Anti-androgen receptor antibody

Product Name Anti-androgen receptor antibody

Catalog No PAab00388

Packing 100ug

Form liquid

Alternative Name AIS antibody, androgen receptor antibody, AR antibody, DHTR antibody, Dihydrotestosterone receptor antibody, HUMARA antibody, HYSP1 antibody, KD antibody, NR3C4 antibody, SBMA antibody, SMAX1 antibody, TFM

Purification Immunogen affinity purified

Purity 95% as determined by SDS-PAGE

Host Rabbit

Isotype IgG

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

Androgen receptor (AR) also konwn as Dihydrotestosterone receptor (DHTR), Nuclear receptor subfamily 3 group C member 4 (NR3C4).It is one of steriod hormoen receptors, which are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.Defects in AR are the cause of androgen insensitivity syndrome (AIS).Affected males have female external genitalia, female breast development, blind vagina, absent uterus and female adnexa, and abdominal or inguinal testes, despite a normal 46,XY karyotype.Defects in AR are the cause of spinal and bulbar muscular atrophy X-linked type 1 (SMAX1). In SMAX1 patients the number of Gln ranges from 38 to 62. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.Defects in AR may play a role in metastatic prostate cancer. The mutated receptor stimulates prostate growth and metastases development despite of androgen ablation. This treatment can reduce primary and metastatic lesions probably by inducing apoptosis of tumor cells when they express the wild-type receptor.Defects in AR are the cause of androgen insensitivity syndrome partial (PAIS). PAIS is characterized by hypospadias, hypogonadism, gynecomastia, genital ambiguity, normal XY karyotype, and a pedigree pattern consistent with X-linked recessive inheritance. Some patients present azoospermia or severe oligospermia without other clinical manifestations.This antibody is a rabbit polyclonal antibody. It can specifically recognize the 110kd AR protein.

Immunogen androgen receptor

Specificity Human,Mouse ,Rat

Tested Application ELISA,WB,IHC

Recommended dilution WB :1:500-1:5000;IHC:1:20-1:200

IHC use

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using AR Antibody at dilution of 1:200. heat mediated antigen retrieved with Tris-EDTA bufferpH9).

WB use

PC-3 cells were subjected to SDS PAGE followed by western blot with PAab00388(AR antibody) at dilution of 1:1000

Protein Information

Gene ID367

Uniprot ID P10275

Calculated MW 99kDa,observed 71kDa