Anti-GNAI3 antibody

Product Name Anti-GNAI3 antibody

Catalog No PAab03533

Packing 100ug

Form liquid

Alternative Name

Purification Immunogen affinity purified

Purity 95% as determined by SDS-PAGE

Host Rabbit

Isotype IgG

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:8774883, PubMed:18434541, PubMed:19478087). Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (PubMed:19478087). Stimulates the activity of receptor-regulated K(+) channels (PubMed:2535845). The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division (PubMed:17635935).

Immunogen guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3

Specificity Human,Mouse ,Rat

Tested Application ELISA,WB,IHC,IF

Recommended dilution

IHC use

Immunohistochemistry of paraffin-embedded human kidney usingGNAI3 antibody at dilution of 1:50

WB use

mouse kidney tissue were subjected to SDS PAGE followed by western blot with PAab03533(GNAI3 antibody) at dilution of 1:500

Protein Information

Gene ID2773

Uniprot ID P08754

Calculated MW

location