Anti-ATP5J antibody

Product Name Anti-ATP5J antibody

Catalog No PAab00711

Packing 100ug

Form liquid

Alternative Name ATP5A antibody, ATPM

Purification Immunogen affinity purified

Purity 95% as determined by SDS-PAGE

Host Rabbit

Isotype IgG

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes.

Immunogen ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6

Specificity Human, Mouse ,Rat

Tested Application ELISA,WB,IHC

Recommended dilution

IHC use

Immunohistochemistry of paraffin-embedded human osteosarcoma usingATP5J antibody at dilution of 1:100

WB use

human brain tissue were subjected to SDS PAGE followed by western blot with PAab00711(ATP5J antibody) at dilution of 1:800

Protein Information

Gene ID522

Uniprot ID P18859

Calculated MW

location