Anti-CALCOCO2 antibody

Product Name Anti-CALCOCO2 antibody

Catalog No PAab01196

Packing 100ug

Form liquid

Alternative Name NDP52

Purification Immunogen affinity purified

Purity 95% as determined by SDS-PAGE

Host Rabbit

Isotype IgG

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

Xenophagy-specific receptor required for autophagy- mediated intracellular bacteria degradation. Acts as an effector protein of galectin-sensed membrane damage that restricts the proliferation of infecting pathogens such as Salmonella typhimurium upon entry into the cytosol by targeting galectin-8- associated bacteria for autophagy (PubMed:22246324). Initially orchestrates bacteria targeting to autophagosomes and subsequently ensures pathogen degradation by regulating pathogen-containing autophagosome maturation (PubMed:23022382, PubMed:25771791). Bacteria targeting to autophagosomes relies on its interaction with MAP1LC3A, MAP1LC3B and/or GABARAPL2, whereas regulation of pathogen-containing autophagosome maturation requires the interaction with MAP3LC3C (PubMed:23022382, PubMed:25771791). May play a role in ruffle formation and actin cytoskeleton organization and seems to negatively regulate constitutive secretion (PubMed:17635994).

Immunogen calcium binding and coiled-coil domain 2

Specificity Human, Mouse ,Rat

Tested Application ELISA,WB,IHC,IP

Recommended dilution  WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB IHC : 1:20-1:200 IF : 1:20-1:200

IHC use

Immunohistochemistry of paraffin-embedded human ovary tumor usingCALCOCO2 antibody at dilution of 1:100

WB use

HeLa cells were subjected to SDS PAGE followed by western blot with PAab01196(CALCOCO2 antibody) at dilution of 1:1000

Protein Information

Gene ID10241

Uniprot ID Q13137

Calculated MW  52 kDa

location  Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane