTTTGAGAAAAG
ACTGGGGCACGATATGATCCACGTCGTAGTCGGAGAGCCGATTGATGTCCAGTTCCTGATCCACGTACATGTCCCTGCCG
TTCTGCAGGTAGTACAGGTAGAGCTTCTCATTCTGAAGCTGGGTGTTTTCAACTGGGTGTTCCTTAAGGATTTGGGACCC
CAGTTCTTTTATACCCTCTTCAATCCTCTTCATCCTTTCCCTACTGTTCTTCTGTCCCTTCTGGGTAGTTTGGTTCTCTC
GGGCCATCTCGATAACGATATTCTCGGGCTTATGCCTTCCCATTACTTTGACGAGTTCATCCACGACCTTAACGGTCTGC
AGTATTCCCTTTTTGATAGCTGGGCTACCTGCAAGATTAGCGATGTGCTCGTGAAGACTGTCCCCCTGGCCAGAAACTTG
TGCTTTCTGGATGTCCTCCTTAAAGGTGAGAGAGTCATCATGGATCAACTGCATGAAGTTCCGGTTGGCAAATCCATCGG
ACTTAAGAAAATCCAGGATTGTCTTTCCACTCTGCTTGTCTCGGATCCCATTGATCAGTTTTCTTGACAGCCGCCCCCAT
CCTGTATATCGGCGCCTCTTGAGCTGTTTCATGACTTTGTCGTCGAAGAGATGAGCGTAAGTTTTCAAGCGTTCTTCAAT
CATCTCCCTATCTTCAAACAACGTAAGGGTGAGGACAATGTCCTCAAGAATGTCCTCGTTCTCCTCATTGTCCAGGAAGT
CCTTGTCTTTAATGATTTTCAGGAGATCGTGATACGTTCCCAGGGATGCGTTGAAGCGATCCTCCACTCCGCTGATTTCA
ACAGAGTCGAAACATTCAATCTTTTTGAAATAGTCTTCTTTGAGCTGTTTCACGGTAACTTTCCGGTTCGTCTTGAAGAG
GAGGTCCACGATAGCTTTCTTCTGCTCTCCAGACAGGAATGCTGGCTTTCTCATCCCTTCTGTGACGTATTTGACCTTGG
TGAGCTCGTTATAAACTGTGAAGTACTCGTACAGCAGAGAGTGTTTAGGAAGCACCTTTTCGTTAGGCAGATTTTTATCA
AAGTTAGTCATCCTTTCGATGAAGGACTGGGCAGAGGCCCCCTTATCCACGACTTCCTCGAAGTTCCAGGGAGTGATGGT
CTCTTCTGATTTGCGAGTCATCCACGCGAATCTGGAATTTCCCCGGGCGAGGGGGCCTACATAGTAGGGTATCCGAAATG
TGAGGATTTTCTCAATCTTTTCCCTGTTATCTTTCAAAAAGGGGTAGAAATCCTCTTGCCGCCTGAGTATAGCGTGCAGT
TCGCCCAGGTGAATCTGGTGGGGGATGCTTCCATTGTCGAAAGTGCGCTGTTTGCGCAACAGATCTTCTCTGTTAAGCTT
TACCAGCAGCTCCTCGGTGCCGTCCATTTTTTCCAAGATGGGCTTAATAAATTTGTAAAATTCCTCCTGGCTTGCTCCGC
CGTCAATGTATCCGGCGTAGCCATTTTTAGACTGATCGAAGAAAATTTCCTTGTACTTCTCAGGCAGTTGCTGTCTGACA
AGGGCCTTCAGCAAAGTCAAGTCTTGGTGGTGCTCATCATAGCGCTTGATCATACTAGCGCTCAGCGGAGCTTTGGTGAT
CTCCGTGTTCACTCGCAGAATATCACTCAGCAGAATGGCGTCTGACAGGTTCTTTGCCGCCAAAAAAAGGTCTGCGTACT
GGTCGCCGATCTGGGCCAGCAGATTGTCGAGATCATCATCGTAGGTGTCTTTGCTCAGTTGAAGCTTGGCATCTTCGGCC
AGGTCGAAGTTAGATTTAAAGTTGGGGGTCAGCCCGAGTGACAGGGCGATAAGATTACCAAACAGGCCGTTCTTCTTCTC
CCCAGGGAGCTGTGCGATGAGGTTTTCGAGCCGCCGGGATTTGGACAGCCTAGCGCTCAGGATTGCTTTGGCGTCAACTC
CGGATGCGTTGATCGGGTTCTCTTCGAAAAGCTGATTGTAAGTCTGAACCAGTTGGATAAAGAGTTTGTCGACATCGCTG
TTGTCTGGGTTCAGGTCCCCCTCGATGAGGAAGTGTCCCCGAAATTTGATCATATGCGCCAGCGCGAGATAGATCAACCG
CAAGTCAGCCTTATCAGTACTGTCTACAAGCTTCTTCCTCAGATGATATATGGTTGGGTACTTTTCATGGTACGCCACCT
CGTCCACGATATTGCCAAAGATTGGGTGGCGCTCGTGCTTTTTATCCTCCTCCACCAAAAAGGACTCCTCCAGCCTATGG
AAGAAAGAGTCATCCACCTTAGCCATCTCATTACTAAAGATCTCCTGCAGGTAGCAGATCCGATTCTTTCTGCGGGTATA
TCTGCGCCGTGCTGTTCTTTTGAGCCGCGTGGCTTCGGCCGTCTCCCCGGAGTCGAACAGGAGGGCGCCAATGAGGTTCT
TCTTTATGCTGTGGCGATCGGTATTGCCCAGAACTTTGAATTTTTTGCTCGGCACCTTGTACTCGTCCGTAATGACGGCC
CAGCCGACGCTGTTTGTGCCGATATCGAGCCCAATGGAGTACTTCTTGTCCATGGCTGCTGGGACACCGTGGATACCGAC
CTTCCGCTTCTTCTTTGGGGCCATGGTGGCGGTACCGATCTGCCAAAGTTGAGCGTTTATTCTGAGCTTCTGCAAAAAGA
ACAAGCAAGCTTGTCTCCCTATAGTGAGTCGTATTAGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATT
GTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAA
CTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCA
ACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGC
TGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATG
TGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGA
CGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG
GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTG
GCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACC
CCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC
TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAAC
TACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTC
TTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGAT
CTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATG
AGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTA
AACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTG
CCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAC
CCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTT
ATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTG
TTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGG
CGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGC
CGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGA
CTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGAT
AATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTT
ACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTT
CTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTC
TTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAA
TAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAA
CCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAG
CTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGT
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Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3. Shipping temperature is 2-8℃.
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