Anti-NPC1 antibody
Product Name Anti-NPC1 antibody
Catalog No PAab05812
Packing 100ug
Form liquid
Alternative Name
Purification Immunogen affinity purified
Purity 95% as determined by SDS-PAGE
Host Rabbit
Isotype IgG
Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)
Background/ FUNCTION
Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes/ lysosomes to the ER and plasma membrane by an unknown mechanism. Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals. (Microbial infection) Acts as an endosomal entry receptor for ebolavirus.
Immunogen Niemann-Pick disease, type C1
Specificity Human
Tested Application ELISA,WB,IHC
Recommended dilution
IHC use
Immunohistochemistry of paraffin-embedded human brain tissue slide using NPC1 Antibody at dilution of 1:50
WB use
HEK-293 cells were subjected to SDS PAGE followed by western blot with PAab05812(NPC1 antibody) at dilution of 1:1000
Protein Information
Gene ID4864
Uniprot ID O15118
Calculated MW
location