Anti-NPC1 antibody

Product Name Anti-NPC1 antibody

Catalog No PAab05812

Packing 100ug

Form liquid

Alternative Name

Purification Immunogen affinity purified

Purity 95% as determined by SDS-PAGE

Host Rabbit

Isotype IgG

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes/ lysosomes to the ER and plasma membrane by an unknown mechanism. Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals. (Microbial infection) Acts as an endosomal entry receptor for ebolavirus.

Immunogen Niemann-Pick disease, type C1

Specificity Human

Tested Application ELISA,WB,IHC

Recommended dilution

IHC use

Immunohistochemistry of paraffin-embedded human brain tissue slide using NPC1 Antibody at dilution of 1:50

WB use

HEK-293 cells were subjected to SDS PAGE followed by western blot with PAab05812(NPC1 antibody) at dilution of 1:1000

Protein Information

Gene ID4864

Uniprot ID O15118

Calculated MW

location