pYES3/CT and pYES6/CT are S. cerevisiae expression vectors derived from the parental pYES2 vector. Vectors feature a C-terminal V5 epitope for detection with an Anti-V5 Antibody. pYES3/CT carries the TRP1 selection marker for selection in yeast. pYES6/CT carries the bsd resistance gene for selection with the antibiotic Blasticidin, a potent selection agent that can be used at very low concentrations and in any strain regardless of auxotrophic marker.
LOCUS Exported 5870 bp ds-DNA circular SYN 29-NOV-2013
DEFINITION High-copy episomal vector with a TRP1 marker for galactose-inducible
expression of C-terminally tagged proteins in S. cerevisiae.
ACCESSION .
VERSION .
KEYWORDS pYES3 CT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5870)
AUTHORS Invitrogen (Life Technologies)
TITLE Direct Submission
JOURNAL Exported Sunday, September 11, 2016 from SnapGene Viewer 3.1.4
FEATURES Location/Qualifiers
source 1..5870
/organism="synthetic DNA construct"
/lab_host="Saccharomyces cerevisiae"
/mol_type="other DNA"
promoter 2..443
/gene="S. cerevisiae GAL1"
/note="GAL1 promoter"
/note="inducible promoter, regulated by Gal4"
protein_bind 2..119
/bound_moiety="Gal4"
/note="UAS"
/note="upstream activating sequence mediating
Gal4-dependent induction"
promoter 475..493
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 501..600
/note="MCS"
/note="multiple cloning site"
CDS 607..648
/codon_start=1
/product="epitope tag from simian virus 5"
/note="V5 tag"
/translation="GKPIPNPLLGLDST"
CDS 658..675
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
terminator 709..956
/gene="S. cerevisiae CYC1"
/note="CYC1 terminator"
/note="transcription terminator for CYC1"
rep_origin complement(1204..1792)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1963..2823)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKRSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2824..2928)
/gene="bla"
/note="AmpR promoter"
promoter 3031..3132
/gene="S. cerevisiae TRP1"
/note="TRP1 promoter"
CDS 3133..3807
/codon_start=1
/gene="S. cerevisiae TRP1"
/product="phosphoribosylanthranilate isomerase, required
for tryptophan biosynthesis"
/note="TRP1"
/note="yeast auxotrophic marker"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
KK"
rep_origin 4449..5329
/note="2u ori"
/note="yeast 2u plasmid origin of replication"
rep_origin complement(5398..5853)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 acggattaga agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt
61 cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga
121 acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac
181 ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga
241 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat
301 taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc
361 ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac
421 ctctatactt taacgtcaag gagaaaaaac cccggatcgg actactagca gctgtaatac
481 gactcactat agggaatatt aagcttggta ccgagctcgg atccactagt aacggccgcc
541 agtgtgctgg aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc
601 ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtcat
661 catcaccatc accattgagt ttaaacccgc tgatcctaga gggccgcatc atgtaattag
721 ttatgtcacg cttacattca cgccctcccc ccacatccgc tctaaccgaa aaggaaggag
781 ttagacaacc tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa
841 cgttatttat atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca
901 ttatactgaa aaccttgctt gagaaggttt tgggacgctc gaaggcttta atttgcaagc
961 tgcggccctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
1021 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
1081 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
1141 gaacatgtga gcaaaaggcc agcaaaagcc caggaaccgt aaaaaggccg cgttgctggc
1201 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
1261 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
1321 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
1381 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
1441 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
1501 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
1561 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
1621 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
1681 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
1741 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
1801 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
1861 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
1921 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
1981 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
2041 cgtgtagata actacgatac gggagcgctt accatctggc cccagtgctg caatgatacc
2101 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
2161 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
2221 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
2281 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
2341 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
2401 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
2461 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
2521 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac
2581 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
2641 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
2701 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
2761 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
2821 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
2881 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
2941 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
3001 gcgtatcacg aggccctttc gtcttcaaga aattcggtcg aaaaaagaaa aggagagggc
3061 caagagggag ggcattggtg actattgagc acgtgagtat acgtgattaa gcacacaaag
3121 gcagcttgga gtatgtctgt tattaatttc acaggtagtt ctggtccatt ggtgaaagtt
3181 tgcggcttgc agagcacaga ggccgcagaa tgtgctctag attccgatgc tgacttgctg
3241 ggtattatat gtgtgcccaa tagaaagaga acaattgacc cggttattgc aaggaaaatt
3301 tcaagtcttg taaaagcata taaaaatagt tcaggcactc cgaaatactt ggttggcgtg
3361 tttcgtaatc aacctaagga ggatgttttg gctctggtca atgattacgg cattgatatc
3421 gtccaactgc acggagatga gtcgtggcaa gaataccaag agttcctcgg tttgccagtt
3481 attaaaagac tcgtatttcc aaaagactgc aacatactac tcagtgcagc ttcacagaaa
3541 cctcattcgt ttattccctt gtttgattca gaagcaggtg ggacaggtga acttttggat
3601 tggaactcga tttctgactg ggttggaagg caagagagcc ccgagagctt acattttatg
3661 ttagctggtg gactgacgcc agaaaatgtt ggtgatgcgc ttagattaaa tggcgttatt
3721 ggtgttgatg taagcggagg tgtggagaca aatggtgtaa aagactctaa caaaatagca
3781 aatttcgtca aaaatgctaa gaaataggtt attactgagt agtatttatt taagtattgt
3841 ttgtgcactt gccctagctt atcgatgata agctgtcaaa gatgagaatt aattccacgg
3901 actatagact atactagata ctccgtctac tgtacgatac acttccgctc aggtccttgt
3961 cctttaacga ggccttacca ctcttttgtt actctattga tccagctcag caaaggcagt
4021 gtgatctaag attctatctt cgcgatgtag taaaactagc tagaccgaga aagagactag
4081 aaatgcaaaa ggcacttcta caatggctgc catcattatt atccgatgtg acgctgcagc
4141 ttctcaatga tattcgaata cgctttgagg agatacagcc taatatccga caaactgttt
4201 tacagattta cgatcgtact tgttacccat cattgaattt tgaacatccg aacctgggag
4261 ttttccctga aacagatagt atatttgaac ctgtataata atatatagtc tagcgcttta
4321 cggaagacaa tgtatgtatt tcggttcctg gagaaactat tgcatctatt gcataggtaa
4381 tcttgcacgt cgcatccccg gttcattttc tgcgtttcca tcttgcactt caatagcata
4441 tctttgttaa cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc
4501 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgaaag
4561 cgctatttta ccaacgaaga atctgtgctt catttttgta aaacaaaaat gcaacgcgac
4621 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa
4681 cgcgagagcg ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc
4741 atcccgagag cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc
4801 gctctataat gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa
4861 ggctactttg gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt
4921 actgattact agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat
4981 tctataccga tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct
5041 tcattggtca gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg
5101 aaatgtttac attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt
5161 ttttgtctaa agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg
5221 caagttcaag gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata
5281 tagcaaagag atacttttga gcaatgtttg tggaagcggt attcgcaatg ggaagctcca
5341 ccccggttga taatcagaaa agccccaaaa acaggaagat tgtataagca aatatttaaa
5401 ttgtaaacgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
5461 ttaacgaata gcccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
5521 ggttgagtgt tgttccagtt tccaacaaga gtccactatt aaagaacgtg gactccaacg
5581 tcaaagggcg aaaaagggtc tatcagggcg atggcccact acgtgaacca tcaccctaat
5641 caagtttttt ggggtcgagg tgccgtaaag cagtaaatcg gaagggtaaa cggatgcccc
5701 catttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
5761 aaggagcggg ggctagggcg gtgggaagtg taggggtcac gctgggcgta accaccacac
5821 ccgccgcgct taatggggcg ctacagggcg cgtggggatg atccactagt
//
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.
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