Anti-15 Lipoxygenase 2 antibody

Product Name Anti-15 Lipoxygenase 2 antibody

Catalog No PAab00005

Packing 100ug

Form liquid

Alternative Name ALOX15B antibody,Arachidonate 15-lipoxygenase B antibody,15-LOX-B antibody,15-LOX-2 antibody,Arachidonate 15-lipoxygenase type II antibody,Linoleate 13-lipoxygenase 15-Lob

Purification Immunogen affinity purified

Purity 95% as determined by SDS-PAGE

Host Rabbit

Isotype IgG

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

In humans, there are 6 functional lipoxygenases genes. Two of them encode for arachidonic acid 12-lipoxygenating (ALOX12, ALOX12B) and two for 15-lipoxygenating enzymes (ALOX15, ALOX15B). ALOX15B has been described as the most abundant 15-lipoxygenating enzyme species in human carotid atherosclerotic lesions and to be associated with cerebrovascular symptoms. (PMID:24373925). ALOX15B has 4?isoforms?produced by?alternative splicing with the MW of 76, 67, 69 and 73 kDa. 13073-1-AP can also recognize the mouse Alox8 protein.

Immunogen arachidonate 15-lipoxygenase, type B

Specificity Human,Mouse ,Rat

Tested Application ELISA,WB,IHC,IF

Recommended dilution WB : 1:200-1:2000;IHC : 1:20-1:200

IHC use

Immunohistochemistry of paraffin-embedded human prostate cancer usingALOX15B antibody at dilution of 1:100

WB use

MCF7 cells were subjected to SDS PAGE followed by western blot with PAab00005(ALOX15B antibody) at dilution of 1:600

Protein Information

Gene ID247

Uniprot ID O15296

Calculated MW 76kDa

location