Anti-BAAT antibody

Product Name Anti-BAAT antibody

Catalog No PAab00780

Packing 100ug

Form liquid

Alternative Name

Purification Immunogen affinity purified

Purity 95% as determined by SDS-PAGE

Host Rabbit

Isotype IgG

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

Involved in bile acid metabolism. In liver hepatocytes catalyzes the second step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi. The major components of bile are cholic acid and chenodeoxycholic acid. In a first step the bile acids are converted to an acyl-CoA thioester, either in peroxisomes (primary bile acids deriving from the cholesterol pathway), or cytoplasmic at the endoplasmic reticulum (secondary bile acids). May catalyze the conjugation of primary or secondary bile acids, or both. The conjugation increases the detergent properties of bile acids in the intestine, which facilitates lipid and fat-soluble vitamin absorption. In turn, bile acids are deconjugated by bacteria in the intestine and are recycled back to the liver for reconjugation (secondary bile acids). May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids. In vitro, catalyzes the hydrolysis of long- and very long-chain saturated acyl-CoAs to the free fatty acid and coenzyme A (CoASH), and conjugates glycine to these acyl-CoAs.

Immunogen bile acid Coenzyme A: amino acid N-acyltransferase (glycine N-choloyltransferase)

Specificity Human

Tested Application ELISA,WB,IF,IHC

Recommended dilution

IHC use

Immunohistochemistry of paraffin-embedded human hepatocirrhosis tissue slide using BAAT Antibody at dilution of 1:100

WB use

human liver tissue were subjected to SDS PAGE followed by western blot with PAab00780(BAAT antibody) at dilution of 1:500

Protein Information

Gene ID570

Uniprot ID Q14032

Calculated MW

location