Sec61β-Flag-APEX2-SOPP3 plasmid

PVT99101

Sec61β-Flag-APEX2-SOPP3 plasmid Information

Plasmid name: Sec61β-Flag-APEX2-SOPP3
Alias: (Plasmid #229072)
Gene lenght: 1422bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CMV IE94
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: Sec61β-F：GCTCGTTTAGGGAACCGCCATTC
Reverse primer:
Copy number: High
Induction:

Sec61β-Flag-APEX2-SOPP3 plasmid Description

Sec61β-Flag-APEX2-SOPP3 is a plasmid suitable for protein expression in mammalian cells.

Sec61β-Flag-APEX2-SOPP3 plasmid Sequence

1 CAATTCAATA TGGCGGACTT GGCACCATGC CAATTCAATA TGGCGGACCT GGCACTGTGC 61 CAACTGGGGA GGGGTCTACT TGGCACGGTG CCAAGTTTGA GGAGGGGTCT TGGCCCTGTG 121 CCAAGTCCGC CATATTGAAT TGGCATGGTG CCAATAATGG CGGCCATATT GGCTATATGC 181 CAGGATCAAT ATATAGGCAA TATCCAATAT GGCCCTATGC CAATATGGCT ATTGGCCAGG 241 TTCAATACTA TGTATTGGCC CTATGCCATA TAGTATTCCA TATATGGGTT TTCCTATTGA 301 CGTAGATAGC CCCTCCCAAT GGGCGGTCCC ATATACCATA TATGGGGCTT CCTAATACCG 361 CCCATAGCCA CTCCCCCATT GACGTCAATG GTCTCTATAT ATGGTCTTTC CTATTGACGT 421 CATATGGGCG GTCCTATTGA CGTATATGGC GCCTCCCCCA TTGACGTCAA TTACGGTAAA 481 TGGCCCGCCT GGCTCAATGC CCATTGACGT CAATAGGACC ACCCACCATT GACGTCAATG 541 GGATGGCTCA TTGCCCATTC ATATCCGTTC TCACGCCCCC TATTGACGTC AATGACGGTA 601 AATGGCCCAC TTGGCAGTAC ATCAATATCT ATTAATAGTA ACTTGGCAAG TACATTACTA 661 TTGGAAGTAC GCCAGGGTAC ATTGGCAGTA CTCCCATTGA CGTCAATGGC GGTAAATGGC 721 CCGCGATGGC TGCCAAGTAC ATCCCCATTG ACGTCAATGG GGAGGGGCAA TGACGCAAAT 781 GGGCGTTCCA TTGACGTAAA TGGGCGGTAG GCGTGCCTAA TGGGAGGTCT ATATAAGCAA 841 TGCTCGTTTA GGGAACCGCC ATTCTGCCTG GGGACGTCGG AGCAAGCTTG ATTTAGGTGA 901 CACTATAGAA TACAAGCTAC TTGTTCTTTT TGCAGGATCC ATGCCTGGAC CCGCAGCTAG 961 TGCAACAAAT GTTGGTGCCT CCAGCCGTTC CCCCAGTAAG ACGGTGGCTC CCCGCACTGC 1021 TGGTACCTCA GCCAGACAAA GGAAAGCCAC AAGCAGCAGT GCACGCAGCG GAGGCAGATC 1081 CACAGCTTCT GCAGGCACAG GAGGAATGTG GCGCTTTTAC ACTGAAGATT CACCAGGGCT 1141 TAAAGTTGGC CCAGTTCCAG TTTTGGTGAT GAGTCTGCTG TTTATCGCAT CTGTCTTCAT 1201 GCTGCACATC TGGGGAAAGT ACACCCGCTC CGCTAGCGAC TACAAGGATG ACGATGACAA 1261 AGAATTCGGG AAATCATACC CAACAGTGTC CGCAGACTAC CAGGATGCCG TGGAGAAAGC 1321 CAAGAAGAAG CTGAGAGGGT TTATCGCAGA AAAGAGGTGC GCACCTCTGA TGCTGAGACT 1381 GGCTTTCCAC AGCGCAGGCA CCTTTGACAA GGGAACCAAA ACAGGCGGAC CCTTTGGAAC 1441 AATCAAGCAC CCTGCTGAAC TGGCACATAG TGCCAACAAT GGGCTGGACA TCGCCGTGCG 1501 GCTGCTGGAA CCTCTGAAAG CAGAGTTCCC AATTCTGTCC TACGCCGATT TTTATCAGCT 1561 GGCAGGAGTG GTCGCTGTGG AGGTCACTGG GGGCCCCAAG GTGCCTTTCC ACCCAGGACG 1621 GGAGGACAAA CCAGAACCAC CTCCAGAGGG GCGCCTGCCA GATCCGACAA AGGGCTCCGA 1681 CCATCTGCGA GATGTGTTTG GGAAAGCTAT GGGCCTGACT GACCAGGATA TCGTCGCACT 1741 GTCTGGAGGG CACACCATTG GCGCCGCTCA TAAGGAAAGG TCAGGCTTCG AGGGACCCTG 1801 GACAAGCAAC CCCCTGATTT TCGACAATTC TTACTTTACT GAACTGCTGA GTGGAGAGAA 1861 GGAAGGGCTG CTGCAGCTGC CCAGCGACAA AGCCCTGCTG TCCGATCCCG TGTTCAGACC 1921 TCTGGTCGAT AAGTATGCAG CCGACGAGGA TGCTTTTTTC GCAGATTACG CAGAAGCACA 1981 TCAGAAGCTG TCAGAACTGG GATTTGCCGA CGCCACCGGT GGCGGTGGCG GATCGACTAG 2041 TATGGAGAAA AGTTTCGTGA TAACTGATCC ACGGCTGCCA GACAATCCCA TCATCTTCGC 2101 ATCCGATGGC TTCCTGGAGC TGACCGAGTA TTCCAGAGAG GAGATCCTGG GCCGCAATGG 2161 CCGCTTTCTG CAGGGACCAG AGACAGACCA GGCCACAGTG CAGAAGATTC GCGATGCCAT 2221 TAGAGATCAG CGCGAGATTA CCGTGCAGCT GATAAACTAC ACAAAAAGCG GGAAGAAATT 2281 CTTGAACCTC CTGAACCTCC AGCCCATTAG GGACCAGAAG GGTGAGCTCC AGGCTTTCAT 2341 CGGAGTGGTG CTGGATGGAT AGCTCGAGCC TCTAGAACTA TAGTGAGTCG TATTACGTAG 2401 ATCCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA 2461 AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT 2521 GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT CAGGGGGAGG 2581 TGTGGGAGGT TTTTTAATTC GCGGCCGCGG CGCCAATGCA TTGGGCCCGG TACCCAGCTT 2641 TTGTTCCCTT TAGTGAGGGT TAATTGCGCG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC 2701 TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGGA GCATAAAGTG 2761 TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC 2821 CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG 2881 GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC 2941 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 3001 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 3061 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 3121 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 3181 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 3241 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 3301 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 3361 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 3421 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 3481 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 3541 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 3601 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 3661 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 3721 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 3781 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC 3841 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC 3901 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC 3961 TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC 4021 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC 4081 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT 4141 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC 4201 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA 4261 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT 4321 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG 4381 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC 4441 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA 4501 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT 4561 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT 4621 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG 4681 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA 4741 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT 4801 AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTAAA TTGTAAGCGT TAATATTTTG 4861 TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT TTAACCAATA GGCCGAAATC 4921 GGCAAAATCC CTTATAAATC AAAAGAATAG ACCGAGATAG GGTTGAGTGT TGTTCCAGTT 4981 TGGAACAAGA GTCCACTATT AAAGAACGTG GACTCCAACG TCAAAGGGCG AAAAACCGTC 5041 TATCAGGGCG ATGGCCCACT ACGTGAACCA TCACCCTAAT CAAGTTTTTT GGGGTCGAGG 5101 TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC TTGACGGGGA 5161 AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG 5221 CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC CCGCCGCGCT TAATGCGCCG 5281 CTACAGGGCG CGTCCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG 5341 CGGGCCTCTT CGCTATTACG CCAGTCGACC GCCAATTCAA TATGGCGTAT ATGGACTCAT 5401 GCCAATTCAA TATGGTGGAT CTGGACCTGT GCCAATTCAA TATGGCGTAT ATGGACTCGT 5461 GCCAATTCAA TATGGTGGAT CTGGACCCCA GC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???